Phytochemical Analysis and in Vitro Antioxidant Potential of Annona Muricata L.

Kumar, Rahul; R, Madhanraj; Illuri, Ramanaiah; Maya, Michelle; Venkatakrishna, K.; Rameshkumar, K. B.; Veeramanikandan,; Eyini, M.; Balaji, P.

doi:10.7897/2230-8407.1004131

Cited by 57 publications

(77 citation statements)

References 9 publications

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“…Unfortunately, even in the presence of only 10 % DMSO and 90 % CDCl 3 , signals from disaggregated Fmoc‐GC units are dominant in the spectra. In fact, in the 2D [ 1 H, 1 H] TOCSY experiments, the presence of four characteristic conformations for the monomeric GC PNA chain can be revealed (Figure S4A) and no clear NOE contacts between G and C could be found (Figure S4B) in the 2D [ 1 H, 1 H] NOESY spectrum . The presence of DMSO, the flexibility of the PNA backbone and the extensive line broadening of the aggregates with larger molecular weights, is surely influencing this outcome, hindering the observation of ordered aggregates in solution provided with canonical GC H‐bonds.…”

Section: Resultsmentioning

confidence: 99%

Self‐Assembling of Fmoc‐GC Peptide Nucleic Acid Dimers into Highly Fluorescent Aggregates

Avitabile

Diaferia

Ventura

et al. 2018

Chemistry A European J

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Self-assembling of molecules by non-covalent interactions is one of the most attracting topics in supramolecular chemistry. The use of short peptides or modified nucleotides as building blocks of the aggregates is particularly intriguing, as these are very easy to synthesize; moreover subtle changes in the chemical structure of such building blocks may drastically affect the properties of the aggregates. The ability of Peptide Nucleic Acids to aggregate is yet very little explored, despite its practical applications. In this work we investigated the self-assembling properties of a PNA dimer, conjugated at the N-terminus to a fluorenylmethoxycarbonyl group. This PNA dimer forms nano-aggregates at low concentration in CHCl 3 /CH 3 OH mixtures. The aggregates keep very interesting fluorescent properties (high quantum yield in the visible region with lifetime in the nanoseconds scale), which make them a promising material for applications in optoelectronic.

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Section: Resultsmentioning

confidence: 99%

Self‐Assembling of Fmoc‐GC Peptide Nucleic Acid Dimers into Highly Fluorescent Aggregates

Avitabile

Diaferia

Ventura

et al. 2018

Chemistry A European J

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“…In total, eight transparent ME systems with droplet sizes between 6.35 and 10.16 nm were obtained in absence or presence of PTX (Table ). These nanometric droplet sizes enable ME to be sterilized by filtration in the case of future medical applications …”

Section: Resultsmentioning

confidence: 81%

“…Microemulsions (ME) were discovered by Hoar and Schulman in 1943; however, the term “microemulsion” was introduced 16 years later by Jack Schulman and coworkers: “A microemulsion is a system of water, oil and amphiphile which is a single, optically isotropic and thermodynamically stable liquid solution” . ME have at least three components: a polar and a nonpolar liquid phase (water and oil, respectively) and a suitable surfactant frequently in combination with a co‐surfactant such as a short chain aliphatic alcohol .…”

Section: Introductionmentioning

confidence: 99%

Formation, Stability and Cytotoxicity of Precursor Microemulsions to Prepare Core‐Shell Polymeric Nanoparticles for Pharmaceutical Applications

Peralta

Flores-Villaseñor

Ramirez-Contreras

et al. 2017

Macromolecular Symposia

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The preparation of biocompatible normal (oil‐in‐water, o/w) microemulsions (ME) with different oil phases (peppermint oil, trans‐anethole, vitamin E, jojoba oil) and stabilized with d‐α polyethylene glycol succinate (TPGS‐1000) and isobutanol (iso‐BuOH) is presented. Results of average particle diameter determination, Dp, indicated 6.35 ≤ Dp ≤ 10.24 nm. These ME showed excellent colloidal stability (−40 and +40 °C) and, without any drug loaded, decreased dramatically the viability of leukemia cells (K562), 60–90 %, in 24 hours. Besides, this behavior was potentiated when paclitaxel (PTX, an anticancer drug) was loaded in the transanethole microemulsion, where the cell viability decreased until 2 % at 0.5 μg/mL of PTX. These MEs are precursors to synthetize polymeric nanocapsules when coated with a thermo or pH – sensible polymer with potential for cancer treatment.

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“…1D [ 1 H] proton experiments were recorded with a relaxation delay d1 = 1.5 s and 64 scans. 2D [ 1 H, 1 H] spectra were as well recorded and analyzed: TOCSY (Total Correlation Spectroscopy), NOESY (Nuclear Overhauser Enhancement Spectroscopy), and ROESY (Rotating Frame Overhauser Enhancement Spectroscopy). These sets of 2D experiments were generally measured with the following acquisition parameters: 16–64 scans, 128–256 FIDs in t1, 1,024 or 2,048 data points in t2, TOCSY mixing time equal to 70 ms, NOESY mixing time equal to 300 and ROESY mixing time equal to 250 ms. Water suppression was obtained with the DPFGSE (Double Pulsed Field Gradient Selective Echo) sequence.…”

Section: Methodsmentioning

confidence: 99%

Structure‐activity studies of peptidomimetics based on kinase‐inhibitory region of suppressors of cytokine signaling 1

et al. 2018

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Suppressors of Cytokine Signaling (SOCS) proteins are negative regulators of JAK proteins that are receptor-associated tyrosine kinases, which play key roles in the phosphorylation and subsequent activation of several transcription factors named STATs. Unlike the other SOCS proteins, SOCS1 and 3 show, in the N-terminal portion, a small kinase inhibitory region (KIR) involved in the inhibition of JAK kinases. Drug discovery processes of compounds based on KIR sequence demonstrated promising in functional in vitro and in inflammatory animal models and we recently developed a peptidomimetic called PS5, as lead compound. Here, we investigated the cellular ability of PS5 to mimic SOCS1 biological functions in vascular smooth muscle cells and simultaneously we set up a new binding assay for the screening and identification of JAK2 binders based on a SPR experiment that revealed more robust with respect to previous ELISAs. On this basis, we designed several peptidomimetics bearing new structural constraints that were analyzed in both affinities toward JAK2 and conformational features through Circular Dichroism and NMR spectroscopies. Introduced chemical modifications provided an enhancement of serum stabilities of new sequences that could aid the design of future mimetic molecules of SOCS1 as novel anti-inflammatory compounds.

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Phytochemical Analysis and in Vitro Antioxidant Potential of Annona Muricata L.

Cited by 57 publications

References 9 publications

Self‐Assembling of Fmoc‐GC Peptide Nucleic Acid Dimers into Highly Fluorescent Aggregates

Self‐Assembling of Fmoc‐GC Peptide Nucleic Acid Dimers into Highly Fluorescent Aggregates

Formation, Stability and Cytotoxicity of Precursor Microemulsions to Prepare Core‐Shell Polymeric Nanoparticles for Pharmaceutical Applications

Structure‐activity studies of peptidomimetics based on kinase‐inhibitory region of suppressors of cytokine signaling 1

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