Phytochemical Composition, Antifungal and Antioxidant Activity of <i>Duguetia furfuracea</i> A. St.‐Hill

Pinho, Francisca Valéria Soares de Araújo; Cruz, Litiele Cezar da; Rodrigues, Nathane Rosa; Waczuk, Emily Pansera; Souza, Celestina Elba Sobral de; Coutinho, Henrique Douglas Melo; Costa, José Galberto Martins da; Athayde, Margareth Linde; Boligon, Aline Augusti; Franco, Jeferson Luís; Posser, Thaís; Menezes, Irwin Rose Alencar de

doi:10.1155/2016/7821051

Cited by 17 publications

(12 citation statements)

References 41 publications

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“…These values are similar to those reported for pristimerin [ 50 ], with MICs ranging from 7.81 to 250 mg/L, and for the hexane extract of Annona glabra [ 56 ], which inhibited the growth of C. albicans at a concentration of 100 μg.mL -1 . The present study also showed that liriodenine has lower MIC values than the methanol and ethyl acetate extracts of Duguetia furfuracea against C. albicans and C. krusei , with MICs ≥ 1024 μg.mL -1 , although acting against C. tropicalis [ 57 ]. Liriodenine was also more active than FAMEs of Annona cornifolia , which exhibited MICs of 500 μg.mL -1 or higher against C. albicans and C .…”

Section: Discussionmentioning

confidence: 83%

Antifungal activity of liriodenine on agents of systemic mycoses, with emphasis on the genus Paracoccidioides

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De-la-Cruz-Chacón

González‐Esquinca

et al. 2020

J. Venom. Anim. Toxins incl. Trop. Dis

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Background: Endemic systemic mycoses remain a health challenge, since these opportunistic diseases are increasingly infecting immunosuppressed patients. The simultaneous use of antifungal compounds and other drugs to treat infectious or non-infectious diseases has led to several interactions and undesirable effects. Thus, new antifungal compounds should be investigated. The present study aimed to evaluate the activity of liriodenine extracted from Annona macroprophyllata on agents of systemic mycoses, with emphasis on the genus Paracoccidioides . Methods: The minimum inhibitory concentration (MIC) and minimum fungicide concentration (MFC) were determined by the microdilution method. The cellular alterations caused by liriodenine on a standard P. brasiliensis (Pb18) strain were evaluated by transmission and scanning electron microscopy. Results: Liriodenine was effective only in 3 of the 8 strains of the genus Paracoccidioides and in the Histoplasma capsulatum strain, in a very low concentration (MIC of 1.95 μg.mL -1 ); on yeasts of Candida spp. (MIC of 125 to 250 μg.mL-1), including C. krusei (250 μg.mL -1 ), which has intrinsic resistance to fluconazole; and in Cryptococcus neoformans and Cryptococcus gattii (MIC of 62.5 μg.mL -1 ). However, liriodenine was not effective against Aspergillus fumigatus at the studied concentrations. Liriodenine exhibited fungicidal activity against all standard strains and clinical isolates that showed to be susceptible by in vitro tests. Electron microscopy revealed cytoplasmic alterations and damage to the cell wall of P. brasiliensis (Pb18). Conclusion: Our results indicate that liriodenine is a promising fungicidal compound that should undergo further investigation with some chemical modifications.

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Section: Discussionmentioning

confidence: 83%

Antifungal activity of liriodenine on agents of systemic mycoses, with emphasis on the genus Paracoccidioides

Vinche

De-la-Cruz-Chacón

González‐Esquinca

et al. 2020

J. Venom. Anim. Toxins incl. Trop. Dis

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“…However, few chemical data are available on the Duguetia genus, despite its high number of species (Almeida et al, 2012). (Pinho et al, 2016;Sant os et al, 2018).…”

Section: Resultsmentioning

confidence: 99%

“…Flavonoids have the ability to induce apoptosis and block the cell cycle by demolishing the structure of the spindle fiber (Pumiputavon et al, 2017). Quercetin was demonstrated to be present in methanolic extract from D. furfuracea (Pinho et a., 2016), and this flavonoid can promote the proapoptotic gene Bax and activat e caspase pathways (Pumiputavon et al, 2017).…”

Section: Resultsmentioning

confidence: 99%

“…Many studies have been conducted on the secondary met abolites present in different parts of Duguetia plants, such as essential oils, aromatic compounds, monoterpenes, diterpenes, triterpenes, flavonoids, alkaloids and ac etogenins (Pérez & Cassels, 2010;Sousa et al, 2012;Rodrigues et al, 2015). Secondary metabolites are substances with a generally complex structure playing several roles in the adaptation of plants to the environment (Pinho et al, 2016). Nowadays, these substances have been attracting interest for t heir pharmacological potential, such as their antioxidant, anti-inflammatory, antimutagenic, anticarcinogenic, and antimicrobial properties (Pinho et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…Secondary metabolites are substances with a generally complex structure playing several roles in the adaptation of plants to the environment (Pinho et al, 2016). Nowadays, these substances have been attracting interest for t heir pharmacological potential, such as their antioxidant, anti-inflammatory, antimutagenic, anticarcinogenic, and antimicrobial properties (Pinho et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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In vitro cytotoxicity of Duguetia sp extracts on Ehrlich tumor cells

et al. 2020

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The genus Duguetia, of the Annonaceae family, includes around 100 species that have been used in folk medicine to treat several diseases. Its phytochemical compounds have been researched for their antifungal, antioxidant, antigenotoxic, anti-inflammatory and cytotoxic properties. Therefore, the objective of the present study was to conduct a preliminary study on the biological effects of Duguetia sp extracts by evaluating their in vitro cytoto xic effect on different cell types. To prepare the methanolic extract (ME), the leaves were macerated with 80% methanol. Part of the ME was dissolved in 10% phosphoric acid, and the acidic aqueous solution was partitioned with dichloromethane. The organic phase was evaporated to obtain an acetogenin-rich extract (ACE). The extracts were diluted in RPMI culture medium , supplemented with 20% fetal bovine serum , and added to Erhlich tumor cells or mice spleen cells at different concentrations: the ACE e xtract a t 1.35 mg mL-1 , 0.67 mg mL-1 or 0,34 mg mL-1 , and the ME e xtract at 1.40 mg mL-1 , 0.70 mg mL-1 or 0,35 mg mL-1. After 24 h, cytotoxicity analysis was performed using the Trypan Blue exclusion method. The results demonstrated that the extracts are cytotoxic at all concentrations to Ehrlich tumor cells and mice spleen cells. According to the results, we concluded that the extracts of Duguetia sp had a cytotoxic effect on the studied cells in vitro. This was the first study to report the biological effect of this plant genus in this type of cells; however, further study is needed to determine the current species used and the compounds present in the extracts.

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