Phytochemical and Morphological Evidences for Shikonin Production by Plant Cell Cultures of Onosma sericeum Willd

Gharehmatrossian, Sormeh; Popov, Yu. G.; Ghorbanli, M; Safaeian, Shila; Iranbakhsh, Alıreza

doi:10.1590/1678-4324-2016160235

Cited by 7 publications

(4 citation statements)

References 26 publications

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“…A. DC. [ 12 ], Onosma bulbotrichum [ 13 ], and Onosma sericeum Willd [ 14 ]. In this study, the highest callus proliferation was attained after 5 weeks of cultivation under a 16-h photoperiod on MS medium augmented with 4 µM of each 6-BA and NAA.…”

Section: Discussionmentioning

confidence: 99%

Screening of Bioactive Metabolites and Biological Activities of Calli, Shoots, and Seedlings of Mertensia maritima (L.) Gray

Song

Sivanesan

et al. 2020

Plants

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Mertensia maritima (L.) Gray is threatened with extinction owing to climate change, poor seed germination, and ocean warming. In vitro explant-culture is used for ex situ preservation and plantlet massive production. In vitro cell and organ cultures serve as an alternative plant material source to investigate the biological activities and phytochemical profiles of rare plants. We aimed to develop an efficient callus and shoot production protocol and investigate bioactive metabolites, antioxidants, and enzyme inhibitory potential of M. maritima calli, shoots, and in vivo seedlings. The effects of combinations of different plant growth regulators, 6-BA (N6-benzyladenine), 6-KN (Kinetin), TDZ (Thidiazuron), and NAA (1-Naphthylacetic acid), in MS (Murashige and Skoog) nutrient medium were studied. The highest callus proliferation was obtained after 5-week cultivation over a 16-h photoperiod on growth medium MS enriched with 4 µM each of 6-BA and NAA. The medium with 2 µM 6-BA and 4 µM 6-KN had the best shoot induction rate (91.1%) with a mean of 13.4 shoots. The combination of two cytokinins (6-BA and 6-KN) was found to be effective in M. maritima shoot regeneration. The rooting frequency was 100% in ½ MS with Indole-3-butyric acid (IBA 2 µM). The number of detected compounds and chemical composition in the M. maritima shoots and seedlings extracts were similar. The total amount of phenolics in the shoots was 216.4% and 369.5% higher than in seedlings and calli, respectively. The total amount of flavonoids in the shoots was 241.1% and 429.3% higher than in seedlings and calli, respectively. The best antioxidant activity was obtained in the shoots, followed by seedlings and calli. However, the order was seedlings > calli > shoots regarding metal chelating ability. The strongest acetylcholinesterase inhibition properties were obtained in the calli, followed by seedlings and shoots. However, the tested samples can be ranked as seedlings > shoots > calli in butylcholinestrase inhibition assay. This study is the first report on the enzyme inhibitory effects of M. maritima extracts, providing valuable contributions to the scientific community.

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Section: Discussionmentioning

confidence: 99%

Screening of Bioactive Metabolites and Biological Activities of Calli, Shoots, and Seedlings of Mertensia maritima (L.) Gray

Song

Sivanesan

et al. 2020

Plants

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“…Although there are many reports about secondary metabolites production by plant cell culture, only a few of them have been produced in large-scale, such as paclitaxel ( Li et al, 2009 ), ginseng ( Hu et al, 2001 ) and shikonin ( Sormeh et al, 2016 ) etc. Other species, however, cannot be utilized on a large scale probably due to the low contents and yields of active compounds.…”

Section: Introductionmentioning

confidence: 99%

Investigating the induction of polyphenol biosynthesis in the cultured Cycolocarya paliurus cells and the stimulatory mechanism of co-induction with 5-aminolevulinic acid and salicylic acid

Ling

Wang²,

Pan³

et al. 2023

Front. Bioeng. Biotechnol.

