Phytochemical constituents and biological activity of selected genera of family Crassulaceae: A review

Hassan, Marwa H. A.; Elwekeel, Ahlam; Moawad, Abeer; Afifi, Naglaa; Amin, Elham; Amir, Dalia El

doi:10.1016/j.sajb.2021.05.016

Cited by 18 publications

(9 citation statements)

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“…The Crassulaceae family belongs to the order Saxifragales, or the orpine family or Stonecrop family [13]. Regarding its distribution, the presence of members of the Crassulaceae family is documented in the Mediterranean region, Southern Africa, and the Southwestern United States [14], which includes 35 genera and ∼1410 species [15]. The genus Kalanchoe is widely recognized for its ornamental use, mainly attributed to its adaptability to drought, exquisite flowers, easy cultivation, clone growth, and asexual reproduction [16].…”

Section: Introductionmentioning

confidence: 99%

Biological Activities and Chemical Profiles of Kalanchoe fedtschenkoi Extracts

Mejía-Méndez

Bach

Lorenzo-Leal

et al. 2023

Plants

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In this study, the leaves of Kalanchoe fedtschenkoi were consecutively macerated with hexane, chloroform, and methanol. These extracts were used to assess the bioactivities of the plant. The antimicrobial activity was tested against a panel of Gram-positive and -negative pathogenic bacterial and fungal strains using the microdilution method. The cytotoxicity of K. fedtschenkoi extracts was investigated using human-derived macrophage THP-1 cells through the MTT assay. Finally, the anti-inflammatory activity of extracts was studied using the same cell line by measuring the secretion of IL-10 and IL-6. The phytoconstituents of hexane and chloroform extracts were evaluated using gas chromatography–mass spectrometry (GC/MS). In addition, high-performance liquid chromatography (HPLC) was used to study the phytochemical content of methanol extract. The total flavonoid content (TFC) of methanol extract is also reported. The chemical composition of K. fedtschenkoi extracts was evaluated using Fourier-transform infrared spectroscopy (FTIR). Results revealed that the chloroform extract inhibited the growth of Pseudomonas aeruginosa at 150 μg/mL. At the same concentration, methanol extract inhibited the growth of methicillin-resistant Staphylococcus aureus (MRSA). Regarding their cytotoxicity, the three extracts were highly cytotoxic against the tested cell line at IC50 < 3 μg/mL. In addition, the chloroform extract significantly stimulated the secretion of IL-10 at 50 μg/mL (p < 0.01). GC/MS analyses revealed that hexane and chloroform extracts contain fatty acids, sterols, vitamin E, and triterpenes. The HPLC analysis demonstrated that methanol extract was constituted by quercetin and kaempferol derivatives. This is the first report in which the bioactivities and chemical profiles of K. fedtschenkoi are assessed for non-polar and polar extracts.

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Section: Introductionmentioning

confidence: 99%

Biological Activities and Chemical Profiles of Kalanchoe fedtschenkoi Extracts

Mejía-Méndez

Bach

Lorenzo-Leal

et al. 2023

Plants

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“…To the best of our knowledge, this is the first study to investigate the antibacterial antiadherence properties of Echeveria plant extracts. Most of the studies evaluated the antibacterial properties of members of the Crassulaceae family and only determined the MIC; however, none of these studies evaluated whether the extracts had any effect in bacterial adherence [79] . Although effects on adherence were not observed in the present study, further analyses are needed to evaluate other possible mechanisms by which the Echeveria extracts could participate against bacterial infection.…”

Section: Discussionmentioning

confidence: 99%

Genomic characterization of a multidrug-resistant uropathogenic Escherichia coli and evaluation of Echeveria plant extracts as antibacterials

