Phytochemical Content and Pharma‐Nutrition Study on Eleutherococcus senticosus Fruits Intractum

Abstract

In the past two decades public interest in herbal products has increased significantly in Europe, especially in the plant-based products from non-European traditions. Eleutherococcus senticosus has been used for the treatment of inflammatory diseases, anemia, and rheumatoid arthritis. The Eleutherococcus senticosus fruits intractum was examined for the content of phenolic acids (LC-ESI-MS/MS), minerals (AAS), TPC, and TFC (spectrophotometric assay). The antioxidant activity was determined using free radical scavenging assay and TLC-DB-DPPH∗ dot-blot test. An anti-Hyal activity was evaluated by the spectrophotometric assay method. Cytotoxicity towards HL-60, HL-60/MX1, HL-60/MX2, CEM/C1, and CCRF/CEM leukemic cell lines was done using trypan blue test. Among eight phenolic acids, trans-caffeic acid was found in the largest amount (41.2 mg/g DE). The intractum presented a high amount of macroelements (Ca, Mg, K; 1750, 1300, and 21000 mg/kg) and microelements (Fe, Mn; 32.7, 54.3 mg/kg), respectively. The content of TPC and TFC was 130 and 92 mg/g DE, respectively. The intractum showed anti-Hyal activity (2.16–60%) and an antioxidant capacity (EC50; 52 μg/mL). The intractum most strongly inhibited the growth of HL-60, HL-60/MX1, and CCRF/CEM. A better understanding of the intractum health benefits is important in order to increase its utility and enrich dietary sources of health promoting compounds.

EleutherococcusSpecies Cultivated in Europe: A New Source of Compounds with Antiacetylcholinesterase, Antihyaluronidase, Anti-DPPH, and Cytotoxic Activities

“…In Załuski et al's previous studies, aescin was used as the standard compound. The 30.1% level of inhibition was obtained for the concentration of 100 μ g [ 12 ]. It should be noticed that the extract possesses chemical structures that may be used as possible inhibitors of Hyal, showing a stronger potency than aescin.…”

“…Phenolic acid profile was determined according to the modified methods of Nowacka et al, [ 19 ], Pietrzak et al, [ 20 ], and Załuski et al, [ 12 ]. In this case, the Agilent 1200 Series HPLC system (Agilent Technologies, USA) connected to a 3200 QTRAP MS/MS (AB Sciex, USA) was used.…”

“…In the literature, there is no information on an antihyaluronidase (anti-Hyal) activity of Eleutherococcus spp., apart from Załuski et al's reports [6, 12]. Hyaluronidase is the enzyme that takes part in tumor invasiveness and the development of inflammation.…”

Brief Challenges on Medicinal Plants: An Eye‐Opening Look at Ageing‐Related Disorders

“…It has also been reported that Siberian ginseng is an effective scavenger of peroxynitrite (ONOO − ), while to a Siberian ginseng MeOH extract was found the maximum ferric reducing antioxidant power (FRAP) . Also, it has been utilized in the treatment of anaemia, inflammatory disorders and rheumatoid arthritis .…”

Phytoconstituents and Nutritional Properties of the Fruits of Eleutherococcus divaricatus and Eleutherococcus sessiliflorus: A Study of Non‐European Species Cultivated in Poland

“…With regard to its pharmacological aspects, E. sessiliflorus has been used in traditional medical protocols as a tonic, analgesic, antihypertensive, and antidiabetic agent. Załuski's previous studies have shown that the fruits may reduce DPPH ∗ radical, inhibit lipid peroxidation, and have an ion-chelating ability [ 6 – 10 ]. In food industry, only species native to Asia are used, while species cultivated in Europe are not yet used commercially.…”