Phytochemical characterization and in vitro antibacterial activity of Xeroderris stuhlmannii (Taub.) Mendonca &amp; E.P. Sousa bark extracts

Selemani, Major A.; Kazingizi, Luckmore F.; Manzombe, Emily; Bishi, Lorraine Y.; Mureya, Cleopas; Gwata, Tichaziwa T.; Rwere, Freeborn

doi:10.1016/j.sajb.2021.07.006

Cited by 9 publications

(14 citation statements)

References 33 publications

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“…The reported IC50 values for acarbose are closer to those reported in literature [37]. The high potency of the extracts (low IC50 values) against digestive enzymes are likely due to the presence of a number of bioactive phytochemical compounds acting synergistically to inhibit the enzymes [16].…”

Section: Discussionsupporting

confidence: 78%

“…The copyright holder for this preprint this version posted September 19, 2022. ; https://doi.org/10.1101/2022.09. 16.508336 doi: bioRxiv preprint phytochemical compounds reduce glucose absorption in the gastrointestinal tract by inhibiting digestive enzymes (such as α-glucosidases) [14]. High blood glucose is associated with many comorbidities such as heart disease, stroke, kidney disease, eye problems, dental disease, nerve damage and feet problems that reduce the quality of life and life expectancy of diabetic patients [15].…”

Section: Introductionmentioning

confidence: 99%

“…Mendonca & E.P. Sousa by Gas chromatography mass spectroscopy (GC MS) and liquid chromatography tandem mass spectroscopy (LC MS/MS) studies showed the presence of thirtysix (36) important bioactive phytochemical compounds categorized into phenolic, flavonoid and alkaloid compounds [16]. Whether some of the phytochemical compounds in Xeroderris stuhlmannii (Taub.)…”

Section: Introductionmentioning

confidence: 99%

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Inhibitory andin silicomolecular docking ofXeroderris stuhlmannii(Taub.) Mendonca & E.P. Sousa phytochemical compounds on human α-glucosidases

Nyathi

Bvunzawabaya

Manzombe

et al. 2022

Preprint

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Millions of people in developing nations rely on herbal traditional medicine for the treatment of ailments such as diabetes mellitus, stomach disorders and respiratory diseases. Xeroderris stuhlmannii (Taub.) Mendonca & E.P. Sousa is a medicinal plant used traditionally in Zimbabwe to treat diabetes mellitus and its complications. However, there is no scientific evidence to support its role as an antidiabetic medicinal plant. Here we hypothesized that Xeroderris stuhlmannii (Taub.) Mendonca & E.P. Sousa contain bioactive phytochemicals that can scavenge free radicals, and inhibit digestive enzymes that contribute to type 2 diabetes mellitus (T2DM). To test this hypothesis, we examined the free radical scavenging potential of crude extracts using the diphenyl-2-picrylhydrazyl assay in vitro. Furthermore, we carried out in vitro antidiabetic activity of crude extracts using chromogenic 3,5-dinitrosalicylic acid and p-nitrophenyl-alpha-D-glucopyranoside substrates on alpha-amylase and alpha-glucosidase. In addition, we used molecular docking approaches to screen for bioactive phytochemical compounds targeting the digestive enzymes. Our results showed that phytochemicals in Xeroderris stuhlmannii (Taub.) Mendonca & E.P. Sousa extracts scavenged free radicals with IC50 values ranging from 0.011-0.013 micrograms/mL. Further, the crude extracts significantly inhibited alpha-amylase and alpha-glucosidase with IC50 values of 12.9-21.1 micrograms/mL and 8.8-16.0 micrograms/mL, respectively. In silico molecular docking findings and pharmacokinetic predictions showed that myricetin is a novel inhibitor of the digestive enzymes that contributes to high blood glucose. Collectively, our findings suggest pharmacological targeting of digestive enzymes by Xeroderris stuhlmannii (Taub). Mendonca & E.P. Sousa crude extracts could lesion type 2 mellitus complications in humans.