Castañeda-Meléndrez,

Magaña-Lizárraga,

Martínez-Valenzuela

et al. 2024

AIMSMICRO

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<abstract> Uropathogenic <italic>Escherichia coli</italic> (UPEC) is the most common bacterial agent associated with urinary tract infections, threatening public health systems with elevated medical costs and high morbidity rates. The successful establishment of the infection is associated with virulence factors encoded in its genome, in addition to antibacterial resistance genes, which could limit the treatment and resolution of the infection. In this sense, plant extracts from the genus <italic>Echeveria</italic> have traditionally been used to treat diverse infectious diseases. However, little is known about the effects of these extracts on bacteria and their potential mechanisms of action. This study aims to sequence a multidrug-resistant UPEC isolate (UTI-U7) and assess the multilocus sequence typing (MLST), virulence factors, antimicrobial resistance profile, genes, serotype, and plasmid content. Antimicrobial susceptibility profiling was performed using the Kirby-Bauer disk diffusion. The antibacterial and anti-adherent effects of the methanol extracts (ME) of <italic>Echeveria</italic> (<italic>E. craigiana</italic>, <italic>E. kimnachii</italic>, and <italic>E. subrigida</italic>) against UTI-U7 were determined. The isolate was characterized as an O25:H4-B2-ST2279-CH40 subclone and had resistant determinants to aminoglycosides, β-lactams, fluoroquinolones/quinolones, amphenicols, and tetracyclines, which matched with the antimicrobial resistance profile. The virulence genes identified encode adherence factors, iron uptake, protectins/serum resistance, and toxins. Identified plasmids belonged to the IncF group (IncFIA, IncFIB, and IncFII), alongside several prophage-like elements. After an extensive genome analysis that confirmed the pathogenic status of UTI-U7 isolate, <italic>Echeveria</italic> extracts were tested to determine their antibacterial effects; as an extract, <italic>E. subrigida</italic> (MIC, 5 mg/mL) displayed the best inhibitory effect. However, the adherence between UTI-U7 and HeLa cells was unaffected by the ME of the <italic>E. subrigida</italic> extract. </abstract>

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“…It has fleshy spatulate leaves, with a spine at the tip, and purplish edges [ 4 ]. The Crassulaceae family is morphologically diverse, with more than a thousand species, including succulent plants adapted to barren environments [ 5 , 6 ]. Orostachys species are distributed in numerous countries, including China, Korea, and Mongolia [ 1 ].…”

Section: Introductionmentioning

confidence: 99%

Profiling the Phytochemicals of Orostachys margaritifolia: Biological Activities, LC-ESI/MS, and HPLC Analyses

Yoon,

Yu,

Tran

et al. 2024

Pharmaceuticals

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Orostachys margaritifolia Y. N. Lee (OMY) is an endemic Korean plant in the family Crassulaceae that is known to contain a variety of bioactive compounds. To assess the physiological activities of an OMY ethanol extract, ABTS+ and DPPH radical scavenging assays and a nitric oxide (NO) inhibition assay were conducted. The phytochemical makeup of the extract was profiled via liquid chromatography-mass spectrometry (LC-ESI/MS) and high-performance liquid chromatography with a photodiode array detector (HPLC/PDA). The OMY extract was found to have weaker ABTS+ and DPPH radical scavenging activities than the control group (green tea). In the NO inhibition assay, the OMY extract induced a significant increase in macrophage cell viability but showed a lower NO inhibitory activity than l-NAME, producing an IC50 value of 202.6 μg/mL. The LC-ESI/MS and HPLC/PDA analyses identified isoquercitrin and astragalin in the OMY extract, quantifying their contents at 3.74 mg/g and 3.19 mg/g, respectively. The study revealed possibilities for the utilization of OMY as a future source of drugs for alleviating inflammation and diseases related to reactive oxygen species.

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Phytochemical constituents and biological activity of selected genera of family Crassulaceae: A review

Cited by 18 publications

References 100 publications

Biological Activities and Chemical Profiles of Kalanchoe fedtschenkoi Extracts

Biological Activities and Chemical Profiles of Kalanchoe fedtschenkoi Extracts

Genomic characterization of a multidrug-resistant uropathogenic <i>Escherichia coli</i> and evaluation of <i>Echeveria</i> plant extracts as antibacterials

Profiling the Phytochemicals of Orostachys margaritifolia: Biological Activities, LC-ESI/MS, and HPLC Analyses

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