Physiological response to heat stress of tomato ‘Micro-Tom’ plants expressing high and low levels of mitochondrial sHSP23.6 protein

Hüther, Cristina Moll; Ramm, Aline; Rombaldi, César Valmor; Bacarin, Marcos Antônio

doi:10.1007/s10725-013-9790-y

Cited by 20 publications

(12 citation statements)

References 51 publications

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“…The low transcription and protein amount required may be due to a low number of processes they are involved. Thus, although this protein is located in the mitochondria, it is believed to act within the organelle it self to promote homeostasis of the plastids, as reported by our group in previous studies with MT-sHSP23.6 sense tomato Micro-Tom genotype, lower photosynthetic damage under high temperature in comparison to antisense genotype, suggest in a high tolerance degree to heat due to these protein function (Huther et al, 2013). It is known that these two organelles interact during redox depletion, the interchange of metabolites, energy and sub-cellular protection (Waters, 2013).…”

Section: Introductionsupporting

confidence: 62%

“…Micro-Tom) were used in the experiment. The seeds were either untransformed or had been transformed to various expression levels of MT-sHSP23.6 mitochondrial sense and antisense, as described by Huther et al (2013).…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

“…Prior studies (Rampino et al, 2009;Huther et al, 2013) have found that the HSP23.6 gene is typically expressed in response to various pressures, and its expression levels are strongly up-regulated during some developmental stages (Sarkar et al, 2009). Slight increase in HSP23.6 gene transcription was reported in tomato fruits under hypoxia (Pegoraro et al, 2012).…”

Section: Introductionmentioning

confidence: 97%

“…One type of HSP, small heat shock proteins (sHSP), are produced in response to a wide variety of environmental stressors and act with other HSP to control stress prevention and recovery (Waters, 2013;Huther et al, 2013). HSP act on protein synthesis and repair and protect the endomembrane system, helping to restore cellular homeostasis (Pegoraro et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Effects of flooding stress in ‘Micro-Tom’ tomato plants transformed with different levels of mitochondrial sHSP23.6

et al. 2017

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Soil flooding is an environmental stressor for crops that can affect physiological performance and reduce crop yields. Abiotic stressors cause changes in protein synthesis, modifying the levels of a series of proteins, especially the heat shock proteins (HSP), and these proteins can help protect the plants against abiotic stress. The objective of this study was to verify if tomato plants cv. Micro-Tom from different genotypes with varying expression levels of MT-sHSP23.6 (mitochondrial small heat shock proteins) have different responses physiological to flooding. Plants from three genotypes (untransformed, MT-sHSP23.6 sense expression levels and MT-sHSP23.6 antisense expression levels) were cultivated under controlled conditions. After 50 days, the plants were flooded for 14 days. After this period half of the plants from each genotype were allowed to recover. Chlorophyll fluorescence, gas exchange, chlorophyll index, leaf area and dry matter were evaluated. Flood stress affected the photosynthetic electron transport chain, which is related to inactivation of the oxygen-evolving complex, loss of connectivity among units in photosystem II, oxidation-reduction of the plastoquinone pool and activity of photosystem I. The genotype with MT-sHSP23.6 sense expression levels was less sensitive to stress from flooding.Keywords: Solanum lycopersicum Mill, photosynthesis, chlorophyll fluorescence, stress.Efeitos do estresse por alagamento em plantas de tomateiro 'Micro-Tom' transformada com diferentes níveis de sHSP23.6 mitocondrial ResumoO alagamento do solo é um estressor ambiental para as culturas e pode afetar o desempenho fisiológico e reduzir a produtividade das culturas. Estresses abióticos causam mudanças na síntese de proteínas, modificando os níveis de uma série de proteínas, em especial as proteínas de choque térmico (HSP) e essas proteínas são conhecidas por proteger as plantas contra estresses abióticos. O objetivo deste estudo foi verificar se as plantas do tomateiro cv. Micro-Tom de distintos genótipos com diferentes níveis de expressão da MT-sHSP23.6 (proteínas mitocondriais de choque térmico com pequena massa molecular), têm diferentes respostas fisiológicas ao alagamento. As plantas de três genótipos (não-transformado, transformado com orientação antisense e transformado com orientação sense para MT-sHSP23.6) foram cultivadas sob condições controladas. Após 50 dias as plantas foram alagadas durante 14 dias. Após esse período as plantas de cada genótipo foram recuperadas. Foram avaliados fluorescência da clorofila, trocas gasosas, índice de clorofila, área foliar e massa seca. O estresse por alagamento afetou a cadeia de transporte de elétrons da fotossíntese, que está relacionado à inativação do complexo de evolução do oxigênio, perda da conectividade entre as unidades do fotossistema II, de oxidação e redução do pool de plastoquinona e atividade do fotossistema I. O genótipo com orientação sense MT-sHSP23.6 foi menos sensível ao estresse por alagamento.Palavras-chave: Solanum lycopersicum Mill, f...

