Phytochemical analysis and in vitro biological activity of three Hypericum species from the Canary Islands (Hypericum reflexum, Hypericum canariense and Hypericum grandifolium)

Zorzetto, Christian; Sánchez-Mateo, Candelaria C.; Rabanal, Rosa M.; Lupidi, Giulio; Petrelli, Dezemona; Vitali, Luca A.; Bramucci, Massimo; Quassinti, Luana; Caprioli, Giovanni; Papa, Fabrizio; Ricciutelli, Massimo; Sagratini, Gianni; Vittori, Sauro; Maggi, Filippo

doi:10.1016/j.fitote.2014.11.013

Cited by 65 publications

(59 citation statements)

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“…Furthermore, the flavonoids hyperoside and isoquercitrin are listed together in the results as it is known that certain Hypericum spp. might also contain isoquercitrin (Zorzetto et al, 2015); unfortunately, hyperoside and isoquercitrin could not be distinguished by means of MALDI-HRMS or MALDI-HRMS/MS.…”

Section: Resultsmentioning

confidence: 99%

“…The majority of morphological and phytochemical studies have so far been realized on one well-known species, Hypericum perforatum , commonly known as St. John's wort. A broad spectra of secondary metabolites with diverse biological activities have been found in this species, for example, the naphthodianthrone derivatives hypericin and pseudohypericin, their supposed precursor emodin, and/or emodin anthrone (Bais et al, 2003; Kirakosyan et al, 2004; Zobayed et al, 2006), phloroglucinols hyperforin, adhyperforin and flavonoids quercetin, quercitrin, rutin and hyperoside and/or isoquercitrin, to name a few (Nahrstedt and Butterweck, 1997; Saddiqe et al, 2010; Zorzetto et al, 2015; Hou et al, 2016; Oliveira et al, 2016). Among these compounds, the effectiveness of hypericin as a natural photosensitizer with implications in photodynamic therapy has been rigorously explored (Zhang et al, 2014; Jendželovská et al, 2016), in addition to studies on its antiviral and antimicrobial properties (Kubin et al, 2005; Karioti and Bilia, 2010; Dementavicius et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Occurrence and Distribution of Phytochemicals in the Leaves of 17 In vitro Cultured Hypericum spp. Adapted to Outdoor Conditions

et al. 2016

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A plethora of plants belonging to the genus Hypericum have been investigated so far owing to the biological efficacies of pharmacologically important secondary metabolites produced by several Hypericum species. However, there is currently a dearth of information about the localization (accumulation) of these compounds in the plants in situ. In particular, the biosynthetic and ecological consequence of acclimatization of in vitro cultured Hypericum spp. to outdoor conditions is not fully known. Herein, we report an application of matrix-assisted laser desorption/ionization high-resolution mass spectrometry (MALDI-HRMS) to reveal the distribution of major naphthodianthrones hypericin, pseudohypericin, protohypericin, and their proposed precursor emodin as well as emodin anthrone, along with the phloroglucinol derivative hyperforin, the flavonoids quercetin, quercitrin, rutin and hyperoside (and/or isoquercitrin), and chlorogenic acid in Hypericum leaves. Plants encompassing seventeen Hypericum species classified into eleven sections, which were first cultured in vitro and later acclimatized to outdoor conditions, were studied. We focused both on the secretory (dark and translucent glands, other types of glands, and glandular-like structures) as well as the non-secretory leaf tissues. We comparatively analyzed and interpreted the occurrence and accumulation of our target compounds in different leaf tissues of the seventeen species to get an intra-sectional as well as inter-sectional perspective. The naphthodianthrones, along with emodin, were present in all species containing the dark glands. In selected species, hypericin and pseudohypericin accumulated not only in the dark glands, but also in translucent glands and non-secretory leaf tissues. Although hyperforin was localized mainly in translucent glands, it was present sporadically in the dark glands in selected species. The flavonoids quercetin, quercitrin, and hyperoside (and/or isoquercitrin) were distributed throughout the leaves. Rutin was present only within sections Hypericum, Adenosepalum, Ascyreia, and Psorophytum. Our study provides insights into the prospects and challenges of using in vitro cultured Hypericum plants, further adapted to field conditions, for commercial purposes.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Occurrence and Distribution of Phytochemicals in the Leaves of 17 In vitro Cultured Hypericum spp. Adapted to Outdoor Conditions

et al. 2016

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“…The ABTS radical scavenging capacity assay was conducted according to the procedures described by [18, 19]. …”

