2023
DOI: 10.3390/life13081619
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Physiologically Aggregated LacZ Applied in Trehalose Galactosylation in a Recycled Batch Mode

Martina Belkova,
Tatiana Janegova,
Eva Hrabarova
et al.

Abstract: Galactooligosaccharides obtained via β-galactosidase transgalactosylation have health-promoting properties and are widely recognized as effective prebiotics. Trehalose-based galactooligosaccharides could be introduced into food and pharmaceutical industries similarly to trehalose. In light of this, new technological approaches are needed. Recently, in vivo enzyme immobilizations for recombinant proteins have been introduced, and physiological aggregation into active inclusion bodies (aIBs) has emerged as one s… Show more

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