Physiologically Active Compounds in Four Species of <i>Phellinus</i>

Sułkowska-Ziaja, Katarzyna; Maślanka, Anna; Szewczyk, Agnieszka; Muszyńska, Bożena

doi:10.1177/1934578x1701200313

Cited by 59 publications

(62 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bark extracts were subjected to HPLC-DAD analysis [2,3,53,54] using a Merck-Hitachi liquid chromatograph (LaChrom Elite) containing a diode-array detection (DAD) detector (L-2455). A Purospher RP-18e column was used (5 µm, 250 × 4 mm; Merck, Berlin, Germany).…”

Section: Analyses Of Phenolic Compoundsmentioning

confidence: 99%

See 1 more Smart Citation

Malus baccata var. gracilis and Malus toringoides Bark Polyphenol Studies and Antioxidant, Antimicrobial and Anticancer Activities

et al. 2020

View full text Add to dashboard Cite

Exploring new sources of polyphenols with biological activities that work against human diseases is the target of natural product studies. This study determined the polyphenol composition of the bark of Malus species M. baccata var. gracilis (Rehder) T.C.Ku and M. toringoides (Rehder) Hughes, using high-performance liquid chromatography with a diode-array detector (HPLC-DAD) analysis. The antiproliferative, cytotoxic, antioxidant and antimicrobial applications of these extracts, as well as the identified phenol, were studied. The HPLC-DAD analysis confirmed three polyphenols in the extracts out of the 21 screened compounds: protocatechuic acid, gallic acid, and catechin. The major constituents in M. baccata and M. toringoides were protocatechuic acid, at 3.16 and 7.15 mg 100 g−1 dry weight (DW), respectively, and catechin, at 5.55 and 6.80 mg 100 g−1 DW, respectively. M. baccata and M. toringoides bark extracts showed antioxidant activities using 2,2-diphenyl-1-picrylhydrazyl (DPPH), β-carotene bleaching, and ferric-reducing antioxidant power (FRAP) assays, which were attributed to the dominance of protocatechuic acid. The highest antiproliferative and cytotoxic effects were against Jurkat cells. Against MCF-7 and Hela cells, there was necrotic cell accumulation in the early apoptotic as well as the late apoptotic phase. The bark extracts showed noticeable antibacterial effects against Listeria monocytogenes, Bacillus cereus, and Escherichia coli. Protocatechuic acid showed comparable results to bark extracts. There were antifungal effects against Aspergillus ochraceus, A. niger, and Candida albicans, and the activities were higher than the commercial reagent. M. baccata and M. toringoides could be considered as a new source of phenolic acids, including protocatechuic acid with anticancer, antibacterial antifungal, and antioxidant-promising effects.

show abstract

Section: Analyses Of Phenolic Compoundsmentioning

confidence: 99%

“…A flow rate was set at 1 mL min −1 at 25 • C. The extract volume of 20 µL was used for the injection. The compounds were detected at 254 nm by the validated HPLC method [53,54]. Compound identification was accomplished by comparing UV retention times and spectra of reference substances.…”

Section: Analyses Of Phenolic Compoundsmentioning

confidence: 99%

Malus baccata var. gracilis and Malus toringoides Bark Polyphenol Studies and Antioxidant, Antimicrobial and Anticancer Activities

et al. 2020

View full text Add to dashboard Cite

show abstract

“…The stem extracts were studied by HPLC-DAD method [1,2,45,46] using the Merck-Hitachi liquid chromatograph (LaChrom Elite) with a DAD detector (L-2455) in a ultraviolet (UV)-visible spectra range of 200-400 nm (detection wavelength for all compounds was set at 254 nm). The Purospher RP-18e (250 × 4 mm; 5 µm, Merck) column was used and the temperature was set at 25 • C. The mobile phase consisted of A-methanol, B-methanol: 0.5% acetic acid 1:4 (v/v).…”

Section: Analyses Of Phenolic Compoundsmentioning

confidence: 99%

“…The applied HPLC method was previously validated by our group [45,46]. The tested parameters were the following: accuracy; precision at three levels of standard substance concentrations in solution, 50%, 100%, and 150%; linearity; limit of detection (LOD); and limit of quantification (LOQ) [45,46]. Identification of compounds was performed either by comparison with UV spectra and retention times of reference substances or using co-chromatography.…”

