Physiology of spermatozoa at high dilution rates: the influence of seminal plasma

Maxwell, W.M.C.; Johnson, Lawrence A.

doi:10.1016/s0093-691x(99)00222-8

Cited by 224 publications

(173 citation statements)

References 32 publications

Supporting

Mentioning

153

Contrasting

Unclassified

Order By: Relevance

“…Specific seminal plasma components have been described as decapacitation factors in humans (Zhu et al, 2006), mice (Huang et al, 2007), and other species (as reviewed in Tö pferPetersen et al, 1998) and are assumed to protect sperm from factors in the female tract that could trigger early capacitation, thus reducing the possibility of spermoocyte binding. These suggestions are consistent with the observations that preincubation of sperm from the Sperm-Peak fraction with seminal plasma from the Sperm-Free fraction reduced oocyte penetration rate in vitro (Zhu et al, 2000), whereas inclusion of seminal plasma in sperm samples sorted by flow cytometry increased the percentage of uncapacitated, acrosomeintact sperm and reduced oocyte penetration rate in vitro (Maxwell and Johnson, 1999). In the present experiment, total protein concentration of seminal plasma was negatively correlated with both zona pellucida penetration rate and number of sperm penetrating the zona, suggesting that an increase in total protein content produces a predominantly decapacitation effect.…”

Section: Discussionsupporting

confidence: 90%

Seminal Plasma Proteins as Potential Markers of Relative Fertility in Boars

Novak

Sánchez

Dixon

et al. 2010

Journal of Andrology

View full text Add to dashboard Cite

This study investigated whether specific proteins from distinct seminal plasma fractions of boars could be related to in vivo fertility. Nine boars with acceptable sperm motility and morphology for use in artificial insemination demonstrated major differences in total number born and pregnancy rate when low sperm doses (1.5 billion sperm) were used to breed a minimum of 50 gilts per boar. The 2 lowest-fertility and 2 highest-fertility boars were chosen for evaluation of specific seminal plasma proteins. On 4 occasions, semen was collected and separated into 3 fractions based on sperm concentration (Sperm-Peak, Sperm-Rich, and Sperm-Free), and the fractions were analyzed for total protein concentration and abundance of major seminal plasma glycoprotein (PSP-I), AWN-1, and osteopontin protein using Western blotting techniques. The concentrations of these seminal plasma proteins were lower in the Sperm-Peak fractions compared with the SpermFree fractions (P , .05). Seminal plasma from the pooled SpermRich fraction used for artificial insemination was also subjected to two-dimensional gel electrophoresis to investigate novel protein markers related to in vivo fertility. Total piglets born (r 5 20.76, P 5 .01) and sperm motility at day 7 (r 5 20.74, P 5 .037) were again negatively correlated with a 22-kDa protein identified by mass spectrometry as PSP-I. However, fertility index and farrowing rate tended to be positively correlated (P , .10) with a 25-kDa protein, identified as glutathione peroxidase (GPX5), an antioxidant enzyme that may protect sperm membranes from oxidative damage. These candidate proteins merit further investigation as markers of fertility in boars.

show abstract

Section: Discussionsupporting

confidence: 90%

Seminal Plasma Proteins as Potential Markers of Relative Fertility in Boars

Novak

Sánchez

Dixon

et al. 2010

Journal of Andrology

View full text Add to dashboard Cite

show abstract

“…At high dilution levels, as for bull and ram semen, sperm function declines, a phenomenon called the "dilution effect" [Mann and Lutwak-Mann (1981) in Maxwell and Johnson (1999)], which may be due to reduced contact between sperm and components of the male reproductive tract (Garner et al 2001).…”

Section: The Cryopreservation Processmentioning

confidence: 99%

Semen cryopreservation: Successes and persistent problems in farm species

Bailey

Morrier²,

Cormier³

2003

Can. J. Anim. Sci.

121

View full text Add to dashboard Cite

Bailey, J., Morrier, A. and Cormier. N. 2003. Semen cryopreservation: Successes and persistent problems in farm species. Can. J. Anim. Sci. 83: 393-401. Artificial insemination has arguably been the most important practice contributing to the advancement of animal production. The numerous advantages of artificial insemination are augmented when the semen is cryopreserved and conserved for long periods. Unfortunately, the utility of cryopreserved semen is limited because for most mammals, even cattle, a considerable proportion of sperm loose their fertility during freezing-thawing. In many species, this loss of fertility is substantial, rendering cryopreserved semen impractical for routine use. Our goals are to characterize the nature of the sublethal sperm damage caused by cryopreservation, and to develop a method to improve the functional fertility of thawed semen. This review describes our theory that sperm are prematurely activated by cryopreservation ("cryo-capacitation") and discusses strategies for preventing this damage.Key words: Sperm, artificial insemination, capacitation, fertility Bailey, J., Morrier, A. et Cormier, N. 2003. La cryoconservation de la semence : les succès et les problèmes persistants chez les espèces de la ferme. Can. J. Anim. Sci. 83: 393-401. L'insémination artificielle a grandement contribué à l'avancement des productions animales. Les nombreux avantages qu'offre cette technique sont augmentés lorsque la semence est cryoconservée et entreposée pour de longues périodes. Malheureusement, l'utilisation de la semence cryoconservée est limitée puisque chez la majorité des mammifères, incluant la vache, une large proportion des spermatozoïdes perdra leur motilité pendant le processus de congéla-tion-décongélation. Chez plusieurs espèces, cette perte de fertilité est suffisamment substantielle pour rendre impraticable l'utilisation routinière de la semence cryoconservée. Les objectifs de notre laboratoire portent sur la caractérisation des dommages sous létaux subis par les spermatozoïdes lors de la cryoconservation et le développement de méthodes permettant d'améliorer la fertilité des spermatozoïdes décongelés. Cette revue décrit notre théorie selon laquelle les spermatozoïdes seraient prématurément activés par la cryoconservation ("cryo-capacitation"). Différentes stratégies permettant de prévenir ce dommage y sont également abordées.