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Background: Plant cell culture technology is a potential way to produce polyphenols, however, this way is still trapped in the dilemma of low content and yield. Elicitation is regarded as one of the most effective ways to improve the output of the secondary metabolites, and therefore has attracted extensive attention.Methods: Five elicitors including 5-aminolevulinic acid (5-ALA), salicylic acid (SA), methyl jasmonate (MeJA), sodium nitroprusside (SNP) and Rhizopus Oryzae Elicitor (ROE) were used to improve the content and yield of polyphenols in the cultured Cyclocarya paliurus (C. paliurus) cells, and a co-induction technology of 5-ALA and SA was developed as a result. Meanwhile, the integrated analysis of transcriptome and metabolome was adopted to interpret the stimulation mechanism of co-induction with 5-ALA and SA.Results: Under the co-induction of 50 μM 5-ALA and SA, the content and yield of total polyphenols of the cultured cells reached 8.0 mg/g and 147.12 mg/L, respectively. The yields of cyanidin-3-O-galactoside, procyanidin B1 and catechin reached 28.83, 4.33 and 2.88 times that of the control group, respectively. It was found that expressions of TFs such as CpERF105, CpMYB10 and CpWRKY28 increased significantly, while CpMYB44 and CpTGA2 decreased. These great changes might further make the expression of CpF3′H (flavonoid 3′-monooxygenase), CpFLS (flavonol synthase), CpLAR (leucoanthocyanidin reductase), CpANS (anthocyanidin synthase) and Cp4CL (4-coumarate coenzyme A ligase) increase while CpANR (anthocyanidin reductase) and CpF3′5′H (flavonoid 3′, 5′-hydroxylase) reduce, ultimately enhancing the polyphenols accumulationConclusion: The co-induction of 5-ALA and SA can significantly promote polyphenol biosynthesis in the cultured C. paliurus cells by regulating the expression of key transcription factors and structural genes associated with polyphenol synthesis, and thus has a promising application.

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“…It is well known that some species under the family Boraginaceae (Onosma Sericeum Willd., Onosma Microcarpum Steven ex D.C.) produce wound healing effects in traditional application, and are used for the treatment of burns (10). Onosma Sericeum Willd in particular is used in diverse areas such as food and textile thanks to the red pigments in its roots (11). Onosma sericeum, the subject of this study, has been used to treat injuries and burns in Adıyaman for many years.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Changes Biological Activity of Onosma Sericeum Willd (Boraginaceae) Based on Collection Time and Extraction Solvent, and Determination of Its Mineral and Trace Element Composition

Doğan

Gündoğan

2019

Journal of the Turkish Chemical Society Section A: Chemistry

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In this study, total phenol, flavonoid, and free radical scavenging activity of Onosma Sericeum Willd (Boraginaceae), a plant grown in Turkey and traditionally used for wound healing, were determined by using spectrophotometric methods. Its cytotoxic effect on breast cancer cells (MCF-7) was investigated. Furthermore, mineral and trace element composition of the plant roots, collected in different times, was determined by using inductively coupled plasma mass spectroscopy (ICP-MS). Onosma Sericeum Willd, which was collected in Besni region, Adıyaman in May, June and July and dried properly, was extracted by using different solvents in the study. Total phenol content of the extracts was calculated by using the Folin-Ciocalteu method in gallic acid equivalent as 58.42±0.13-93.34±0.68 mg (GAE)/g. The flavonoid content was calculated as 21.79±1.3-60.33±0.36 mg (KUE)/g in quercetin equivalent by using the aluminum chloride method. Free radical scavenging activity was measured spectrophotometrically by using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. DPPH radical scavenging activity rates were found to be in the range of 60.27±0.06-78.60±0.21% in the form of % inhibition. It was observed that extracts which were prepared by using ethanol produced the cytotoxic effect on the MCF-7 cancer cells depending on the dosage. It was concluded that the rates of phenol, flavonoid, free radical scavenging activity, and cytotoxic effect differed based on the solvent used for extraction and the time of plant collection. Moreover, trace element and mineral composition of the plant samples, collected in different times, were determined.

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Phytochemical and Morphological Evidences for Shikonin Production by Plant Cell Cultures of Onosma sericeum Willd

Abstract: ABSTRACT

Cited by 7 publications

References 26 publications

Screening of Bioactive Metabolites and Biological Activities of Calli, Shoots, and Seedlings of Mertensia maritima (L.) Gray

Screening of Bioactive Metabolites and Biological Activities of Calli, Shoots, and Seedlings of Mertensia maritima (L.) Gray

Investigating the induction of polyphenol biosynthesis in the cultured Cycolocarya paliurus cells and the stimulatory mechanism of co-induction with 5-aminolevulinic acid and salicylic acid

Evaluation of Changes Biological Activity of Onosma Sericeum Willd (Boraginaceae) Based on Collection Time and Extraction Solvent, and Determination of Its Mineral and Trace Element Composition

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