Phytochemical Analysis, Antidiabetic Potential and in-silico Evaluation of Some Medicinal Plants

Abstract

Background:The increasing frequency of diabetes patients and the reported side effects of commercially available anti-hyperglycemic drugs have gathered the attention of researchers towards the search for new therapeutic approaches. Inhibition of activities of carbohydrate hydrolyzing enzymes is one of the approaches to reduce postprandial hyperglycemia by delaying digestion and absorption of carbohydrates. Objectives: The objective of the study was to investigate phytochemicals, antioxidants, digestive enzymes inhibitory effect, and molecular docking of potent extract. Materials and Methods: In this study, we carry out the substratebased α-glucosidase and α-amylase inhibitory activity of Asparagus racemosus, Bergenia ciliata, Calotropis gigantea, Mimosa pudica, Phyllanthus emblica, and Solanum nigrum along with the determination of total phenolic and flavonoids contents. Likewise, the antioxidant activity was evaluated by measuring the scavenging of DPPH radical. Additionally, antibacterial activity was also studied by Agar well diffusion method. Molecular docking of bioactive compounds from B. ciliata was performed via AutoDock vina. Results: B. ciliata, M. pudica, and P. emblica exhibit significant inhibitory activity against the α-glucosidase and α-amylase with IC 50 (µg/ml) of (2.24 ± 0.01, 46.19 ± 1.06), (35.73 ± 0.65, 99.93 ± 0.9) and (8.12 ± 0.29, no significant activity) respectively indicating a good source for isolating a potential drug candidate for diabetes. These plant extracts also showed significant antioxidant activity with the IC 50 ranges from 13.2 to 26.5 µg/mL along with the significant antibacterial activity towards Staphylococcus aureus and Klebsiella pneumonia. Conclusion: Bergenia extract appeared to be a potent α-glucosidase and α-amylase inhibitor. Further research should be carried out to characterize inhibitor compounds.

Biological activity and GC-MS determination of bioactive components of Clerodendrum viscosum (Vent.) from Siraha District, Nepal

“…The TFC was estimated using quercetin (10-100 μg/mL) calibration curve, and the results were represented as milligrams of quercetin equivalent per gram dry weight of extract (mg QE/g). To ensure the reproducibility of the experiment, each measurement was performed three times and the mean value and standard deviation were calculated 19,20 .…”

“…Inhibiting these two digestive enzymes using bioactive compounds derived from plants has been shown to be one of the most efficient strategies for managing hyperglycemia 19,30 . We investigated the α-amylase inhibitory activity of a methanolic extract of C. viscosum using acarbose as a standard α-amylase enzyme inhibitor.…”

Phytochemical Analysis and α-Amylase Inhibitory Activity of Young and Mature Leaves of Cinnamomum tamala

“…Crude extract of young and mature leaves of C. tamala was estimated for TPC by using FC reagent [30,31,32]. In brief, 20 μL sample was added with 100 μL Folinciocalteu (2 N), accompanied by 80 μL Na2CO3 (1N).…”

“…Crude extract of young and mature leaves of C. tamala was estimated for TFC by using the aluminum trichloride (AlCl3) method [32,33]. Briefly, 20 μL sample with 110 μL distilled water was added with 60 μL ethanol, 5 μL AlCl3 (10%), and 5 μL potassium acetate (1 M).…”