Phytochemical analysis and in vitro cytostatic potential of ethnopharmacological important medicinal plants

Verma, Akalesh Kumar; Singh, Sweta

doi:10.1016/j.toxrep.2020.02.016

Cited by 61 publications

(24 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We observed a reduction in Δψm, indicating that that MN may operate on mitochondria, inducing Δψm depletion and subsequent apoptosis, probably via ROS. These results were in good agreement with the earlier studies carried out with Murraya koenigii extracts in Dalton's Ascites Lymphoma (DL) cell line [47], isolated carbazole alkaloids in HL-60 cells [48], and Koenimbine in HepG2 cells [49]. These findings together with our results suggest that this plant and its alkaloids induced cell death via mitochondria involvement.…”

Section: Discussionsupporting

confidence: 93%

Cytotoxicity of Mahanimbine from Curry Leaves in Human Breast Cancer Cells (MCF-7) via Mitochondrial Apoptosis and Anti-Angiogenesis

Hobani

2022

Molecules

View full text Add to dashboard Cite

Mahanimbine (MN) is a carbazole alkaloid present in the leaves of Murraya koenigii, which is an integral part of medicinal and culinary practices in Asia. In the present study, the anticancer, apoptotic and anti-invasive potential of MN has been delineated in vitro. Apoptosis cells determination was carried out utilizing the acridine orange/propidium iodide double fluorescence test. During treatment, caspase-3/7,-8, and-9 enzymes and mitochondrial membrane potentials (Δψm) were evaluated. Anti-invasive properties were tested utilizing a wound-healing scratch test. Protein and gene expression studies were used to measure Bax, Bcl2, MMP-2, and -9 levels. The results show that MN could induce apoptosis in MCF-7 cells at 14 µM concentration IC50. MN-induced mitochondria-mediated apoptosis, with loss in Δψm, regulation of Bcl2/Bax, and accumulation of ROS (p ≤ 0.05). Caspase-3/7 and -9 enzyme activity were detected in MCF-7 cells after 24 and 48 h of treatment with MN. The anti-invasive property of MN was shown by inhibition of wound healing at the dose-dependent level and significantly suppressed mRNA and protein expression on MMP-2 and -9 in MCF-7 cells treated with a sub-cytotoxic dose of MN. The overall results indicate MN is a potential therapeutic compound against breast cancer as an apoptosis inducer and anti-invasive agent.

show abstract

Section: Discussionsupporting

confidence: 93%

Cytotoxicity of Mahanimbine from Curry Leaves in Human Breast Cancer Cells (MCF-7) via Mitochondrial Apoptosis and Anti-Angiogenesis

Hobani

2022

Molecules

View full text Add to dashboard Cite

show abstract

“…In this context, medicinal plants play a valuable investment role due to their bioactive compounds [ 73 ]. Recent works have documented the phytochemical importance of medicinal plants and their bioactive compounds [ 74 , 75 , 76 , 77 ]. The biological characteristics of medicinal plants play a pivotal role in the characterization of NPs [ 78 , 79 ].…”

Section: Discussionmentioning

confidence: 99%

Environmentally Safe Biosynthesis of Gold Nanoparticles Using Plant Water Extracts

et al. 2021

View full text Add to dashboard Cite

Due to their simplicity of synthesis, stability, and functionalization, low toxicity, and ease of detection, gold nanoparticles (AuNPs) are a natural choice for biomedical applications. AuNPs’ unique optoelectronic features have subsequently been investigated and used in high-tech applications such as organic photovoltaics, sensory probes, therapeutic agents, the administration of drugs in biological and medical applications, electronic devices, catalysis, etc. Researchers have demonstrated the biosynthesis of AuNPs using plants. The present study evaluates 109 plant species used in the traditional medicine of Middle East countries as new sources of AuNPs in a wide variety of laboratory environments. In this study, dried samples of bark, bulb, flower, fruit, gum, leaf, petiole, rhizome, root, seed, stamen, and above-ground parts were evaluated in water extracts. About 117 plant parts were screened from 109 species in 54 plant families, with 102 extracts demonstrating a bioreduction of Au3+ to Au0, revealing 37 new plant species in this regard. The color change of biosynthesized AuNPs to gray, violet, or red was confirmed by UV-Visible spectroscopy, TEM, FSEM, DLS, and EDAX of six plants. In this study, AuNPs of various sizes were measured from 27 to 107 nm. This study also includes an evaluation of the potency of traditional East Asian medicinal plants used in this biosynthesis of AuNPs. An environmentally safe procedure such as this could act as a foundation for cosmetic industries whose quality assessment systems give a high priority to non-chemically synthesized products. It is crucial that future optimizations are adequately documented to scale up the described process.

show abstract

“…The cationic fluorescent dye rhodamine 123 (Rh123) for determination of mitochondrial membrane potential [ 55 ] was used as Rh123, via facilitated diffusion, selectively accumulates in the mitochondria [ 56 ]. However, there is an indirect relationship between mitochondrial potential and the amount of Rh123 that enters the mitochondria.…”

Section: Methodsmentioning

confidence: 99%

Resveratrol, curcumin and gallic acid attenuate glyoxal-induced damage to rat renal cells

et al. 2020

View full text Add to dashboard Cite

show abstract

Phytochemical analysis and in vitro cytostatic potential of ethnopharmacological important medicinal plants

Cited by 61 publications

References 32 publications

Cytotoxicity of Mahanimbine from Curry Leaves in Human Breast Cancer Cells (MCF-7) via Mitochondrial Apoptosis and Anti-Angiogenesis

Cytotoxicity of Mahanimbine from Curry Leaves in Human Breast Cancer Cells (MCF-7) via Mitochondrial Apoptosis and Anti-Angiogenesis

Environmentally Safe Biosynthesis of Gold Nanoparticles Using Plant Water Extracts

Resveratrol, curcumin and gallic acid attenuate glyoxal-induced damage to rat renal cells

Contact Info

Product

Resources

About