Phytochemical analysis and biological activity of Corchorus olitorius L.: Quantitative analysis of bioactive compounds by LC–MS/MS, antibacterial, enzyme inhibition, and cytotoxic activities

Atalar, Mehmet Nuri; Erenler, Ram­azan; Türkan, Fikret; Alma, Mehmet Hakkı; Demirtaş, İbrahim; Baran, Ayşe; Kandemir, Sevgi İrtegün; Kekec, Ayse Ilgın; Saltan, Fatma Zerrin

doi:10.1016/j.eujim.2023.102290

Cited by 11 publications

(9 citation statements)

References 38 publications

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“…Therefore, the IC50 of MDA-MB-231 is >100 µg mL −1 ; meanwhile, the IC50 for Huh-7 is >1000 µg mL −1 ( Figure 2 ). Accordingly, the results reported here agree with those previously reported regarding the highly antiproliferative activity of C. Olitorius leaf extract against Caco-2 cell lines and had a low effect on sk-ov-3 cancer cell lines after treatment at the same concentration (250 µg mL −1 ) [ 6 ]. In another study, it was stated that the compounds in the Ocimum basilicum and Impatiens walleriana extracts suppressed cell viability by more than 78% [ 57 ].…”

Section: Resultssupporting

confidence: 92%

“…Plants have given humans and other organisms food and other health benefits for as long as they have existed [ 1 ]. Secondary metabolites, or bioactive chemicals, are present in medicinal plants [ 2 , 3 , 4 , 5 , 6 ]. Secondary metabolites are a class of substances that have been shown to have therapeutic action against several human disorders.…”

Section: Introductionmentioning

confidence: 99%

“…Quantitative assessments of the bioactive compounds in medicinal plants are essential in identifying the matching chemicals in particular plants. Thus, separating these chemicals at large concentrations from the relevant plants is possible [ 6 , 7 , 8 ]. Particularly in cancer therapy, scientific methods are used to devise treatments for patients.…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, this plant contains cardiac glycosides, phenolic acids, polysaccharides, sterols, and fatty acids, etc. [ 6 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

“…Patients may experience adverse effects from synthetic pharmaceuticals, however. For the treatment of diseases including cancer, Alzheimer's, and diabetes, therefore, the development and clinical application of Molecules 2024, 29, 1377 2 of 14 pharmaceuticals derived from plant materials are crucial [6,[9][10][11]. According to reports, roughly 80% of the global population derives its primary medication from natural products and plant-based medicines [12].…”

Section: Introductionmentioning

confidence: 99%

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Unveiling the Chemical Composition, Bioactive Profile and Antioxidant Capacity of Dried Egyptian Jew’s Mallow Stems as a Promising Anticancer Agent

Ali,

Ibrahim,

Salah-Eldin

2024

Molecules

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Phytochemicals from waste materials generated by agricultural and industrial processes have become globally significant due to their accessibility and potential effectiveness with few side effects. These compounds have essential implications in both medicine and the economy. Therefore, a quantitative analysis of the phytochemical profile, sugar types, and water-soluble vitamins of dried Corchorus olitorius L.“DJMS” extract (dried Jew’s mallow stem) was carried out with HPLC. In addition, the chemical composition, TPC, chlorophyll a and b, beta-carotene, and antioxidant effect using DPPH were investigated. Furthermore, the anticancer activity of the DJMS was evaluated by SRB assay using Huh-7 and MDA-MB-231 cell lines. In the quantitative study, DJMS extract showed a high antioxidant potential (67%) due to its content of bioactive compounds such as TPC (276.37 mg 100 g−1) and chlorophyll a and b (20.31, 12.02 mg 100 g−1, respectively), as well as some vitamins and minerals such as B-complex (B12; 146.8 mg 100 g−1 and vitamin C 6.49 mg 100 g−1) and selenium (<0.2 μg kg−1). Moreover, the main sugar types found were sucrose and stachyose, which recorded 9.23 and 6.25 mg 100 g−1, respectively. Identifying phenolic and flavonoids showed that the major components were ellagic acid (4905.26 μg kg−1), ferulic acid (3628.29 μg kg−1), chlorogenic acid (3757.08 μg kg−1), luteolin—7-O-glucoside (4314.48 μg kg−1), naringin (4296.94 μg kg−1) and apigenin—6—rhamnose—8 glucoside (3078.87 μg kg−1). The dried stem extract showed significant MDA-MB-231 inhibition activity and reached 80% at a concentration of 1000 µg/mL of DJMS extract, related to the content of phytochemical components such as isoflavones like genistein (34.96 μg kg−1), which had a tremendous anticancer effect. Hence, the stem of Jew’s mallow (which is edible and characterized by its viability and low production cost) possesses the capacity to serve as a pharmaceutical agent for combating cancer owing to its abundance of bioactive components.

