Phytochemical analysis and in vitro cytotoxicity test of black soybean (<i>Glycine soja</i>L.) ethanolic extract as a growth inhibitor of the HCT-116 colon carcinoma cell line

Hidayat, DA; Dwira, Surya

doi:10.1088/1742-6596/1073/3/032041

Cited by 10 publications

(12 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This facilitates assessment of their survival rate, and thus the assessment of the action of cytotoxic compounds [21]. In the case of damaged or dead cells, formazan is generated in lower amounts or is not formed at all [22].…”

Section: Mtt Cytotoxicity Testmentioning

confidence: 99%

Phenolic Compound Composition and Biological Activities of Fractionated Soybean Pod Extract

Pabich¹,

Marciniak²,

Kontek³

2021

Applied Sciences

View full text Add to dashboard Cite

The objective of this study was to determine the potential of dry extracts from soybean pods as a source of bioactive compounds. The phenolic compound composition of the extract (E) and fraction (F1-F3) as well as their respective lipophilicity were analyzed by UPLC-ESI-QTOF-MS. The biological activity as measured by antioxidant and cytotoxic activity was also evaluated. The in vitro anticancer potential of the ethanolic extract and fraction of soybean pods was measured through MTT assay using human colorectal carcinoma (HTC-116) and prostate cancer (PC-3) cell lines. The F2 fraction, with medium lipophilicity, produced the most pronounced cytotoxic effect in PC-3 cells. Analysis of Pearson’s correlation demonstrated a high relationship between the content of phenolic compounds, the antioxidant activity of the extract and fractions, and cytotoxic activity towards PC-3 cells. UPLC-ESI-QTOF-MS analysis of soybean pod extract resulted in putative identification of 50 polyphenols belonging to three different chemical families (phenolic acids, flavonoids, and other polyphenols), the first time such an analysis has been performed. Among the fractions with variable lipophilicity, fraction F2 was the most abundant in phenolic compounds. This was confirmed in the quantitative analysis, as a result of which the highest total content of phenolic compounds was found in the extract (E) and fraction F2. Soybean pods might be useful material for obtaining preparations with anti-radical and anti-cancer properties. They can also be an active food additive or a component in dietary supplements.

show abstract

Section: Mtt Cytotoxicity Testmentioning

confidence: 99%

Phenolic Compound Composition and Biological Activities of Fractionated Soybean Pod Extract

Pabich¹,

Marciniak²,

Kontek³

2021

Applied Sciences

View full text Add to dashboard Cite

show abstract

“…Therefore, CAE shows moderate cytotoxicity—IC 50 values (µg/mL) < 200 µg/mL—on LoVo and MDA-MB-231 cells after 48 h of exposure, and a low one—IC 50 = 200–400 µg/mL—on the same cells after 24 h of exposure. On PE/CA-PJ49, CAE exhibits moderate cytotoxic activity after 24 and 48 h; the same effect is recorded on SK-OV-3 after 48 h [ 17 ]. Finally, CAE has no cytotoxicity—IC 50 >> 400 µg/mL—on HEP G2, HT-29, and HUVECs after 24 and 48 h and on SK-OV-3 after 24 h [ 17 ].…”

Section: Resultsmentioning

confidence: 95%

“…The IC 50 values displayed in Table 4 could be interpreted according to Hidayat et al [ 17 ], resulting in an overview of CAE cytotoxic activity on various tested cell lines. Therefore, CAE shows moderate cytotoxicity—IC 50 values (µg/mL) < 200 µg/mL—on LoVo and MDA-MB-231 cells after 48 h of exposure, and a low one—IC 50 = 200–400 µg/mL—on the same cells after 24 h of exposure.…”

Section: Resultsmentioning

confidence: 99%

Phytochemical Profile, Antioxidant and Cytotoxic Potential of Capsicum annuum (L.) Dry Hydro-Ethanolic Extract

