Phytochemical and enzyme inhibitory studies on indigenous medicinal plant<i>Rhazya stricta</i>

Sultana, Nighat; Khalid, Asaad

doi:10.1080/14786410802417040

Cited by 29 publications

(24 citation statements)

References 16 publications

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“…Furthermore, the crude ethanolic extract of R. stricta fruit has shown anti-bacterial and lipoxygenase activities, as well as acetylcholinesterase inhibitory effect (Sultana and Khalid 2010). It was reported that the crude extract of R. stricta potently inhibited cellular growth and colony formation of human breast and lung cancer cell clones through apoptosis induction (Baeshen et al 2012;Elkady 2013).…”

Section: Introductionmentioning

confidence: 97%

Establishment of transgenic Rhazya stricta hairy roots to modulate terpenoid indole alkaloid production

et al. 2015

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Transgenic hairy roots of R. stricta were developed for investigation of alkaloid accumulations. The contents of five identified alkaloids, including serpentine as a new compound, increased compared to non-transformed roots. Rhazya stricta Decne. is a rich source of pharmacologically active terpenoid indole alkaloids (TIAs). In order to study TIA production and enable metabolic engineering, we established hairy root cultures of R. stricta by co-cultivating cotyledon, hypocotyl, leaf, and shoot explants with wild-type Agrobacterium rhizogenes strain LBA 9402 and A. rhizogenes carrying the pK2WG7-gusA binary vector. Hairy roots initiated from the leaf explants 2 to 8 weeks. Transformation was confirmed by polymerase chain reaction and in case of GUS clones with GUS staining assay. Transformation efficiency was 74 and 83% for wild-type and GUS hairy root clones, respectively. Alkaloid accumulation was monitored by HPLC, and identification was achieved by UPLC-MS analysis. The influence of light (16 h photoperiod versus total darkness) and media composition (modified Gamborg B5 medium versus Woody Plant Medium) on the production of TIAs were investigated. Compared to non-transformed roots, wild-type hairy roots accumulated significantly higher amounts of five alkaloids. GUS hairy roots contained higher amounts two of alkaloids compared to non-transformed roots. Light conditions had a marked effect on the accumulation of five alkaloids whereas the composition of media only affected the accumulation of two alkaloids. By successfully establishing R. stricta hairy root clones, the potential of transgenic hairy root systems in modulating TIA production was confirmed.

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Section: Introductionmentioning

confidence: 97%

Establishment of transgenic Rhazya stricta hairy roots to modulate terpenoid indole alkaloid production

et al. 2015

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“…Phytochemical studies conducted on various parts of R . stricta have revealed the presence of over a hundred monomeric and dimeric TIAs and few non‐alkaloid components (Ali et al ., ; Gilani et al ., ; Sultana and Khalid, ). The alkaloids, classified into 17 sub‐groups (Atta‐ur‐Rahman et al ., ), have been widely reported since the early 1960s, and most of them have been isolated from the leaves (Ali et al ., ).…”

Section: Introductionmentioning

confidence: 99%

“…Plants in the family are well known for the presence of an exclusive array of terpenoid indole alkaloids (TIAs) with a diverse range of biological activities. Phytochemical studies conducted on various parts of R. stricta have revealed the presence of over a hundred monomeric and dimeric TIAs and few non-alkaloid components (Ali et al, 2000;Gilani et al, 2007;Sultana and Khalid, 2010). The alkaloids, classified into 17 sub-groups (Atta-ur-Rahman et al, 1989), have been widely reported since the early 1960s, and most of them have been isolated from the leaves (Ali et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Determination of terpenoid indole alkaloids in hairy roots of Rhazya stricta (Apocynaceae) by GC‐MS

Akhgari

Laakso

Seppänen‐Laakso

et al. 2015

Phytochemical Analysis

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“…Authors also reported that the phytosterol mixture was a better AchE inhibitor than its pure compound on C. quinquefasciatus AchE [23]. Other authors reported non-activity of stigmasterol in a mice model and in an vitro test at a dose of 0.3-30 µM (0.123 mg/L, 12.3 mg/L) [24], while other authors reported AchE inhibition with IC 50 of 644 µM (265 mg/L) [25].…”

Section: In Vitro Bioassaysmentioning

confidence: 98%

Himalayan Nettle Girardinia diversifolia as a Candidate Ingredient for Pharmaceutical and Nutraceutical Applications—Phytochemical Analysis and In Vitro Bioassays

et al. 2020

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Girardinia diversifolia, also known as Himalayan nettle, is a perennial herb used in Nepal to make fiber as well as in traditional medicine for the treatment of several diseases. To date, phytochemical studies and biological assays on this plant are scarce. Thus, in the present work, the G. diversifolia extracts have been evaluated for their potential pharmaceutical, cosmetic and nutraceutical uses. For this purpose, detailed phytochemical analyses were performed, evidencing the presence of phytosterols, fatty acids, carotenoids, polyphenols and saponins. The most abundant secondary metabolites were β- and γ-sitosterol (11 and 9% dw, respectively), and trans syringin (0.5 mg/g) was the most abundant phenolic. Fatty acids with an abundant portion of unsaturated derivatives (linoleic and linolenic acid at 22.0 and 9.7 mg/g respectively), vitamin C (2.9 mg/g) and vitamin B2 (0.12 mg/g) were also present. The antioxidant activity was moderate while a significant ability to inhibit acetylcholinesterase (AChE), butyrilcholinesterase (BuChE), tyrosinase, α-amylase and α-glucosidase was observed. A cytotoxic effect was observed on human ovarian, pancreatic and hepatic cancer cell lines. The effect in hepatocarcinoma cells was associated to a downregulation of the low-density lipoprotein receptor (LDLR), a pivotal regulator of cellular cholesterol homeostasis. These data show the potential usefulness of this species for possible applications in pharmaceuticals, nutraceuticals and cosmetics.

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Phytochemical and enzyme inhibitory studies on indigenous medicinal plantRhazya stricta

Cited by 29 publications

References 16 publications

Establishment of transgenic Rhazya stricta hairy roots to modulate terpenoid indole alkaloid production

Establishment of transgenic Rhazya stricta hairy roots to modulate terpenoid indole alkaloid production

Determination of terpenoid indole alkaloids in hairy roots of Rhazya stricta (Apocynaceae) by GC‐MS

Himalayan Nettle Girardinia diversifolia as a Candidate Ingredient for Pharmaceutical and Nutraceutical Applications—Phytochemical Analysis and In Vitro Bioassays

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