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Section: Introductionsupporting

confidence: 62%

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Effects of flooding stress in ‘Micro-Tom’ tomato plants transformed with different levels of mitochondrial sHSP23.6

et al. 2017

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“…A heat-inducible ER-sHSP has been demonstrated to perform a molecular chaperone function in vitro (Mamedov and Shono, 2008). Finally, Huther et al (2013) have verified that MT-sHSP23.6 is involved in heat tolerance. In their study, transgenic plants suffered greater physiological damage during heat stress as a consequence of MT-sHSP23.6 suppression.…”

Section: Discussionmentioning

confidence: 78%

Genome-wide identification, classification, and expression analysis of sHSP genes in Chinese cabbage (Brassica rapa ssp pekinensis)

Peng

Guo²,

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Small heat shock proteins (sHSPs) are essential for the plant's normal development and stress responses, especially the heat stress response. The information regarding sHSP genes in Chinese cabbage (Brassica rapa ssp pekinensis) is sparse, hence we performed a genome-wide analysis to identify sHSP genes in this species. We identified 26 non-redundant sHSP genes distributed on all chromosomes, except chromosome A7, with one additional sHSP gene identified from an expressed sequence tag library. Chinese cabbage was found to contain more sHSP genes than Arabidopsis. The 27 sHSP genes were classified into 11 subfamilies. We identified 22 groups of sHSP syntenic orthologous genes between Chinese cabbage and Arabidopsis. In addition, eight groups of paralogous genes were uncovered in Chinese cabbage. Protein structures of the 27 Chinese cabbage sHSPs were modeled using Phyre2, which revealed that all of them contain several conserved β strands across different subfamilies. In general, gene structure was conserved within each subfamily between P. Tao et al.

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Quantitative trait locus (QTL) mapping and transcriptome profiling identify QTLs and candidate genes associated with heat stress response during reproductive development in Camelina sativa

Smith,

Kemmer,

Decker

et al. 2024

Food and Energy Security

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Camelina sativa (L.) Crantz is a low‐input oilseed crop that has great potential in providing sustainable feedstock for biofuels and bioproducts. Climate change is threatening production of camelina with rising global temperatures. Elucidating the genetic response to high temperatures is essential for successful breeding of heat‐tolerant camelina varieties. Here, we report a combinatorial approach to identifying candidate genes associated with heat stress by quantitative trait locus (QTL) mapping and comparative transcriptome profiling. A population of recombinant inbred lines (RILs) was grown in a controlled growth chamber under the high‐temperature regimes for 14 days beginning at the onset of the reproductive stage. Several traits related to seed production were evaluated at maturity. The QTL analysis identified several regions with co‐located traits on chromosomes 8, 10, and 12. Two RILs with contrasting phenotypic responses to heat stress were chosen for gene expression profiling via RNA sequencing. Multiple pathways and genes were found to be strongly affected by heat stress, and many genes expressed differently between the two RILs. Several genes identified within the QTL regions were considered strong candidates that may control heat tolerance during reproduction in camelina. These studies provide resources for future studies that may assist in improving the heat tolerance of camelina.

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Physiological response to heat stress of tomato ‘Micro-Tom’ plants expressing high and low levels of mitochondrial sHSP23.6 protein

Cited by 20 publications

References 51 publications

Effects of flooding stress in ‘Micro-Tom’ tomato plants transformed with different levels of mitochondrial sHSP23.6

Effects of flooding stress in ‘Micro-Tom’ tomato plants transformed with different levels of mitochondrial sHSP23.6

Genome-wide identification, classification, and expression analysis of sHSP genes in Chinese cabbage (Brassica rapa ssp pekinensis)

Quantitative trait locus (QTL) mapping and transcriptome profiling identify QTLs and candidate genes associated with heat stress response during reproductive development in Camelina sativa

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