show abstract

“…In some species, such as sheep, seminal plasma has been found to be beneficial, and removing seminal plasma from ram sperm causes a rapid decline in sperm viability (Ashworth et al 1994). The effect of seminal plasma is also dependent on diluents and extenders used (Maxwell and Johnson, 1999).…”

Section: Discussionmentioning

confidence: 99%

“…Mann (1964) extensively described the 'dilution effect' where sperm survival is greatly impaired by dilution from the initial ejaculate concentration when diluted in a simple saline solution. With the extenders/diluents used currently, this effect has been greatly reduced and allows for survival of sperm at concentrations of <10 6 sperm per ml for hours between sex-sorting and cryopreservation (Maxwell and Johnson, 1999). However, dilution may still be an issue.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Removing Seminal Plasma Improves Sex-Sorting of Bovine Sperm.

Burroughs

Graham

Lenz

et al. 2011

Biology of Reproduction

View full text Add to dashboard Cite

REMOVING SEMINAL PLASMA IMPROVES SEX-SORTING OF BOVINE SPERMExperiments for this thesis evaluated how characteristics of bovine ejaculates affect efficiency of sorting X-from Y-chromosome bearing sperm by flow cytometry/cell sorting, and further, document that removal of seminal plasma improves efficacy of sexsorting bovine sperm. In Experiment I, ejaculates were collected by artificial vagina, two each from 10 bulls with an average of one hour between collections. Semen was centrifuged to separate sperm from seminal plasma, and then sperm were re-diluted to 160x10 6 sperm per ml with staining TALP (Tyrode's albumin, lactate, pyruvate) and 0, 5, 10 or 20% seminal plasma from the same ejaculate or reciprocally from first/second ejaculates. Following incubation with Hoechst 33342, sperm were sorted and analyzed with a flow cytometer/cell sorter. The % live-oriented sperm was higher for treatments with 0% seminal plasma (64.4%) than 5 (59.6%), 10 (59.0%) and 20% (57.8%) seminal plasma (p<0.01). The % live-oriented sperm was higher for second (63.0%) than first ejaculates (56.2%). Sort rate was higher for samples with 0% seminal plasma (p<0.05).The percentages of membrane-impaired sperm were lower for 0% (16.5%) than 5 (21.9%), 10 (23.6%) or 20% (23.4%) seminal plasma (p<0.003), and for second iii ejaculates (18.2%) compared to first ejaculates (25.9%). Whether seminal plasma was from first versus second ejaculates had no effect (p>0.1). Effects of seminal plasma originating from bulls different from those whose sperm were sorted was evaluated in Experiment II. Semen collection and initial analysis were performed as in Experiment I and for all successive experiments. Seminal plasma from ejaculates of 6 bulls (3 that sorted well; 3 that sorted poorly) used in Experiment I was used with sperm collected for Experiment II as well as seminal plasma from sperm donors. Sperm were stained with Hoechst 33342 as in Experiment I, but with 10% seminal plasma in staining TALP.Only seminal plasma from one bull resulted in differences for any sorting parameters; 31% of sperm were membrane-impaired with seminal plasma from this bull compared to 16-19% for seminal plasma from other bulls (p<0.05). Sort rate was decreased (3.02x10 3 sperm per sec) compared to a range of 3.64-3.97x10 3 sperm per sec with the other seminal plasmas (p<0.05). This may have been due to contamination of the original semen sample, since this effect was only seen with one of six bulls.Experiment III investigated effects of adding bovine serum albumin (BSA) during staining with Hoechst 33342 using semen from 10 bulls. BSA was added at 0, 0.3 or 0.9% to staining TALP with either 0 or 10% seminal plasma. Although BSA has been shown to be beneficial to sperm, there was a possibility that BSA would bind Hoechst 33342, thereby reducing sorting efficiency. There was no evidence of either effect; however, sperm with 0% seminal plasma had higher % live oriented cells (65%) than sperm incubated with 10% seminal plasma (61%; p<0.05). In addition, samples conta...

show abstract

Physiology of spermatozoa at high dilution rates: the influence of seminal plasma

Cited by 224 publications

References 32 publications

Seminal Plasma Proteins as Potential Markers of Relative Fertility in Boars

Seminal Plasma Proteins as Potential Markers of Relative Fertility in Boars

Semen cryopreservation: Successes and persistent problems in farm species

Removing Seminal Plasma Improves Sex-Sorting of Bovine Sperm.

Contact Info

Product

Resources

About