Physiological response of the moderately halophilic psychrotolerant strain Chromohalobacter sp. N1 to salinity change and low temperature

Abstract

The available information on de novo synthesized compatible solutes in response to high medium salinity by bacteria of the Chromohalobacter genus is limited to studies of the mesophilic moderately halophilic strain Chromohalobacter salexigens DSM 3043T. Therefore, there is a need for studies of representatives of other species of the Chromohalobacter genus of the Halomonadaceae family. A moderately halophilic psychrotolerant bacterium, strain N1, closely related to the species Chromohalobacter japonicus was isolated from the salt crust of a rock salt waste pile in Berezniki, Perm Krai, Russia. An intracellular pool of compatible solutes of strain N1 was investigated by NMR spectroscopy. Cells grown in the presence of 5% NaCl at optimal growth temperature (28 °C) accumulated ectoine, glutamate, N(4)-acetyl-l-2,4-diaminobutyrate (NADA), alanine, trehalose, hydroxyectoine, and valine. Such a combination of compatible solutes is unique and distinguishes the strain from C. salexigens DSM 3043T. Hyperosmotic stress induced by 15% NaCl caused the accumulation of ectoine, NADA, and hydroxyectoine but led to a decrease in the amount of alanine, valine, and trehalose. The intracellular pool of glutamate was not significantly changed. A reduction of the growth temperature from 28 to 5 °C led to an increase in the amount of ectoine, NADA, trehalose, and hydroxyectoine. Ectoine was the major compatible solute.

Impact of key parameters involved with plant-microbe interaction in context to global climate change

“…A 6% NaCl content triggered EPS production in Tetragenococcus halophilus isolated from soya sauce moromi with 52.7% recovery (both fractions) consisting of glucose, galactose, mannose, and glucuronic acid (Zhang et al, 2022). Similarly, Chromohalobacter japonicus isolated from a rock salt waste accumulated ectoine, trehalose, N(4)-acetyl-l-2,4diaminobutyrate, alanine, valine hydroxyectoine, and glutamate (compatible solutes) in the presence of 5% NaCl (Ananina et al, 2021). However, high salinity of 25% NaCl at pH 10.07 induced carotenoid pigmentation (0.98 g/l) in Natrialba sp.…”

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“…grows optimally at 110 °C at 3 M NaCl. Psychrotolerant halophile, Chromohalobacter salexigens grows 4 M NaCl at 0 °–35 °C and produce 5-hydroxyectoine to avoid desiccation [ 4 ]…”