Section: Analyses Of Phenolic Compoundsmentioning

confidence: 99%

See 1 more Smart Citation

Mammillaria Species—Polyphenols Studies and Anti-Cancer, Anti-Oxidant, and Anti-Bacterial Activities

et al. 2019

View full text Add to dashboard Cite

Discovering new natural resources of polyphenols is the aim of many recent studies in the field of natural product research. This study tentatively investigated the polyphenols profile of the stems of seven Mammillaria species (M. rhodantha, M. spinosissima, M. hahniana, M. crucigera, M. candida, M. albilanata, and M. muehlenpfordtii) using high performance liquid chromatography with DAD detector (HPLC-DAD) method. Furthermore, the anti-cancer, anti-oxidant, and anti-bacterial potentials of these extracts as well as major identified phenols were explored. The HPLC-DAD study confirmed the availability of six phenolic acids, including gentisic acid, chlorogenic acid, caffeic acid, protocatechuic acid, sinapic acid, and p-hydroxybenzoic acid. The dominant compounds were: gentisic acid in M. rhodantha and M. spinosissima; chlorogenic acid in M. muehlenpfordtii, M. crucigera, and M. rhodantha; and caffeic acid in M. rhodantha, M. crucigera, and M. spinosissima. Stems of Mammillaria sp. showed antiproliferative effects against HeLa, MCF-7, and Jurkat cells. In HeLa and MCF-7 cells, the best antiproliferative activities were found in the treatments with M. rhodantha, M. spinosissima, and M. muehlenpfordtii. The apoptotic assay of M. rhodantha, M. spinosissima, and M. muehlenpfordtii showed accumulation of necrotic cells in the early and late apoptotic phase. M. rhodantha, M. spinosissima, and M. muehlenpfordtii showed the highest anti-oxidant activities using 2,2-diphenyl-1-picrylhydrazyl (DPPH), β-carotene bleaching, and ferric reducing anti-oxidant power (FRAP) assays. M. rhodantha was the best source of antioxidants. Mammillaria sp. showed moderate anti-bacterial effects against bacteria and the highest effects were found using the extracts of M. rhodantha, M. spinosissima, M. crucigera and M. muehlenpfordtii against most bacteria. The anti-bacterial activities were attributed to other phenolic compounds (e.g., chlorogenic acid) than gentisic acid, which was not active against most bacteria. Mammillaria sp. could be considered to be an important natural source of phenolic acids with anti-cancer, anti-bacterial, and anti-oxidant activities.

show abstract

Selected Species of the Genus Phellinus – Chemical Composition, Biological Activity, and Medicinal Applications

Sułkowska-Ziaja

Balik

Muszyńska

2021

Chemistry & Biodiversity

Self Cite

View full text Add to dashboard Cite

This study presents the current knowledge on chemical composition, biological activity, and possible medicinal applications of Phellinus igniarius, Phellinus pini, Phellinus pomaceus, and Phellinus robustus. These inedible arboreal species are phytopathogens that cause the enzymatic decomposition of wood. These species belong to the medicinal mushrooms and have been known for centuries in the traditional medicine of the Far East. They have been used as an effective remedy for stomach and intestinal ailments, diarrhea, and hemorrhages. Mycochemical studies have proved the presence of polysaccharides, phenolic compounds, and terpenoids. These compounds show biological activities such as anticancer, antioxidant, antiangiogenic, and antiviral. Research studies conducted using modern analytical methods have advanced the knowledge on the potential therapeutic use of compounds isolated not only from the fruiting bodies but also from biomass obtained with in vitro biotechnological methods.

show abstract

Physiologically Active Compounds in Four Species of Phellinus

Cited by 59 publications

References 18 publications

Malus baccata var. gracilis and Malus toringoides Bark Polyphenol Studies and Antioxidant, Antimicrobial and Anticancer Activities

Malus baccata var. gracilis and Malus toringoides Bark Polyphenol Studies and Antioxidant, Antimicrobial and Anticancer Activities

Mammillaria Species—Polyphenols Studies and Anti-Cancer, Anti-Oxidant, and Anti-Bacterial Activities

Selected Species of the Genus Phellinus – Chemical Composition, Biological Activity, and Medicinal Applications

Contact Info

Product

Resources

About