Physiological quality and gene expression during the development of habanero pepper (Capsicum chinense Jacquin) seeds

Pinho

et al. 2019

JEAI

Currently, the X-ray image analysis of seeds has proved effective for the evaluation of the physical and physiological quality of the seeds, besides being a non-destructive method. The objective of this work was to evaluate the internal free area of jiló seeds (Solanum gilo) through the analysis of radiographic images and to relate the results with the germination of seeds in different stages of maturation. The seeds were harvested at 35, 40, 45 days after anthesis and 45 days after anthesis with 7 days at rest (45 DAAr). Seeds with free internal area ≤ 10% produced normal seedlings whereas seeds with an average free area above 10% produced abnormal or non-germinated seedlings. Seeds of fruits harvested at 45 DAA and seven days at rest showed a smaller internal free area and a formation of 100% of normal seedlings. The analysis of the radiographic images of jiló seeds allows the measurement of the free internal areas, as well as the determination of the relation between these and the germination. Alves et al.; JEAI, 35(6): 1-10, 2019; Article no.JEAI.48682 2 Original Research Article

Section: Germination Testsupporting

confidence: 85%

Image Analysis in the Evaluation of the Physical and Physiological Quality of Jiló (Solanum gilo) Seeds during Development

Pinho

et al. 2019

JEAI

“…Similar results as for the gradual increase in the percentages of germination, emergence and germination speed in the different stages of maturation were found for the green pepper [22], Jiló [27], cucumber (Cucumis sativus L.) [20], green pepper; pumpkin [28], pepper (Capsicum baccatum L.) [23] [24], (Capsicum chinense JACQUIM) [29], Capsicum baccatum var. pendulum [30].…”

Section: Emergence and Emergence Speed Indexsupporting

confidence: 74%

“…Results were found by Santos et al [29], where the highest expression of the enzyme isocitrate lyase was found in habanero pepper seeds during the later stages of development.…”

Section: Isocitrate Lyasementioning

confidence: 73%

Physiological and Biochemical Characterization of Jiló Seeds (Solanum gilo) in Different Harvest Times

Alves¹,

Pinho²,

Santos³

et al. 2017

AJPS

The evaluation of seed quality during the development process is important for the definition of the moment of harvest. In this context, the objective in this study was to evaluate the quality of Jiló seeds during development through physiological, biochemical and electrophoretic tests of enzymes. Six genetic materials, JIL white, JIL 001, JIL 005, JIL 006, H1 and H2 were used. Fruits were harvested at 35, 40, 45 days after anthesis (DAA). A total of 50% of all fruits harvested at 45 DAA remained at rest for seven days which were labeled as 45 DAAr. The seeds at each maturation stage were extracted from the fruits and evaluated by water content, weight of one thousand seeds, electric conductivity, germination, germination speed index, seedling emergence, and seedling emergence rate index. The chemical composition of the seeds was also evaluated, as well as the expression of the enzymes esterase, isocitrate lyase, malate dehydrogenase, alcohol dehydrogenase, superoxide dismutase, catalase, peroxidase and endo-β-mannanase. The highest values of germination and vigor were found in seeds harvested at 45 DAA and 45 DAAr, mainly for hybrids H1 and H2. The highest values of lipids and proteins and lower carbohydrates were found in the most immature seeds of Jiló. The enzymes analyzed showed higher expression in the initial stages of development, however reduced with the advancement of the maturation stage. Endo-β-mannanase expression increased with seed development. Jiló seeds harvested at 45 DAAr presented the highest of germination and vigor values. This "being" considered as the ideal harvest point.

“…Vegetable oils are the foremost among all oil products exhibiting speci c bene cial and functional properties towards human [8]. In oil crops, accumulating research reported that the phytohormones exhibit a signi cant effect on seed germination, seedling growth, and yield [9][10][11], thus the quanti cation of the phytohormones is critical for the underlying regulation mechanism. However, the analysis of phytohormones in oilseeds matrices is yet a challenging issue, because of the inherent complexity of the matrix.…”

Section: Introductionmentioning

confidence: 99%

Magnetic Solid-Phase Extraction and in Situ Derivatization for Determining Phytohormones and in Oilseeds by Ultra-Performance Liquid Chromatography-tandem Mass Spectrometry

Luo

Wang

et al. 2020

Preprint

Background: Phytohormones are a group of naturally occurring signaling molecules which influence physiological processes of oil crops. Simultaneous determination of multiple phytohormones in oilseeds is still a challenge due to their trace concentrations, species diversity, and lipid interference. Therefore, a simple and selective method for the simultaneous determination of multiple phytohormones in oilseeds is urgently needed.Results: In this study, the Fe3O4@Ti3C2@β-CD nanoparticles were successfully synthesized and used for the first time as an adsorbent for the magnetic dispersive solid-phase extraction of phytohormones from oilseeds. The magnetic dispersive solid-phase extraction and in situ derivation by the addition of N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide (EDC) were ingeniously combined. This efficient pre-treatment method integrated the extraction, purification, and derivatization processes into one single step. Several parameters affecting the efficiency of extraction and derivatization were evaluated. Under the optimized analysis conditions, satisfactory methodological performance was achieved. High linearities (R2 > 0.9928) at three spiked levels, as well as the low matrix effect (ranged from 16.63 % to 17.06 %) and limits of detection (0.89-13.62 pg/mL) were also obtained. The intra and inter-day relative standard deviations (RSDs) were less than 13.7 % and 11.6 %, respectively. The recoveries were ranged from 80.4 % to 115.1 %. This method was successfully employed for analyzing 12 phytohormones in different oilseeds samples.Conclusion: A simple and sensitive method based on the magnetic solid phase extraction integrated with in situ derivations for the profiling of 12 phytohormones, including 9 gibberellins (GAs), indole-3-acetic acid (IAA), abscisic acid (ABA), and jasmonic acid (JA) in a single rapeseed seed was developed by using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS).