Phytochemical and in vitro biological investigations of methanolic extracts of Enhydra fluctuans Lour.

Kuri, Sourov; Billah, Md. Mustahsan; Rana, S. M. Masud; Naim, Zannatul; Islam, Md. Mahmodul; Hasanuzzaman, Md.; Ali, Md. Ramjan; Banik, Rana

doi:10.12980/apjtb.4.2014c677

Cited by 27 publications

(14 citation statements)

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“…I. aquatica has been used in folk medicine against different diseases including diabetes [ 11 ], hypertension [ 12 ], liver malfunction [ 13 ], constipation [ 14 ] and in the treatment of opium and arsenic poisoning [ 15 ]. On other hand, E. fluctuans possesses ethnomedicinal significance as anti-inflammatory [ 16 ], anthelmintic [ 16 ], analgesic [ 17 ], hepatoprotective [ 18 ] and hypotensive [ 18 ] agent. A Literature survey revealed presence of flavonoids, phenolic compounds, β-carotene and ascorbic acid in both the plants [ 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

“…On other hand, E. fluctuans possesses ethnomedicinal significance as anti-inflammatory [ 16 ], anthelmintic [ 16 ], analgesic [ 17 ], hepatoprotective [ 18 ] and hypotensive [ 18 ] agent. A Literature survey revealed presence of flavonoids, phenolic compounds, β-carotene and ascorbic acid in both the plants [ 18 , 19 ]. Considering the presence of phyto-antioxidant molecules, the present study was designed to evaluate the possible protective role of aqueous extracts of the two aforementioned edible plants in Cd-toxicity.…”

Section: Introductionmentioning

confidence: 99%

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The effects of two common edible herbs, Ipomoea aquatica and Enhydra fluctuans, on cadmium-induced pathophysiology: a focus on oxidative defence and anti-apoptotic mechanism

et al. 2015

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BackgroundIpomoea aquatica (Convolvulaceae) and Enhydra fluctuans (Asteraceae), two aquatic vegetables, are traditionally used against heavy metal toxicity in traditional medicines in India. The present study aimed to explore the protective role of edible (aqueous) extracts of I. aquatica (AEIA) and E. fluctuans (AEEF) against Cd-intoxication.MethodsThe extracts were chemically standardized by spectroscopic and HPLC analysis. The cytoprotective roles of AEIA and AEEF were measured on mouse hepatocytes. The effect on redox status were measured after incubating the hepatocytes with CdCl2 (30 μM) along with AEIA or AEEF (400 μg/ml). The effects on the expressions of apoptotic signal proteins were estimated. The protective roles of AEIA or AEEF were measured by in vivo assay in mice. Haematological, serum biochemical, tissue redox status, Cd bioaccumulation and histological parameters were evaluated to estimate the protective role of AEIA or AEEF (100 mg/kg) against CdCl2 (4 mg/kg) intoxication.ResultsPhytochemical analysis revealed presence of substantial quantities of phenolics, flavonoids, saponins, carbohydrates and ascorbic acid in AEIA or AEEF. CdCl2 treated murine hepatocytes showed a gradual reduction of cell viability in a concentration dependent manner with an IC50 of ~30 μM. CdCl2 treated hepatocytes exhibited significantly enhanced levels (p < 0.01) of ROS production, lipid peroxidation, protein carbonylation and NADPH oxidase with concomitant depletion (p < 0.01) of antioxidant enzymes and GSH. However, AEIA or AEEF treatment along with CdCl2 significantly restored the aforementioned parameters in murine hepatocytes near to normalcy. Besides, AEIA or AEEF significantly counteracted (p < 0.05–0.01) with ROS mediated alteration of transcription levels of signal proteins viz. Bcl-2, BAD, Cyt-C, Caspases, Fas and Bid. In in vivo bioassay, CdCl2 treatment caused significantly high Cd bioaccumulation and oxidative stress in the liver, kidney, heart, brain and testes in mice. In addition, the haematological and serum biochemical parameters were significantly altered in the CdCl2 treated animals. Simultaneous administration of AEIA or AEEF could significantly restore the tested parameters to the near-normal status.ConclusionThe extracts would offer the overall protective effect via counteracting with Cd mediated oxidative stress and/or promoting the elimination of Cd by chelating.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The effects of two common edible herbs, Ipomoea aquatica and Enhydra fluctuans, on cadmium-induced pathophysiology: a focus on oxidative defence and anti-apoptotic mechanism

et al. 2015

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“…Test for alkaloids: To 0.5 g of each (petroleum ether, chloroform and chloroform / methanol 1:1) crude extracts were added to 1 mL of Mayer's reagent and few drop of iodine solution. Formation of yellow colour precipitate indicates the presence of alkaloids [31].…”

Section: Phytochemical Screeningmentioning

confidence: 99%

Phytochemical investigation and evaluation of antimicrobial activities of stem bark of Morella salicifolia

Meniso¹,

Boru²,

Ganjinaboyina³

2019

Bull. Chem. Soc. Eth.

