Phytochemical Analysis and Chemical Characterization of Extracts and Blended Mixture of Palm Oil Leaf

Onakurhefe, P.; Achuba, F. I.; George, Betty

doi:10.26538/tjnpr/v3i9.2

Cited by 3 publications

(1 citation statement)

References 22 publications

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“…Previous studies have demonstrated that organic solvents with varying polarities, including ethanol, n-butanol, acetone, ethyl acetate, n-hexane, and petroleum, effectively retain alkaloids. [62][63][64][65][66] As most alkaloids have medium polarity, 29 solvents like ethanol (used in the soxhlet method) and acidic ethyl acetate solution (used in the maceration B method) are ideal for extracting alkaloids, penetrating deeper and releasing more extractable compounds. This explains the higher yields obtained for soxhlet and maceration B methods, which may also explain the low values obtained by maceration A and refluxing methods, since a high polarity organic solvent as methanol was applied.…”

Section: Alkaloids Detectionmentioning

confidence: 99%

Alkaloids from Marrubium vulgare L.: Antioxidant and Anti-Inflammatory Activities as a Function of Extraction Methods

2023

TJNPR

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The main objective is to determine the most suitable extraction technique for maximizing alkaloid yield and unlocking their potent biological activities. Extraction parameters, including yield, antioxidant activity, and anti-inflammatory activity, will be meticulously assessed. This study's findings will contribute to understanding the alkaloid profile of Marrubium vulgare L leaves and facilitate the development of effective extraction techniques for obtaining high-quality alkaloidrich extracts. Material and Method Chemicals and plant materialThe glassware used was purchased from Pyrex. Solvents (methanol, ethanol, hexane, chloroform, ethyl acetate, and diethyl ether) were purchased from Honeywell Fluka. Chemicals and reagents (Mayer's reagent, iron chloride, sodium hydroxide, ammonia, hydrochloric acid, sulfuric acid, sodium bicarbonate, sodium phosphate, ammonium molybdate, ammonium hydroxide, potassium ferricyanide, trichloroacetic acid, ascorbic acid, lithium chloride, Gel filtration for chromatography Sephadex G-50, aspirin, and formol) were purchased from Sigma-Aldrich (Casablanca, Morocco). Extracts concentration was done using a rotary evaporator (BUCHI, Rotavapor R-100, Germany), and optical density measurements were performed on Ultraviolet/Visible spectrophotometer (Shimadzu UV-1600 PC UV, Japan). Molecular structures and physicochemical data were obtained from PubChem and SwissADME programs (https://pubchem.ncbi.nlm.nih.gov/, http://www.swissadme.ch/). Plant material used consists of Marrubium vulgare L leaves, which were collected during February and March 2021 in the Fez-Meknes region and were identified by Dr. Chaimae Rais in The National Agency for Medicinal and Aromatic Plants, Taounate, Morocco (Document no. 075/2021). The study was conducted at Human Pathology, Biomedicine and Environment Laboratory, in the Faculty of Medicine and Pharmacy (34.00612274141898, -4.964672169974484).

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Section: Alkaloids Detectionmentioning

confidence: 99%

Alkaloids from Marrubium vulgare L.: Antioxidant and Anti-Inflammatory Activities as a Function of Extraction Methods

2023

TJNPR

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Effect of Elaeis guineensis (Jacq) leaf extracts on crude oil-induced genotoxicity in Wistar albino rats

et al. 2020

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Preliminary toxicology profile of Dennettia tripetala (Pepper Fruit) methanolic leaves extract

et al. 2021

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Background Dennentia tripetalla (Pepper Fruit) belongs to the Annonaceae family and is abundant in Nigeria. Its fruit in folklore medicine is used for treatment of varying ailments. While ample research evidence exists on the plants fruit and seed, no current study exists on the toxicological profile of the plant leaves. Methods qualitative and quantitative phytochemicals and In vitro antioxidant assays were carried out using standard methods. The acute toxicity study indicates that the LD50 was higher than 2000 mg/Kg body weight. Sub-chronic toxicity studies was carried out using five groups of rats. Group 1 served as control, 2–5 received 100 mg/Kg, 200 mg/Kg, 500 mg/Kg and 1000 mg/Kg body weight orally for 28 days. Results Post-administration biochemical analysis indicates there was increased weight in rats administered 100 mg/kg and 200 mg/kg while it reduced in the 500 mg/kg group. Significant elevations of liver function markers were reported for 200 mg/kg and 500 mg/kg respectively. Serum and hepatic protein profiles remained unaltered. Renal function analysis revealed elevated serum urea and creatinine for 200 and 500 mg/kg groups, elevated serum Na+ and Ca+ and reduced serum Cl− for the 500 mg/Kg group. Elevated Kidney K+ and Ca+ levels, reduced Cl− were significantly observed in 500 mg/Kg group. Significant rise in hepatic and renal lipid peroxidation was observed in 200 and 500 mg/Kg groups. There were observed disarmament of the antioxidant defense systems occasioned by rise and drop in tissue (hepatic, renal, testes, heart) Superoxide dismutase (SOD), Catalase (Cat), Glutathione-s-transferase (GST), Glutathione peroxidase (GPx) activities in the test groups relative to control. Histopathological examination indicated architectural aberrations at 500 and 1000 mg/kg. Conclusions It concluded that the plant had significant phytochemical and antioxidant properties of medical interest and possessed toxic properties in rats when administered at a dose above 200 mg/Kg over a prolonged period of time.

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Phytochemical Analysis and Chemical Characterization of Extracts and Blended Mixture of Palm Oil Leaf

Cited by 3 publications

References 22 publications

Alkaloids from Marrubium vulgare L.: Antioxidant and Anti-Inflammatory Activities as a Function of Extraction Methods

Alkaloids from Marrubium vulgare L.: Antioxidant and Anti-Inflammatory Activities as a Function of Extraction Methods

Effect of Elaeis guineensis (Jacq) leaf extracts on crude oil-induced genotoxicity in Wistar albino rats

Preliminary toxicology profile of Dennettia tripetala (Pepper Fruit) methanolic leaves extract

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