Phytochemical Characterisation of Green Beans (<i>Phaseolus vulgaris L</i>.) by Using High‐performance Liquid Chromatography Coupled with Time‐of‐flight Mass Spectrometry

Abu‐Reidah, Ibrahim M.; Arráez‐Román, David; Lozano‐Sánchez, Jesús; Segura‐Carretero, Antonio; Fernández‐Gutiérrez, Alberto

doi:10.1002/pca.2385

Cited by 72 publications

(49 citation statements)

References 40 publications

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“…There are numerous reports on the phytochemical content of beans. Phenolic profiles have been well investigated in many dry bean samples (Luthria and Pastor‐Corrales ; Lin and others ; Abu‐Reidah and others ; Limón and others ). Effect of growing conditions on yield of dry beans has been reviewed in earlier publications by Jolley and others () and Fageria and Santos ().…”

Section: Introductionmentioning

confidence: 99%

Amino Acid, Organic Acid, and Sugar Profiles of 3 Dry Bean (Phaseolus vulgaris L.) Varieties

John

Luthria

2015

Journal of Food Science

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In this study, we compared the amino acid, organic acid and sugar profiles of 3 different varieties of dry beans (black bean [BB], dark red bean [DRB], and cranberry bean [CB]). The efficiency of the 2 commonly used extraction solvents (water and methanol:chloroform:water [2.5:1:1, v/v/v/]) for cultivar differentiation based on their metabolic profile was also investigated. The results showed that the BB contained the highest concentration of amino acids followed by DRB and CB samples. Phenylalanine, a precursor for the biosynthesis of phenolic secondary metabolites was detected at low concentration in CB samples and correlated with the reduced anthocyanins content in CB extract as documented in the published literature. Comparing the extractability of 2 extraction solvents, methanol:chloroform:water (2.5:1:1, v/v/v/) showed higher recoveries of amino acids from 3 beans, whereas, sugars were extracted in higher concentration with water. Analytically, gas chromatography detected sugars (9), amino acids (11), and organic acids (3) in a single run after derivatization of the extracts. In comparison, ion chromatography detected only sugars in a single run without any derivatization step with the tested procedure. Bean samples are better differentiated by the sugar content extracted with water as compared to the aqueous organic solvent extracts using partial least-square discriminant analysis.

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Section: Introductionmentioning

confidence: 99%

Amino Acid, Organic Acid, and Sugar Profiles of 3 Dry Bean (Phaseolus vulgaris L.) Varieties

John

Luthria

2015

Journal of Food Science

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“…11 Although some completely separated or high-concentration compounds in complex matrix have been identied by TOF/MS they depend on the accurate mass weight of the quasi-molecular ions and fragment ions obtained by dynamic adjustment of the fragmentor voltage, 12 for trace or co-eluting constituents, the recognition of their fragment ions has been proved to be a problem. 11 Although some completely separated or high-concentration compounds in complex matrix have been identied by TOF/MS they depend on the accurate mass weight of the quasi-molecular ions and fragment ions obtained by dynamic adjustment of the fragmentor voltage, 12 for trace or co-eluting constituents, the recognition of their fragment ions has been proved to be a problem.…”

Section: Introductionmentioning

confidence: 99%

An effective integrated method for comprehensive identification of eighty-five compounds in Zhi-Zi-Da-Huang decoction by HPLC-DAD-ESI-MS (TOF) and HPLC-DAD-ESI-MS/MS (QqQ) without the help of reference standards

Ling-ling

Shi

et al. 2014

Anal. Methods

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decoction (ZZDHD) is an effective formula to treat alcoholic liver disease in China for nearly 2000 years. However, compounds in the formula are still not clear due to the complexity of the coexisting constituents. In this study, a sensitive integrated method based on HPLC-DAD-ESI-MS (TOF) and HPLC-DAD-ESI-MS/MS (QqQ) was developed and 85 chemical compounds in ZZDHD were identified. By TOF/MS, the quasi-molecular ions of the constituents were deduced according to the accurate mass of corresponding positive and negative ions, and product ion scan was then carried out using QqQ/MS. The fragments of each compound were observed by TOF/MS and/or QqQ/MS. Compounds with different chemical structures and concentration levels were tentatively identified according to their chromatographic retention time, UV absorption and MS data. Fragmentation patterns of six types were proposed to verify the identification results. It was found that at TOF/MS mode, a retro diels-alder (RDA) reaction occurred at bonds 1 and 3 in C-ring of flavanone 7-O-diglycosides when 4 0 -OH exists at B-ring. While the reaction was not observed if a methoxyl group appeared at the same site of flavanone skeleton. Another important phenomenon was noticed that 3-sinapoyl-5-caffeoylquinic acid, 4-sinapoyl-3-caffeoylquinic acid, and sinapylglucoside were detected only in ZZDHD while they were not found in four component herb extracts. The phenomenon revealed that changes of compounds happened when the component herbs were decocted together. The results in the paper are favorable of the clinic dosage and have laid a foundation for the subsequent research of ZZDHD in vivo.

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“…The snap bean (Phaseolus vulgaris L.) is a vegetable characterized by fresh pods that are relatively rich in vitamins, minerals, and antioxidants (ABU-REIDAH et al, 2013). In Brazil, the production data of this vegetable are incipient.…”

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confidence: 99%

Production components and yield of bushing snap bean in conventional and organic production systems

Gomes

Furlan

Freiria

et al. 2017

SCA

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Production systems influence crops differently, mainly in terms of yield. However, there are few studies that have evaluated different bushing snap bean genotypes in different systems. Thus, this study aimed to evaluate the production components and yield of bushing snap beans in conventional and organic production systems. The experimental design was a randomized complete block, in a factorial 6 × 2 arrangement, corresponding to six genotypes and two production systems, with three replications. The genotypes Isla Manteiga Baixo ®

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Phytochemical Characterisation of Green Beans (Phaseolus vulgaris L.) by Using High‐performance Liquid Chromatography Coupled with Time‐of‐flight Mass Spectrometry

Cited by 72 publications

References 40 publications

Amino Acid, Organic Acid, and Sugar Profiles of 3 Dry Bean (Phaseolus vulgaris L.) Varieties

Amino Acid, Organic Acid, and Sugar Profiles of 3 Dry Bean (Phaseolus vulgaris L.) Varieties

An effective integrated method for comprehensive identification of eighty-five compounds in Zhi-Zi-Da-Huang decoction by HPLC-DAD-ESI-MS (TOF) and HPLC-DAD-ESI-MS/MS (QqQ) without the help of reference standards

Production components and yield of bushing snap bean in conventional and organic production systems

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