Section: Methodsmentioning

confidence: 99%

Cat’s whiskers (Orthosiphon stamineus) tea modulates arthritis pathogenesis via the angiogenesis and inflammatory cascade

Tabana¹,

Al‐Suede

Ahamed

et al. 2016

BMC Complement Altern Med

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Background Orthosiphon stamineus is used traditionally to treat gout, arthritis, and inflammatory related conditions. The in vitro anti-inflammatory effects of the plant have been scientifically investigated. The goal of the present study was to evaluate the potential of the 50% ethanol extract of O. stamineus (EOS) to treat rheumatoid arthritis.MethodsAnti-arthritic activity was assessed using the in vitro heat denaturation test and the (FCA)-induced arthritis model. Efficacy was assessed by measurements of paw edema and granulation, X-ray radiography, fluorescence molecular tomography (FMT), and histological evaluation. Levels of (TNF-α), interleukin-1 (IL-1), and (COX-1 and COX-2) were analyzed in vitro in lipopolysaccharide (LPS)-stimulated human macrophage (U937). TNF-α and IL-1 levels in the serum samples of arthritic rats were also measured using an ELISA kit.ResultsTreatment with EOS resulted in dose-dependent inhibition of paw edema in acute and chronic models of inflammation. It also inhibited significantly the production of TNF-α, IL-1 COX-1, and COX-2 in the LPS-stimulated U937 macrophages. EOS significantly suppressed FCA-induced paw edema as well as the serum levels of TNF-α and IL-1. X-rays of the synovial joint of the hind leg showed considerable improvement in joint integrity and recovery of tibia-talus bones from degeneration and osteoporotic lesions. Histology of proximal interphalangeal joints of EOS-treated animals showed obvious protection of cartilage and soft tissue. Finally, FMT analysis strongly supported the anti-arthritic effect of EOS. EOS had high phenolic and total flavonoid content as well as strong antioxidant activity.ConclusionsResults illustrated that the anti-arthritic properties of O. stamineus could be beneficial for prevention and management of rheumatoid arthritis and other chronic inflammatory disorders.Graphical abstractIllustration of the Anti- arthritis efficacy of Orthosiphon Stamineus standardized extract.

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“…In this work, we evaluated the free radical scavenging capacity of S. sericea Naviglio® extracts using multiple assays such as DPPH, ABTS + and FRAP (Zorzetto et al 2015a(Zorzetto et al , 2015b and results are presented in Table 2. In the DPPH assay, the IC 50 value of the reference compound Trolox amounted to 4.9 μg/mL ( Table 3).…”

Section: Antioxidant Activitymentioning

confidence: 99%

Phenolic acids, antioxidant and antiproliferative activities of Naviglio® extracts from Schizogyne sericea (Asteraceae)

Caprioli

Iannarelli

Sagratini

et al. 2016

Natural Product Research

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Schizogyne sericea, well-known as 'salado', is a halophytic shrub widespread on coastal rocks of Tenerife (Canary Islands). This plant is used traditionally as analgesic, astringent, anti-inflammatory and vulnerary agent. In the present work, we have analysed the aqueous and ethanolic extracts of S. sericea for the content of phenolic acids by HPLC-DAD. The dynamic solid-liquid Naviglio® extractor was used to extract the flowering aerial parts. Aqueous extracts showed higher levels of phenolics than ethanolic extracts. S. sericea extracts were rich in chlorogenic and isochlorogenic acids. The Naviglio® extracts obtained were assayed for in vitro biological activities, namely antioxidant, antimicrobial and cytotoxicity on tumour cells by DPPH, ABTS, FRAP, agar disc-diffusion and MTT methods, respectively. Results showed that aqueous extracts, being richer in phenolic acids, are endowed with relevant radical scavenging activity (TEAC values in the range 208-960 μmol TE/g) while ethanolic extracts exhibited noteworthy antiproliferative effects on tumour cells.

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Phytochemical analysis and in vitro biological activity of three Hypericum species from the Canary Islands (Hypericum reflexum, Hypericum canariense and Hypericum grandifolium)

Cited by 65 publications

References 59 publications

Occurrence and Distribution of Phytochemicals in the Leaves of 17 In vitro Cultured Hypericum spp. Adapted to Outdoor Conditions

Occurrence and Distribution of Phytochemicals in the Leaves of 17 In vitro Cultured Hypericum spp. Adapted to Outdoor Conditions

Cat’s whiskers (Orthosiphon stamineus) tea modulates arthritis pathogenesis via the angiogenesis and inflammatory cascade

Phenolic acids, antioxidant and antiproliferative activities of Naviglio® extracts from Schizogyne sericea (Asteraceae)

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