Phytochemical Investigation, Antioxidant Properties and In Vivo Evaluation of the Toxic Effects of Parthenium hysterophorus

Iqbal, Javed; Khan, Ayaz Ali; Aziz, Tariq; Ali, Waqar; Ahmad, Saeed; Rahman, Shafiq Ur; Iqbal, Zafar; Dablool, Anas S.; Alruways, Mashael W.; Almalki, Abdulraheem Ali; Alamri, Abdulhakeem S.; Alhomrani, Majid

doi:10.3390/molecules27134189

Cited by 12 publications

(9 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, the binding energy of standard drug was also higher than the C. paradisi leaf components such as geraniol and betapinene. The protein TRPV4 is selected as analgesic protein and it is confirmed by previous literature (22,(47)(48)(49)(50)(51)(52)(53). The docking of TRPV4 was done with 2 ligand molecules (geraniol and beta-pinene) and diclofenac is used as standard drug.…”

Section: Selected Amino Acids For 3 Proteinsmentioning

confidence: 74%

In-Vivo AND In-Silico ANALYSIS OF ANTI- INFLAMMATORY, ANALGESIC, AND ANTI PYRETIC ACTIVITIES OF In-Silico LEAF EXTRACT

Zahra,

Fareed,

Hamza

et al. 2023

J. Chil. Chem. Soc.

View full text Add to dashboard Cite

Medicinal plants recently gained attention due to the presence of many phytochemicals involved in various therapeutical activities. The main aim of this study was to determine the in-vivo and in-silico anti-inflammatory, analgesic, and anti-pyretic effects of Citrus paradisi leaf ethanolic extract using albino rats (n = 36). For inducing inflammation, pain, and fever in albino rat's carrageenan, acetic acid dilution in distilled water and yeast dilution in saline were used. The four different concentrations (50, 100, 200, and 400mg/kg) of ethanolic extract of Citrus paradisi leaf were used to prevent inflammation, pain, and fever. Diclofenac and paracetamol were used as standard drugs in this study. The ethanolic extract of Citrus paradisi leaf showed efficient anti-pyretic and anti-inflammatory inhibition (90% and 80%, respectively) but less efficient analgesic inhibition (36%). Similarly, in-silico study was done using leaf bioactive compounds such as linalool, beta-pinene, geraniol, citral, and terpinene-4-ol as ligand molecules and proteins for anti-inflammatory, analgesic, and anti-pyretic activity were PTGS2, TRPV4, and TLR2, respectively. The process of docking was done using ligand and protein molecules. The results of in-silico study were the same as in-vivo study; the binding energy values of anti-inflammatory and anti-pyretic activity were more efficient than an analgesic. In summary, the ethanolic extract of Citrus paradisi leaf in in-silico and in-vivo studies proved less efficient against pain while more efficient against inflammation and fever.

show abstract

Section: Selected Amino Acids For 3 Proteinsmentioning

confidence: 74%

In-Vivo AND In-Silico ANALYSIS OF ANTI- INFLAMMATORY, ANALGESIC, AND ANTI PYRETIC ACTIVITIES OF In-Silico LEAF EXTRACT

Zahra,

Fareed,

Hamza

et al. 2023

J. Chil. Chem. Soc.

View full text Add to dashboard Cite

show abstract

“…The phenolic compounds such as chlorogenic acid found in MEPH has hypoglycemic potential by inhibiting glucose-6-phosphate translocase that otherwise transports glucose-6-phosphate into the lumen of the endoplasmic reticulum to hydrolyze it into glucose. Similarly, ellagic acid and alkaloids also inhibit the glucose-1-phosphate, a key enzyme of glycogenolysis (23).…”

Section: Discussionmentioning

confidence: 99%

Antidiabetic and pancreas-protective potential of Parthenium Hysterophorous (Carrot grass) in Alloxan induced diabetic rabbits

Makhdoom¹,

Maqbool²,

Muhammad³

et al. 2022

Cell Mol Biol (Noisy-le-grand)

View full text Add to dashboard Cite

“…The qualitative and quantitative analysis carried out on active fractions of methanolic extract, especially isolated compounds for screening of phytochemicals [32] …”

Section: Methodsmentioning

confidence: 99%

Characterization of Secondary Metabolites Identified in Lentinula edodes and Determination of Their Biological Activities by in‐silico Antioxidant Screening

Amin,

Afzal,

Riaz

2023

ChemistrySelect

View full text Add to dashboard Cite

Mushrooms, like other fungi, are unique in the living world They have been assigned to the kingdom of Myceteae. Lentinula edodes(shiitake), is second‐largest and most often consumed edible mushroom in the world. Shiitake mushroom possess large capability to be used as food as it contains carbohydrates, proteins, vitamins, minerals, fibers, fatty acids, amino acids, etc. In current study powdered form mushroom used to form semisolid extract in selected fractions (Butanol, ethyl acetate, hexane, chloroform and aqueous). The aim of the current study was to explore phytoconstituents which have biological activities in selected fractions (n. hexane chloroform, and ethyl acetate) of methanolic extract of Lentinula edodes.GC‐MS, and HPLC analysis presented the secondary metabolites in all the selected fractions under taken. Chloroform fraction had the highest total phenolic, and total flavonoid which exhibited the maximum in vitro antioxidant ABTS potential (74.78±0.83) potential at 350 μg/mL while ethyl acetate has moderate and n‐hexane shows least concentration. Hemolysis essay showed less than 10 % lysis of RBC, s which indicates that fractions under study are active, and safe to use as herbal medicine. Biomolecules detected through HPLC and GC‐MS in fractions under study showed significant inhibition in paw inflammation (1.18±0.06) and yeast induced pyrexia (36.8±0.68) in dose dependent manner. Furthermore, In‐silico study showed that 4H‐Furo[3,2‐b] pyrrole‐5‐carboxylic acid have a strong interaction to NADPH oxidase, Myeloperoxidase as compared to standard Melatonin which shows best antioxidant compound which was confirmed in chloroform fraction.The outcomes of this research indicated that L. edodes significantly reduced inflammation and have ability to scavenge free radicals, prevent protein denaturation, also indicate the possible use in food and nutraceutical applications as an antioxidant, antipyretic, anti‐inflammatory, and antidiabetic agent.

show abstract

Phytochemical Investigation, Antioxidant Properties and In Vivo Evaluation of the Toxic Effects of Parthenium hysterophorus

Cited by 12 publications

References 41 publications

In-Vivo AND In-Silico ANALYSIS OF ANTI- INFLAMMATORY, ANALGESIC, AND ANTI PYRETIC ACTIVITIES OF In-Silico LEAF EXTRACT

In-Vivo AND In-Silico ANALYSIS OF ANTI- INFLAMMATORY, ANALGESIC, AND ANTI PYRETIC ACTIVITIES OF In-Silico LEAF EXTRACT

Antidiabetic and pancreas-protective potential of Parthenium Hysterophorous (Carrot grass) in Alloxan induced diabetic rabbits

Characterization of Secondary Metabolites Identified in Lentinula edodes and Determination of Their Biological Activities by in‐silico Antioxidant Screening

Contact Info

Product

Resources

About