Phytochemical Analysis, HPLC Profiling and Anti-oxidant Potential of Euphorbia nivulia Buch.-Ham

Younus, Muhammad; Hasan, Muhammad Mohtasheemul; Ijaz, S.; Riaz, Razia; Ahmad, Khalil; Hanif, Muhammad; Khan, Mohsin Abbas; Abdullah, Muhammad Imran; Shah, Mussawar; Nisar, Uzair

doi:10.9734/jpri/2021/v33i27a31494

Cited by 1 publication

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“…Crude drugs may also be assessed qualitatively by their fluorescence pattern [42]. The fluorescence analysis of the extract showed different fluorescent colors when treated with different organic and inorganic solvents and observed under daylight and UV light.…”

Section: Discussionmentioning

confidence: 99%

Pharmacognostic Evaluation, Chemical Characterization, and Antibacterial Activity of Bassia indica (Wight) A.J. Scott

Anjum,

Touqeer,

Khan

et al. 2024

Plants

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Bassia indica (Wight) A.J. Scott is an Indian origin plant with documented medicinal and nutritional value, but has not been fully characterized yet. The present study was designed to establish pharmacognostic standards for the proper identification of the B. indica plant and its chemical characterization. The plant was standardized with World Health Organization (WHO) standardization tools and chemically characterized by Fourier transform infrared spectroscopy (FTIR) and gas chromatography-mass spectroscopy (GC-MS) analysis. Antibacterial potential was assessed by the zone of inhibition and minimum inhibitory concentration (MIC), and molecular docking studies were also performed. Pharmacognostic evaluation established the macroscopic and microscopic parameters for the identification of whole plant and its powder. Physicochemical parameters were also set forth while quantitative phytochemical analysis showed that the ethyl acetate fraction had the highest quantity of phenols, flavonoids, and tannins. FTIR analysis showed several functional groups such as phenols, alkanes, and alcohols while 55 phytochemicals were identified in the GC-MS analysis of the crude fraction. The crude extract and other fractions showed marked antibacterial activity, while the ethyl acetate fraction showed the least MIC (1.95–31.25 mg/mL). Phytochemicals identified in the GC-MS showed good molecular docking interactions against the DNA gyrase subunit B of bacteria with binding energies ranging from −4.2 to −9.4 kcal/mol. The current study describes the pharmacognostic characterization and phytochemical profiling of B. indica and provides scientific evidence to support its use in infections.

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Section: Discussionmentioning

confidence: 99%