Phytochemical profile and larvicidal properties of seed essential oil from Nigella sativa L. (Ranunculaceae), against Aedes aegypti, Anopheles stephensi, and Culex quinquefasciatus (Diptera: Culicidae)

Raj, G. Adaikala; Chandrasekaran, M.; Krishnamoorthy, Sujatha; Jayaraman, M.; Venkatesalu, V.

doi:10.1007/s00436-015-4563-3

Cited by 28 publications

(11 citation statements)

References 38 publications

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“…The larvicidal activities of the essential oil of Nigella sativa after 24 h of exposure period were reported to be higher (LC 50 53.9 ppm, LC 90 172.6 ppm) for A. stephensi 33 and while those for thyme oil nanoemulsion (LC 50 —18.88 ppm and LC 90 —95.23 ppm) and its encapsulation (LC 50 —56.13 ppm and LC 90 —124.38 ppm) remarkably lower lethal concentrations were obtained in the present study for the same test organism and same time period. The χ 2 value thyme oil nanoemulsion and its encapsulation showed a significant decrease of 2.96 and 2.51 from the 9.2 of the essential oil of N. sativa .…”

Section: Discussionmentioning

confidence: 99%

Preparation of Thymus vulgaris (L.) essential oil nanoemulsion and its chitosan encapsulation for controlling mosquito vectors

Gupta

Preet

Ananya

et al. 2022

Sci Rep

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Here, we report a novel comparative assessment of preparation and characterization of thyme oil nanoemulsion and its chitosan encapsulation using high energy approach for the management of three major mosquito species viz., Anopheles stephensi (Liston,1901), Aedes aegypti (Linn., 1762) and Culex tritaeniorhynchus (Giles, 1901). The synthesized formulations were analysed for thermodynamic stability, indicating 1:0.5 (oil: surfactant) ratio to be the most stable of thyme oil nanoemulsion while 1:1 (nanoemulsion: chitosan solution) ratio of its chitosan encapsulation. These were further characterized by dynamic light scattering and transmission electron microscopy which revealed the size and morphology of the droplets which measured 52.18 ± 4.53 nm for thyme oil nanoemulsion and 50.18 ± 2.32 nm for its chitosan encapsulation. All the droplets were well dispersed with distinct flower-shaped nanoemulsion and somewhat mitochondria like chitosan encapsulation. In-vitro release study of thyme essential oil from its nanoemulsion and chitosan encapsulation showed that 91.68% and 73.41% of the total oil concentration in water was released respectively to the environment after 48 h clearly depicting controlled release in the encapsulation. Assessment of insecticidal potential against selected mosquito species revealed that both the nanoemulsion and its chitosan encapsulation were effective on the major mosquito species. Maximum activity of thyme oil nanoemulsion was noticed against C. tritaeniorhynchus (LC50—22.58 ppm) after 24 h of exposure while it was observed that its chitosan encapsulation was most effective on A. stephensi (LC50—18.88 ppm) after 24 h of exposure. Consistent morphological alterations could be noticed in the larvae of mosquito species. Hence, these nanoemulsions and encapsulations could be further tested for their applications against other insect pests in agriculture.

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Section: Discussionmentioning

confidence: 99%

Preparation of Thymus vulgaris (L.) essential oil nanoemulsion and its chitosan encapsulation for controlling mosquito vectors

Gupta

Preet

Ananya

et al. 2022

Sci Rep

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“…Thymoquinone (TQ) was found to be the main bioactive constituents of the volatile oil of the black seed (54 %) (Abou Basha et al 1995). Recently, it has been proven to be a natural larvicidal agent against the fourth instar larvae of Aedes aegypti, Anopheles stephensi, and Culex quinquefasciatus (Raj et al 2015).…”

Section: Discussionmentioning

confidence: 99%

Effect of Nigella sativa oil on experimental toxoplasmosis

2015

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Toxoplasmosis is a parasitic infection caused by Toxoplasma gondii protozoon. It is most commonly treated by pyrimethamine (PYR); however, this was intolerable by many patients. The aim of this study was to assess therapeutic effects of Nigella sativa oil (NSO) alone and combined with pyrimethamine (PYR) compared to a previous combination of clindamycin (CLN) and (PYR). One hundred Albino mice were used in the current study and were equally divided into five groups: normal (I), infected untreated control (II); infected, treated with NSO-only (III); infected, treated with NSO + PYR (IV); and infected, treated with CLN + PYR (V). The virulent RH Toxoplasma strain was used in infection survival rates estimation, impression smears from liver and spleen, and histopathological and ultrastructural studies were done. Liver malondialdehyde (MDA) level and total antioxidant capacity (TAC) were determined. Interferon-γ and specific IgM were also measured in sera by ELISA. Results showed that NSO alone has no direct anti-Toxoplasma effect, whereas its combination with PYR produced potent effect that is comparable to CLN + PYR. It significantly increased the survival rate and decreased the parasite density and pathological insult in both liver and spleen. Also, significant increase in interferon-γ level denotes stimulation of cellular immunity. NSO + PYR combination markedly improved the antioxidant capacity of Toxoplasma infected mice compared to the infected untreated ones and to CLN/PYR. In conclusion, although NSO, if administered alone, has significant immunostimulant and antioxidant properties, it failed to decrease tachyzoite counts. Combination of NSO and PYR had synergistic effect in treatment of toxoplasmosis.

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“…Recently, plant-sourced essential oils have attracted much attention from researchers and have been studied extensively. Raj GA et al 7 studied the chemical constituents of the essential oil from the seeds of Nigella sativa and the larvicidal activity against Aedes aegypti, A. stephensi, and Culex quinquefasciatus. The essential oils from salvia, Artemisia nilagirica, Tanacetum argenteum, and Rosmarinus officinalis also showed larvicidal activity against mosquitoes [8][9][10][11] .…”

Section: /5mentioning

confidence: 99%

Chemical composition and larvicidal activities of essential oil of Cinnamomum camphora (L.) leaf against Anopheles stephensi

Qin

Wang

et al. 2020

Rev. Soc. Bras. Med. Trop.

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Introduction: Anopheles stephensi is the main malaria vector in Southeast Asia. Recently, plant-sourced larvicides are attracting great interests. Methods: The essential oil was extracted from the leaf of Cinnamomum camphora (L.), and a bioassay was conducted to determine the larvicidal efficacy. The chemical composition of the essential oil was determined by GC-MS analysis. Results: The oil showed strong, dose-dependent larvicidal activities. The onset of larvicidal efficiency was rapid. The LC50 and LC95 were determined as 0.146% and 1.057% at 1 h, 0.031% and 0.237% at 12 h, 0.026% and 0.128% at 24 h, respectively. The oil contains 32 compounds. Conclusions: The essential oil of C. camphora leaf has an excellent larvicidal potential for the control of A. stephensi.

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Phytochemical profile and larvicidal properties of seed essential oil from Nigella sativa L. (Ranunculaceae), against Aedes aegypti, Anopheles stephensi, and Culex quinquefasciatus (Diptera: Culicidae)

Cited by 28 publications

References 38 publications

Preparation of Thymus vulgaris (L.) essential oil nanoemulsion and its chitosan encapsulation for controlling mosquito vectors

Preparation of Thymus vulgaris (L.) essential oil nanoemulsion and its chitosan encapsulation for controlling mosquito vectors

Effect of Nigella sativa oil on experimental toxoplasmosis

Chemical composition and larvicidal activities of essential oil of Cinnamomum camphora (L.) leaf against Anopheles stephensi

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