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Section: Resultssupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Furthermore, this plant contains cardiac glycosides, phenolic acids, polysaccharides, sterols, and fatty acids, etc. [ 6 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Unveiling the Chemical Composition, Bioactive Profile and Antioxidant Capacity of Dried Egyptian Jew’s Mallow Stems as a Promising Anticancer Agent

Ali,

Ibrahim,

Salah-Eldin

2024

Molecules

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Arisarum vulgare: Bridging Tradition and Science Through Phytochemical Characterization and Exploring Therapeutic Potential via In vitro, In vivo, and In silico Biological Activities

Bouafia,

Boudjelal,

Bouaziz‐Terrachet

et al. 2024

ChemistrySelect

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Arisarum vulgare O. Targ. Tozz. (Araceae), locally known as “Elbgouga,” holds significant traditional importance in Algeria for the treatment of various human ailments, including pain, infections, inflammation, digestive disorders, cancer, skin problems, eczema, wounds, and burns. The aim of this study was to explore for the first time the phytochemical profile, antioxidant, antibacterial, and inhibitory effects on acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes, DNA protection, and capacity to promote wound healing. Preliminary phytochemical experiments were conducted to evaluate the major classes of bioactive compounds, in addition to the total phenol and flavonoid amounts in AVEE. An LC‐MS/MS analysis was conducted to clarify the phytochemical composition of this particular botanical species. The antioxidant capacity was assessed using DPPH· and ABTS·+ radical scavenging tests. The agar diffusion approach was used to ascertain the antibacterial efficacy against four bacteria (Staphylococcus aureus, Enterococcus faecium, Escherichia coli, and Salmonella typhimurium). Cholinesterase inhibition was evaluated using a colorimetric method that relies on Ellman's reaction. The protective effects of AVEE on pBR322 plasmid DNA damaged by H2O2 and UV treatment were assessed by their DNA‐breaking forms. The in vivo acute dermal toxicity and wound healing potential of the AVEE ointment (1–5% AVEO) were also investigated, and histological analyses were carried out on biological samples. Five protein targets were the subject of an in silico molecular docking investigation (TNFα, TGFBR1, IL‐1ß, GSK‐3ß, and NOS) and ADMET studies of the main components of the extracts were performed. The screening of phytochemicals revealed a significant concentration of phenolic compounds (176.00 ± 1.08 mg of gallic acid equivalents/g of dry extract), mainly flavonoids (81.21 ± 1.24 mg of quercetin equivalents/g of dry extract). Three major compounds were identified by LC‐MS/MS analysis, belonging mainly to the class of flavonoids, with rutin as the most abundant compound (11.85 mg/g extract), followed by hesperidin (4.125 mg/g extract). A conspicuous presence of isoquercitrin (1.327 mg/g extract) was also found. Small amounts of flavonoids and phenolic acids were also detected (catechin, chlorogenic acid, syringic acid, salicylic acid, kaempferol, and luteolin). DPPH assay showed higher antioxidant capacity compared to the ABTS·+ assay (142.60 ± 5.52 µg/mL and 236.10 ± 0.22 µg/mL, respectively). AVEE was effective against all selected bacterial strains; however, the highest zone of inhibition (36.00 ± 0.1 mm) was noted against E. faecium. The extract of the plant significantly inhibited both AChE and BChE (89.98 ± 18.76 µg/mL inhibitory activity against AChE and 98.28 ± 44.68 µg/mL inhibitory activity against BChE, respectively). The ethanolic extract of A. vulgare and quercetin exhibited more significant DNA protection action in form I, with percentages of 90.41 and 94.23%, respectively, compared to form II, where the percentages were 27.91 and 51.92%, respectively. The AVEO formulation may be safely administered by topical application. A statistically significant wound contraction was discovered in the group treated with 5% AVEO compared to the groups that were not treated or treated with petroleum jelly. Furthermore, there was no notable disparity detected between the group treated with 5% AVEO and the group treated with the reference drug. The 5% AVEO‐treated group showed, after the research was complete, the most significant percentage of wound contraction (96.90 ± 0.42%). Moreover, the topical application of the formulation improved histological parameters. In silico study showed that rutin, hesperidin, and isoquercitrin had a high affinity to the five main targets that might contribute to the wound healing potential of A. vulgare ethanolic extract. The impressive biological capabilities of A. vulgare indicate that the plant has the ability to be a valuable source of bioactive chemicals with various medical applications.

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Ziziphus spina-christi L. extract attenuates bleomycin-induced lung fibrosis in mice via regulating TGF-β1/SMAD pathway: LC-MS/MS Metabolic profiling, chemical composition, and histology studies

Elhady,

Goda,

Mehanna

et al. 2024

Biomedicine & Pharmacotherapy

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Phytochemical analysis and biological activity of Corchorus olitorius L.: Quantitative analysis of bioactive compounds by LC–MS/MS, antibacterial, enzyme inhibition, and cytotoxic activities

Cited by 11 publications

References 38 publications

Unveiling the Chemical Composition, Bioactive Profile and Antioxidant Capacity of Dried Egyptian Jew’s Mallow Stems as a Promising Anticancer Agent

Unveiling the Chemical Composition, Bioactive Profile and Antioxidant Capacity of Dried Egyptian Jew’s Mallow Stems as a Promising Anticancer Agent

Arisarum vulgare: Bridging Tradition and Science Through Phytochemical Characterization and Exploring Therapeutic Potential via In vitro, In vivo, and In silico Biological Activities

Ziziphus spina-christi L. extract attenuates bleomycin-induced lung fibrosis in mice via regulating TGF-β1/SMAD pathway: LC-MS/MS Metabolic profiling, chemical composition, and histology studies

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