Phytochemical and biological studies on Acanthus hirsutus Boiss

Harput-Hudaverdi, U. S.; Nagatsu, Akito; Saraçoğlu, İclal

doi:10.1055/s-0028-1084500

Cited by 2 publications

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“…and MRSA, where flavonoids and cardiac glycosides showed a broad spectrum activity, thus, justifying its candidature as a potential antimicrobial and antibiofilm drug of future. To add up on the bioactive potential of the plant, the flavonoids were screened for their cytotoxicity against three cancerous cell lines and our results are in consonance with those obtained for compounds of Acanthus hirsutus Boiss (11.17-700 µg/ml), Euphorbia hirta (625 µg/ml) [52,53] and some naturally occurring quinones [54] . These results are in consonance with other studies where plant extracts were found to be non toxic [12,[16][17][18].…”

Section: Discussionsupporting

confidence: 80%

Bioprospecting the antimicrobial, antibiofilm and antiproliferative activity of Symplocos racemosa Roxb. Bark phytoconstituents along with their biosafety evaluation and detection of antimicrobial components by GC-MS

Sood

Kumar

Gupta

et al. 2020

Preprint

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Background: The increased occurrence of the antibiotic resistance has added up to the misery of mankind. There is an immediate need to search alternatives and Symplocos racemosa is one such potent medicinal plant . The aim of the study was to explore various activities of its phytoconstituents, confirm their biosafety and identify the active components.Methods: The ethylacetate extract and the major group of phytoconstituents were tested for minimal inhibitory concentration, viable cell count studies, Post antibiotic effect, activity against 34 multi-drug resistant microorganisms, anti-biofilm and anti proliferative potential. The biosafety was evaluated by Ames and MTT assay. In vivo safety of flavonoids was determined by acute oral toxicity test using Swiss albino mice. The antimicrobial components in flavonoids were also identified by using TLC and GC-MS analysis.Results: Ethyl acetate was the best organic extractant. The phytochemical analyses revealed the presence of flavonoids, cardiac glycosides, saponins, tannins, triterpenes and Phytosterols. Flavonoids exhibited the highest antimicrobial potential whereas tannins, triterpenes and phytosterols were completely inactive. Their Minimum Inhibitory Concentration ranged from 0.5-10 mg/ml; exhibited a bactericidal mode of action and a prolonged post antibiotic effect. The phytoconstituents displayed a significant antibiofilm potential by inhibiting the initial cell attachment, disrupting the pre-formed biofilms and reducing the metabolic activity of biofilms. The phytoconstituents were significantly active against the drug-resistant strains of E.coli , MRSA and Salmonella spp. Flavonoids showed significant cytotoxic effect against the RD, L20B and Hep2 cell lines (IC 50 361-494 µg/ml). All the test extracts were biosafe as depicted by the Ames test, MTT assay. Also, flavonoids did not induce any abnormality in biochemical parameters and Organs’ histopathology of the Swiss albino mice during the in vivo acute oral toxicity studies. The flavonoids were resolve into 4 bands (S1-S4) using TLC, where S3 was the most active and its GC-MS analysis revealed the presence of Bicyclo [2.2.1]heptan-2-one,1,7,7-trimethyl-, (1S)- at an RT of 11.14 as the major compound.Conclusions: These findings suggest that Symplocos racemosa bark could be a potential source of antimicrobial metabolites, which could substantially contribute to the arsenal of existing natural drugs for combating the antimicrobial resistance.

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Section: Discussionsupporting

confidence: 80%

Bioprospecting the antimicrobial, antibiofilm and antiproliferative activity of Symplocos racemosa Roxb. Bark phytoconstituents along with their biosafety evaluation and detection of antimicrobial components by GC-MS

Sood

Kumar

Gupta

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…and MRSA, where flavonoids and cardiac glycosides showed a broad spectrum activity, thus, justifying its candidature as a potential antimicrobial and antibiofilm drug of future. To add up on the bioactive potential of the plant, the most active phytoconstituents, i.e., flavonoids were screened for their cytotoxicity against three cancerous cell lines and our results are in consonance with those obtained for compounds of Acanthus hirsutus Boiss (11.17–700 μg/mL), Euphorbia hirta (625 μg/mL) [ 53 , 54 ] and some naturally occurring quinones [ 55 ] .…”

Section: Discussionsupporting

confidence: 71%

Bioprospecting the antimicrobial, antibiofilm and antiproliferative activity of Symplocos racemosa Roxb. Bark phytoconstituents along with their biosafety evaluation and detection of antimicrobial components by GC-MS

Sood

Kumar

Gupta

et al. 2020

BMC Pharmacol Toxicol

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Background Plants provide a ray of hope to combat the ever increasing antibiotic resistance and Symplocos racemosa is a valuable medicinal plant. The study focused on highlighting the importance of this plant’s phytoconstituents as potential source of novel antimicrobials against planktonic as well as biofilm forming microorganisms, along with their antiproliferative activity. The biosafety of the phytoconstituents was also established, followed by detection of probable antimicrobial components. Methods The best organic extractant and major groups of phytoconstituents were tested for their antimicrobial activity against reference microbial strains and drug-resistant clinical isolates. The anti-proliferative potential of the most active group of phytoconstituents was evaluated against cancerous cell lines. The in vitro biosafety of phytoconstituents was evaluated by Ames and MTT assay, while in vivo biosafety of the most active phytoconstituents, i.e., flavonoids was determined by acute oral toxicity. Further, the probable antimicrobial components in the flavonoids were detected by TLC and GC-MS. Results Ethyl acetate extract was the most effective among various organic extracts, whereas phytoconstituents such as flavonoids, cardiac glycosides, saponins, tannins, triterpenes and phytosterols were the major groups present, with flavonoids being the most potent antimicrobials. The phytoconstituents displayed a significant antibiofilm potential, as exhibited by inhibition of initial cell attachment, disruption of the pre-formed biofilms and reduced metabolic activity of biofilms. The phytoconstituents were significantly active against the drug-resistant strains of E.coli, MRSA and Salmonella spp. Further, flavonoids showed significant cytotoxic effect against the cancerous cell lines but were non-cytotoxic against Vero (normal) cell line. All the test preparations were biosafe, as depicted by the Ames test and MTT assay. Also, flavonoids did not induce any abnormality in body weight, clinical signs, biochemical parameters and organs’ histopathology of the Swiss albino mice during in vivo acute oral toxicity studies. The flavonoids were resolved into 4 bands (S1-S4), where S3 was the most active and its GC-MS analysis revealed the presence of a number of compounds, where Bicyclo [2.2.1]heptan-2-one,1,7,7-trimethyl-, (1S)- was the most abundant. Conclusions These findings suggest that the phytoconstituents from Symplocos racemosa bark could act as potential source of antimicrobial as well as antiproliferative metabolites.

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Phytochemical and biological studies on Acanthus hirsutus Boiss

Cited by 2 publications

References 0 publications

Bioprospecting the antimicrobial, antibiofilm and antiproliferative activity of Symplocos racemosa Roxb. Bark phytoconstituents along with their biosafety evaluation and detection of antimicrobial components by GC-MS

Bioprospecting the antimicrobial, antibiofilm and antiproliferative activity of Symplocos racemosa Roxb. Bark phytoconstituents along with their biosafety evaluation and detection of antimicrobial components by GC-MS

Bioprospecting the antimicrobial, antibiofilm and antiproliferative activity of Symplocos racemosa Roxb. Bark phytoconstituents along with their biosafety evaluation and detection of antimicrobial components by GC-MS

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