Phytochemical mediated gold nanoparticles and their PTP 1B inhibitory activity

Basha, Sabjan Khaleel; Govindaraju, K.; Ramesh, Manikandan; Ahn, Jong Seog; Bae, Eun Young; Ganesan, Singaravelu

doi:10.1016/j.colsurfb.2009.09.008

Cited by 70 publications

(26 citation statements)

References 32 publications

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“…In vitro stability study was performed as described by Basha et al with modification [16]. Briefly, 0.5 mL of AuNP suspension were added to 0.5 mL of 0.5% HSA, phosphate buffer saline at pH 5.0, 7.4, and 9.0, DMEM media, and DMEM with 10% FBS, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…In recent years, the green synthesis of AuNPs has emerged as an alternative method due to its simplicity, cost, and energy effectiveness and considerable reduction in the generation of hazardous waste [13]. Green synthesis utilizes natural biomolecules such as polysaccharides [14], microbial enzymes [15], and phytochemicals [16] as the reducing and stabilizing agents in the formation of AuNPs. In contrast to physical and chemical synthesis, this process normally does not require heat or pressure, which ultimately reduces the energy usage and therefore lowers the production cost [17].…”

Section: Introductionmentioning

confidence: 99%

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Curcuma mangga-Mediated Synthesis of Gold Nanoparticles: Characterization, Stability, Cytotoxicity, and Blood Compatibility

et al. 2017

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The utilization of toxic chemicals as reducing and stabilizing agents in the preparation of gold nanoparticles (AuNPs) has increased in vivo toxicity and thus limited its application in clinical settings. Herein, we propose an alternative method of preparing highly stable AuNPs, where non-toxic Curcuma mangga (CM) extract was used as a single reducing and stabilizing agent to overcome the aforementioned constraints. The morphological images enunciated that the homogeneously dispersed AuNPs exhibited spherical morphology with an average particle diameter of 15.6 nm. Fourier Transform infrared (FTIR) and cyclic voltammetry analysis demonstrated that carbonyl groups of terpenoids in CM extract played an important role in the formation and stabilization of AuNPs. Green-synthesized AuNPs were found to have good stability in physiological media after 24 h of dispersion. The AuNPs were also cytocompatible with human colon fibroblast cell (CCD-18Co) and human lung fibroblast cell (MRC-5). Hemocompatibility tests revealed that the AuNPs were blood-compatible, with less than 10% of hemolysis without any aggregation of erythrocytes. The current study suggests potential in employing a CM-extract-based method in the preparation of AuNPs for anticancer diagnosis and therapy.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Curcuma mangga-Mediated Synthesis of Gold Nanoparticles: Characterization, Stability, Cytotoxicity, and Blood Compatibility

et al. 2017

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“…The methanol extract of guava leaves was found to be the most efficient in lowering glucose levels. Basha et al [64] demonstrated the ability of guavanoic-acid-mediated gold nanoparticles to inhibit the protein tyrosine phosphatase 1B activity.…”

Section: Pharmacological Propertiesmentioning

confidence: 99%

Health Effects of Psidium guajava L. Leaves: An Overview of the Last Decade

Díaz‐de‐Cerio

Verardo

Gómez-Caravaca

et al. 2017

IJMS

142

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Today, there is increasing interest in discovering new bioactive compounds derived from ethnomedicine. Preparations of guava (Psidium guajava L.) leaves have traditionally been used to manage several diseases. The pharmacological research in vitro as well as in vivo has been widely used to demonstrate the potential of the extracts from the leaves for the co-treatment of different ailments with high prevalence worldwide, upholding the traditional medicine in cases such as diabetes mellitus, cardiovascular diseases, cancer, and parasitic infections. Moreover, the biological activity has been attributed to the bioactive composition of the leaves, to some specific phytochemical subclasses, or even to individual compounds. Phenolic compounds in guava leaves have been credited with regulating blood-glucose levels. Thus, the aim of the present review was to compile results from in vitro and in vivo studies carried out with guava leaves over the last decade, relating the effects to their clinical applications in order to focus further research for finding individual bioactive compounds. Some food applications (guava tea and supplementary feed for aquaculture) and some clinical, in vitro, and in vivo outcomes are also included.

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“…The phytochemical guavanoic acid reduced gold nanoparticles inhibits PTP1B activity in vitro . This explains the modulating action of phytochemical reduced gold nanoparticles at the level of post receptor signaling molecules like PTP1B.…”

Section: Discussionmentioning

confidence: 91%

“…It has been suggested that the plant secondary metabolites serve as excellent bioreductants for the synthesis of metallic nanoparticles that display different biological actions. For example, a phytochemical, guavanoic acid reduced gold nanoparticles inhibits Protein Tyrosine Phosphatase‐1B (PTP1B) in vitro . The bioreduced gold nanoparticles display anti‐inflammatory, antioxidant, and antihyperglycemic activities …”

Section: Introductionmentioning

confidence: 99%

Biogenesis, characterization, and the effect of vicenin‐gold nanoparticles on glucose utilization in 3T3‐L1 adipocytes: A bioinformatic approach to illuminate its interaction with PTP 1B and AMPK

Chockalingam¹,

Thada²,

Dhandapani³

et al. 2015

Biotechnology Progress

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This study reported the synthesis of Vicenin-2 gold nanoparticles (VN-AuNPs) and evaluated their effect on the glucose utilization efficiency of 3T3-L1 adipocytes. The VN-AuNPs were characterized by microscopic, DLS and spectral analysis. The bio-reducing efficiency of Vicenin-2 (VN) was computed and confirmed by HPLC analysis. The stability of VN-AuNPs in various physiological media was explored. The cytotoxicity and glucose uptake assays were performed in 3T3-L1 adipocytes. The docking of VN with PTP1B and AMPK was also performed. The color change and UV absorption at 537 nm preliminarily confirmed the VN reduced gold nanoparticles. The VN-AuNPs appeared as spherical particles (57 nm) and face centered cubic crystals under TEM and XRD analysis, respectively. Its zeta potential was found to be -6.53 mV. The FT-IR spectra of VN and its AuNPs confirmed its stability. The computed reducing potential of VN was similar to the extent of VN utilized during the synthesis of VN-AuNPs. The VN-AuNPs showed a remarkable stability in different physiological media. At 100 µM concentration, VN-AuNPs displayed 78.21% cell viability. A concentration dependent increase in glucose uptake was noted in 3T3-L1 adipocytes when incubated with VN-AuNPs. The docking data revealed a strong interaction of VN with the binding pockets of PTP1B and AMPK. This demonstrates that the fabricated VN-AuNPs might enhance the intracellular VN availability mediated cellular glucose utilization and this would serve as a novel nanodrug for the management of diabetes.

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Phytochemical mediated gold nanoparticles and their PTP 1B inhibitory activity

Cited by 70 publications

References 32 publications

Curcuma mangga-Mediated Synthesis of Gold Nanoparticles: Characterization, Stability, Cytotoxicity, and Blood Compatibility

Curcuma mangga-Mediated Synthesis of Gold Nanoparticles: Characterization, Stability, Cytotoxicity, and Blood Compatibility

Health Effects of Psidium guajava L. Leaves: An Overview of the Last Decade

Biogenesis, characterization, and the effect of vicenin‐gold nanoparticles on glucose utilization in 3T3‐L1 adipocytes: A bioinformatic approach to illuminate its interaction with PTP 1B and AMPK

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