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Section: Discussionsupporting

confidence: 78%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Inhibitory andin silicomolecular docking ofXeroderris stuhlmannii(Taub.) Mendonca & E.P. Sousa phytochemical compounds on human α-glucosidases

Nyathi

Bvunzawabaya

Manzombe

et al. 2022

Preprint

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“…Mendonça & E.P. Sousa that are used frequently to control or treat diabetes mellitus ( Selemani et al, 2021 ). In view of this, it is important for governments to provide funds for research that promotes the use of medicinal herbs to combat many emerging pathogens and diabetes mellitus complications.…”

Section: Future Directionsmentioning

confidence: 99%

Natural products for the treatment and management of diabetes mellitus in Zimbabwe-a review

2022

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Use of medicinal plants and herbs in the treatment and management of diseases, including diabetes mellitus and its complications remains an integral part of African tradition. In Zimbabwe, nearly one million people are living with diabetes mellitus. The prevalence of diabetes mellitus in Zimbabwe is increasing every year due to lifestyle changes, and has accelerated the use of traditional medicines for its treatment and management in urban areas. In addition, the high cost of modern medicine has led many people in rural parts of Zimbabwe to rely on herbal plant medicine for the treatment of diabetes mellitus and its complications. This review highlights a number of studies carried out to evaluate the antidiabetic properties of indigenous plants found in Zimbabwe with the goal of treating diabetes mellitus. Further, we discuss the mechanism of action of various plant extracts in the treatment and management of diabetes mellitus. Together, this review article can open pathways leading to discovery of new plant derived medicines and regularization of use of crude plant remedies to treat diabetes mellitus by the Zimbabwean government and others across Africa.

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“…Xeroderris genus belongs to the Fabaceae family, commonly known as Leguminoseae , and is an extremely rich source of biologically active compounds mainly flavonoid derivatives, which is the major class of secondary metabolites found in the family [ 1 , 2 ]. These bioactive phytochemicals possess antibacterial, antioxidant, antifungal, and antimalarial activities [ 3 , 4 , 5 ]. Despite the existing various solutions for health care, drug resistance is common.…”

Section: Introductionmentioning

confidence: 99%

Rotenoids and Isoflavones from Xeroderris stuhlmannii (Taub.) Mendonça & E.P. Souza and Their Biological Activities

et al. 2023

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The phytochemical study of the ethanolic extract of the leaf of Xeroderris stuhlmannii led to the isolation of five hitherto unreported compounds including two isoflavones (1–2), and three rotenoids (3–5), along with eight known isoflavonoid derivatives (6–13) and one pterocarpan derivative (14). The structures of the new compounds and those of the known ones were established by the spectroscopic (1D and 2D NMR) and spectrometric (HRESIMS) techniques as well as a comparison of their spectroscopic data with those reported in the literature. The leaf extract, fractions, and isolated compounds were tested for their antibacterial effects against nine bacterial strains. Compounds 3, 8, 11, and 12 showed a significant antibacterial effect, with a minimum inhibitory concentration (MIC) value of 62.5 µg/mL each, against Salmonella typhi, Staphylococcus aureus, Klessiella pneumonae, and Escherichia coli, respectively. In addition, the leaf extract, fractions, and isolated compounds were tested for their antifungal effects against four fungal strains. The hexane fraction showed a significant antifungal effect with an MIC value of 125 µg/mL against Candida parasilosis, whereas compounds 3, 8, and 12 showed significant antifungal activity with an MIC value of 62.5 µg/mL, each against Candida parasilosis, Candida albicans, and Candida krusei, respectively.

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Phytochemical characterization and in vitro antibacterial activity of Xeroderris stuhlmannii (Taub.) Mendonca & E.P. Sousa bark extracts

Cited by 9 publications

References 33 publications

Inhibitory andin silicomolecular docking ofXeroderris stuhlmannii(Taub.) Mendonca & E.P. Sousa phytochemical compounds on human α-glucosidases

Inhibitory andin silicomolecular docking ofXeroderris stuhlmannii(Taub.) Mendonca & E.P. Sousa phytochemical compounds on human α-glucosidases

Natural products for the treatment and management of diabetes mellitus in Zimbabwe-a review

Rotenoids and Isoflavones from Xeroderris stuhlmannii (Taub.) Mendonça & E.P. Souza and Their Biological Activities

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