Phytochemical Profiles and Antioxidant Activity of Rehmannia glutinosa from Different Production Locations

Abstract

The chemical components and antioxidant activity of 16 Rehmannia glutinosa samples were investigated to reveal the high‐quality raw resource for pharmaceutical products. 22 main chemical components were detected with significant content differences (P<0.05). The contents of 14 substances reached the maximum in S1 sample such as catalpol (6.74 mg g−1), rehmaionoside A (1.93 mg g−1) and rehmannioside D (5.13 mg g−1). However, the content distribution of the other eight substances had no obvious change regulation. Three antioxidant evaluation methods commonly showed that S1 sample had strong antioxidant activity with a low IC50 value of 0.022 mg mL−1, a high ABTS value of 524.196 μmol equiv. Trolox g−1, and a high FRAP value of 200.517 μmol equiv. Trolox g−1. Considered the medicinal value, S1 had high quality based on the present phytochemical profiles and antioxidant activity. These results also indicated that the root extracts of R. glutinosa could become useful supplement for pharmaceutical products as new antioxidant agents.

Major Fatty Acid Profiles and Bioactivity of Seed Oils from Ten Tree Peony Cultivars as a Potential Raw Material Source for the Cosmetics and Healthy Products

“…The DPPH IC 50 value is a sample concentration, at which DPPH is scavenged by 50 %, and a lower IC 50 value represents a stronger DPPH radical scavenging activity. The method of decolorization of free radical ABTS + was performed as expressed previously [33,34] . The results were expressed as micromoles of Trolox equivalents (TE) per 1 g of dry weight (μmol TE g −1 ).…”

“…The method of decolorization of free radical ABTS + was performed as expressed previously. [33,34] The results were expressed as micromoles of Trolox equivalents (TE) per 1 g of dry weight (μmol TE g À 1 ). All determinations were carried out in triplicate.…”

Phenolics, Flavonoids Content and Antioxidant Activities of Tuber indicum at Different Maturity Stages

“…This assay based on the oxidation reduction reaction as the same as DPPH assay is to evaluate the antioxidant activity by measuring the reduction rate of 2,29-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS * + ). [18,21] It is obvious that the scavenging rate of ABTS * + increased with an increase in the concentration and found to be maximum at the highest concentration (Figure 2). In addition, ABTS * + scavenging activity of test samples at different maturity stages represented significant differences (P < 0.05) (Table 2).…”

“…[19] A lower EC 50 value can represent a stronger DPPH * scavenging activity. [20,21] In our study, the EC 50 value calculated by GraphPad Prism based on the raw data was used for a more intuitive comparison of the antioxidant activity of T. indicum at different maturity stages. For the samples at different maturity stages, the EC 50 values ranged from 1.13 mg/mL to 2.62 mg/mL, which further demonstrated antioxidant activity of the test samples had the significant differences (P < 0.05) (Table 2).…”

“…In addition, a high content of one or some active components in plants and mushrooms does not necessarily have a strong antioxidant activity, which may be due to the synergism of various natural chemical constituents. [21] Therefore, the composition and structure of active ingredients should be further analyzed and the antioxidant activity of T. indicum in cells and experimental animals should be further explored by detecting oxidative DNA damage, mitochondrial damage and protein damage. It is also vitally important for us to analyze other antioxidant substances, such as protein, polysaccharide, and trace elements, and explore their effects on antioxidant activity at different maturity stages in the future.…”