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The Morella salicifolia bark is used as a traditional medicine for the treatment of toothache, headache and stomachache in the eastern Ethiopia. The stem bark of the plant was sequentially extracted with: petroleum ether, chloroform and chloroform/methanol (1:1) using Soxhlet apparatus. Phytochemical screenings tests were conducted on of stem bark this plant extracts were performed on petroleum ether, chloroform and chloroform/methanol (1:1) crude extracts. The results showed presence of alkaloid, tannins, saponins, flavonoid, protein, and triterpenoids in the petroleum ether extract; alkaloid, tannin, flavonoid, protein, triterpenoid and absence of saponins chloroform extract; tannin, flavonoid, triterpenoid and absence of alkaloid, tannin, saponins and protein in the chloroform/methanol (1:1) extract. Volatile oil of the stem bark of plant was analyzed by GC-MS and it constitute 14 compounds among which the major compounds were hexadecanoic acid methyl ester (29.40%), (Z)-9-octadecenoic acids and methyl ester (28.64%). The volatile oil and crude extracts of the stem bark were tested in vitro for their antimicrobial activity by disk diffusion method. The highest activity was exhibited by volatile oil, CHCl3/MeOH (1:1) and petroleum ether extract against Shigela flexneri, Escherichia coli and Streptococcus agalactiae with an inhibition diameter of 21.66, 17.66 and 18.00 mm, respectively.

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“…There are reports on antioxidant property of the methanolic extract, antimicrobial property of the toluene, chloroform and methanolic extract of E. fluctuans, moreover CNS depressant activity, anti-inflammatory activity of E. fluctuans have also been reported [27][28][29][30]. But there are insufficient records in literature, regarding pharmacological activities and phytochemical characteristics of E. fluctuans Lour available in Bangladesh which are known to differ with plant's geographical location.…”

Section: Introductionmentioning

confidence: 99%

Phytochemical and Pharmacological Potential of Enhydra fluctuans Available in Bangladesh

Kamal

Rony

Sharmin

et al. 2019

JPRI

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Objectives: The possible phytochemical constituents, thrombolytic and membrane stabilizing activities of the crude ethanolic extract of Enhydra fluctuans (CE) were investigated along with the antimicrobial, antioxidant and cytotoxic potentials of its petroleum ether (PESF), carbon tetrachloride (CTCSF), chloroform (CSF) and aqueous (AQSF) soluble fractions. Materials & Methods: The coarse leaf powder was extracted at room temperature with ethanol. Solvent-solvent partitioning was done to obtain the four soluble fractions. Anticoagulant potential was determined by the in vitro thrombolytic model, membrane stabilization method was used to assess in vitro anti-inflammatory activity, the disc diffusion method was used for anti-microbial screening, antioxidant potential was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method and brine shrimp lethality bioassay method was used for cytotoxic activity determination. Results: Chemical screening of the crude extract evidenced the presence of alkaloids, saponins, tannins, flavonoids, reducing sugars and gums. It showed significant clot lysis property of 46.91%. It also significantly inhibited heat and hypotonic solution induced lysis of the human red blood cell membrane with values of 71.80% and 47.60%, respectively. CTCSF and PESF showed mild antimicrobial activity. AQSF showed most prominent antioxidant activity with IC50 value of 12.27 µg/mL. CTCSF showed LC50 value of 0.84 µg/mL, with most potent cytotoxic activity. Conclusion: Significant thrombolytic, membrane stabilizing, antioxidant and in vitro cytotoxic activities of the ethanolic plant extract were observed in this study. In vivo activities and isolation of active compound(s) from this extract are yet to be investigated.

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Phytochemical and in vitro biological investigations of methanolic extracts of Enhydra fluctuans Lour.

Abstract: The study proves that the crude methanolic extract of Enhydra fluctuans Lour. has significant antioxidant, anthelmintic and thrombolytic activity containing wide range of phytochemicals.

Cited by 27 publications

References 22 publications

The effects of two common edible herbs, Ipomoea aquatica and Enhydra fluctuans, on cadmium-induced pathophysiology: a focus on oxidative defence and anti-apoptotic mechanism

The effects of two common edible herbs, Ipomoea aquatica and Enhydra fluctuans, on cadmium-induced pathophysiology: a focus on oxidative defence and anti-apoptotic mechanism

Phytochemical investigation and evaluation of antimicrobial activities of stem bark of Morella salicifolia

Phytochemical and Pharmacological Potential of Enhydra fluctuans Available in Bangladesh

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