Ivan,

Popovici,

Chițescu

et al. 2024

Pharmaceutics

View full text Add to dashboard Cite

Capsicum annuum (L.) is one of the essential spices most frequently used in our daily routine and has remarkable ethnobotanical and pharmacological properties. Its fruits are rich in vitamins, minerals, carotenoids, and numerous other phenolic metabolites with a well-known antioxidant activity. Regular consumption of chili fruits may have a positive influence on human health. Therefore, we investigated a commercially available chili fruit powder in the present study, extracting it with 50% ethanol. The dried hydro-ethanolic extract (CAE) was thoroughly analyzed using ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry (UHPLC–HRMS/MS), and 79 bioactive phenolic constituents were identified. Then, we quantified the main phenolic compounds and found a polyphenol content of 4.725 ± 1.361 mg Eq tannic acid/100 g extract and a flavonoid amount of 1.154 ± 0.044 mg Eq rutin/100 g extract. Phenolic secondary metabolites are known for their dual redox behavior as antioxidants/pro-oxidants, underlying their numerous benefits in health and disease. Thus, the antioxidant potential of CAE was evaluated using three methods; our results could explain the protective effects of chili fruits: IC50DPPH = 1.669 mg/mL, IC50ABTS = 0.200 mg/mL, and EC50FRAP = 0.561 mg/mL. The pro-oxidant potential of phenolic compounds could be a basis for CAE cytotoxicity, investigated in vitro on tumor cell lines and in vivo on Daphnia sp. Results demonstrated the dose- and time-dependent CAE’s cytotoxic activity; the highest antiproliferative activity was recorded on colon (LoVo) and breast (MDA-MB-231) cancer cell lines after 48 h of exposure (IC50 values < 200 µg/mL). In vivo testing on Daphnia sp. reported a potent CAE cytotoxicity after 48 h and embryonic developmental delays. Extensive data analyses support our results, showing a significant correlation between the CAE’s concentration, phenolic compound content, antioxidant activity, exposure time, and the viability rate of different tested cell lines.

show abstract

“…10 determined IC50 values of 100-250 µg/mL for individual fractions of wild soybean extract against MCF-7 cells. MTT assay of wild soybean extract on HCT-116 cells exhibited IC50 value of 97.56 µg/mL 11 . In an in vitro study, Wang,et.…”

Section: Resultsmentioning

confidence: 97%

In Vitro Cytotoxic Activity of Three Egyptian Soybean Cultivars

Sattar¹,

Monem²,

Hegazy³

et al. 2019

Int. Res. J. Pharm.

View full text Add to dashboard Cite

Functional foods play a key role in traditional indigenous medical system that have continued to cure many diseases in developing countries. In order to find new potent source of bioactive principles, the present study was designed to evaluate the cytotoxic effect of the defatted extract from soybean flour of three Egyptian soybean cultivars (Giza 22, Giza 35 and Giza 111). Subsequently, MTT (Methylthiazolyl diphenyl-tetrazolium bromide) assay was used to determine IC50 (half-maximal inhibitory concentration compared with control) of the different extracts against six cancer cell lines, namely HCT-116, EAC, HepG2, CCRF-CEM, PC-3 and MCF-7. The extracts of the three cultivars demonstrated IC50 at values less than 100 µg/mL against all the tested cell lines except CCRF-CEM. Among the studied cell lines, IC50 values below 20 µg/mL were recorded for all extracts against EAC cells. Giza 35 and Giza 111 extracts showed good cytotoxicity against HCT-116 and MCF-7 cells, respectively. Finally, these results provided corroborative information for the possible use of soybean flour as a natural source in the control of cancer disease.

show abstract

Phytochemical analysis and in vitro cytotoxicity test of black soybean (Glycine sojaL.) ethanolic extract as a growth inhibitor of the HCT-116 colon carcinoma cell line

Cited by 10 publications

References 6 publications

Phenolic Compound Composition and Biological Activities of Fractionated Soybean Pod Extract

Phenolic Compound Composition and Biological Activities of Fractionated Soybean Pod Extract

Phytochemical Profile, Antioxidant and Cytotoxic Potential of Capsicum annuum (L.) Dry Hydro-Ethanolic Extract

In Vitro Cytotoxic Activity of Three Egyptian Soybean Cultivars

Contact Info

Product

Resources

About