Physiological role of extracellular ribonucleases of higher plants

Sangaev, S. S.; Кочетов, А. В.; Ibragimova, S. S.; Levenko, B. A.; Shumny, V. K.

doi:10.1134/s2079059711010060

Cited by 14 publications

(12 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While the dsRNA that was formulated with the carbon dots, was still intact after a 60-minute incubation with RNase III. The enhanced resistance to nucleases could significantly enhance efficacy in plants, which contain high levels of RNase activity in the extracellular apoplast (Sangaev et al, 2011).…”

Section: Carbon Dots Provide Protection Form Nucleasesmentioning

confidence: 99%

Carbon dots for efficient siRNA delivery and gene silencing in plants

Schwartz

Hendrix

Hoffer

et al. 2019

Preprint

View full text Add to dashboard Cite

The Initiation of RNA interference (RNAi) by topically applied double stranded RNA (dsRNA) has potential applications for plant functional genomics, crop improvement and crop protection. The primary obstacle for the development of this technology is efficient delivery of RNAi effectors. The plant cell wall is a particularly challenging barrier to the delivery of macromolecules. Many of the transfection agents that are commonly used with animal cells produce nanocomplexes that are significantly larger than the size exclusion limit of the plant cell wall. Utilizing a class of very small nanoparticles called carbon dots, a method of delivering siRNA into the model plant Nicotiana benthamiana and tomato is described. Low-pressure spray application of these formulations with a spreading surfactant resulted in strong silencing of GFP transgenes in both species. The delivery efficacy of carbon dot formulations was also demonstrated by silencing endogenous genes that encode two sub-units of magnesium chelatase, an enzyme necessary for chlorophyll synthesis. The strong visible phenotypes observed with the carbon dot facilitated delivery were validated by measuring significant reductions in the target gene transcript and/or protein levels. Methods for the delivery of RNAi effectors into plants, such as the carbon dot formulations described here, could become valuable tools for gene silencing in plants with practical applications in plant functional genomics and agriculture.

show abstract

Section: Carbon Dots Provide Protection Form Nucleasesmentioning

confidence: 99%

Carbon dots for efficient siRNA delivery and gene silencing in plants

Schwartz

Hendrix

Hoffer

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…The second significant association was found with the SNP S13_23674227, which is located in an extracellular ribonuclease gene (RNase gene). The RNase genes in plants have been studied for years and play an essential role in plant defense [39] and development due to their ability to modify RNA levels and thereby influence protein synthesis [40]. Finally, the SNP S13_25522088 was also significantly associated with RL and LXL, but further studies are necessary to determine its role, if any, in regulating these traits.…”

Section: Association Analysismentioning

confidence: 99%

Genome-wide association study (GWAS) for morphological and yield-related traits in an oil palm hybrid (Elaeis oleifera x Elaeis guineensis) population

Osorio

Garzón

Delgadillo

et al. 2019

Preprint

View full text Add to dashboard Cite

Background The genus Elaeis has two species of economic importance for the oil palm agroindustry: Elaeis oleifera (O), native to the Americas, and Elaeis guineensis (G), native to Africa. This work provides to our knowledge, the first association mapping study in an interspecific OxG oil palm population, which shows tolerance to pests and diseases, high oil quality, and acceptable fruit bunch production. Results Using genotyping-by-sequencing (GBS), we identified a total of 3,776 single nucleotide polymorphisms (SNPs) that were used to perform a genome-wide association analysis (GWAS) in 378 OxG hybrid population for 10 agronomic traits. Twelve genomic regions (SNPs) were located near candidate genes implicated in multiple functional categories, such as tissue growth, cellular trafficking, and physiological processes. Conclusions We provide new insights on genomic regions that mapped on candidate genes involved in plant architecture and yield. These potential candidate genes need to be confirmed for future targeted functional analyses. Associated markers to the traits of interest may be valuable resources for the development of marker-assisted selection in oil palm breeding. Keywords: Association mapping, Elaeis guineensis , Elaeis oleifera , genotyping-by-sequencing, plant architecture, yield.

show abstract

“…RNase genes have been studied for years in plants, playing an important role in plant defense mechanism [44] or plant development due to their ability to modify RNA levels, and thereby influence protein synthesis [45]. The SNP S13_25522088 was also significantly associated with RL and LXL, but further studies are necessary to determine their role, if any, in regulating these traits.…”

Section: Gbsmentioning

confidence: 99%

Genome wide association study (GWAS) for morphological and yield-related traits in oil palm hybrids (Elaeis oleifera x Elaeis guineensis)

Osorio

Garzón

Delgadillo

et al. 2019

Preprint

View full text Add to dashboard Cite

Background The genus Elaeis has two species of economic importance for the oil palm agroindustry: Elaeis oleifera (O), native to the Americas, and Elaeis guineensis (G), native to Africa. The work presented herein provides, to our knowledge, the first association mapping study in an interspecific OxG hybrid population of oil palm which presents tolerance to pests and diseases, high oil quality, and acceptable fruit bunch production. Results Using genotyping-by-sequencing (GBS), we identified a total of 3,776 single nucleotide polymorphisms (SNPs) that were used to perform a genome-wide association analysis (GWAS) in 378 OxG hybrids for 10 agronomic traits. Twelve genomic regions were located near candidate genes implicated in multiple functional categories, such as tissue growth, cellular trafficking, and physiological processes. Conclusions We provide new insights on candidate genes that mapped on genomic regions involved in plant architecture and yield; however, these potential candidate genes need to be confirmed for future targeted functional analysis. The associated markers may be valuable resources for the development of marker-assisted selection in oil palm breeding. Keywords: Association mapping, Elaeis guineensis, Elaeis oleifera, genotyping-by-sequencing, plant architecture, yield.

show abstract

Physiological role of extracellular ribonucleases of higher plants

Cited by 14 publications

References 40 publications

Carbon dots for efficient siRNA delivery and gene silencing in plants

Carbon dots for efficient siRNA delivery and gene silencing in plants

Genome-wide association study (GWAS) for morphological and yield-related traits in an oil palm hybrid (Elaeis oleifera x Elaeis guineensis) population

Genome wide association study (GWAS) for morphological and yield-related traits in oil palm hybrids (Elaeis oleifera x Elaeis guineensis)

Contact Info

Product

Resources

About