Phytochemical Prospection and Modulation of Antibiotic Activity In Vitro by<i>Lippia origanoides</i>H.B.K. in Methicillin Resistant<i>Staphylococcus aureus</i>

Barreto, Humberto Medeiros; Fontinele, Filipe Cerqueira; Oliveira, Aldeídia Pereira de; Arcanjo, Daniel Dias Rufino; Santos, Bernadete Helena Cavalcanti dos; Abreu, Aislan Pereira Lira de; Coutinho, Henrique Douglas Melo; Silva, Romézio Alves Carvalho da; Sousa, Taciana Oliveira de; Medeiros, Maria das Graças Freire de; Citó, Antônia Maria das Graças Lopes; Lopes, José Arimateia Dantas

doi:10.1155/2014/305610

Cited by 27 publications

(10 citation statements)

References 42 publications

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“…Thymol and carvacrol have been shown to have antimicrobial, antigenotoxic and anti-protozoal properties, and may therefore be responsible for the bioactive effects of L. origanoides essential oil (Nostro et al, 2004;Sarrazin et al, 2015aSarrazin et al, , 2015bVicuña et al, 2010;Escobar et al, 2010). However, bioactivity may also be due to a synergism of the chemical components of the essential oil (Barreto et al, 2014a).…”

Section: Discussionmentioning

confidence: 99%

“…Lippia origanoides Kunth (Verbenaceae), is an aromatic shrub found from Southern North America, through Central America, to the north of repellents (Oliveira et al, 2007;Escobar et al, 2010;Vicuña et al, 2010;Betancourt et al, 2012;Caballero-Gallardo et al, 2012;Barreto et al, 2014aBarreto et al, , 2014bSarrazin et al, 2015aSarrazin et al, , 2015bTeles et al, 2014;Vera et al, 2014). However, to the best of our knowledge, there are no existing studies exploring the potential of the essential oil of L. origanoides as a treatment against parasites in fish.…”

Section: Introductionmentioning

confidence: 99%

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Antiparasitic, physiological and histological effects of the essential oil of Lippia origanoides (Verbenaceae) in native freshwater fish Colossoma macropomum

et al. 2017

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This study examined the in vitro and in vivo, histopathological, anti-parasitic and hematopathological effects of the essential oil of Lippia origanoides on Colossoma macropomum. Essential oil concentrations of 10, 20, 40, 80, 160 and 320 mg•L −1 were tested in vitro against monogenoideans (Anacanthorus spathulatus, Notozothecium janauachensis and Mymarothecium boegeri) from the gills of C. macropomum. Concentrations of 320 and 160 mg•L −1 were 100% effective against these parasites within 20 and 60 min of exposure, respectively. The 80 mg•L −1 concentration was approximately 80% effective with 3 h of exposure, reaching 100% with 6 h of exposure. The 40 mg•L −1 concentration was also 100% effective with 6 h of exposure. The other concentrations were only weakly effective in vitro. Parasite mortality in controls (water or water + alcohol) began after 3 h, with 100% mortality after 8 h. In vivo tests, in which fry of C. macropomum were placed in baths with 20 mg•L −1 of the essential oil for 60 min, and 40 mg•L −1 for 30 min, did not lead to reductions in parasite abundances. In addition, the essential oil had an anaesthetic effect on fish, increased total protein levels, increased monocyte and neutrophil numbers, and reduced haematocrit. Slight to moderate and severe damage was observed in the gills of C. macropomum fingerling immediately after exposure to the essential oil, and 24 h after the treatments were applied, with no difference between treatments. Histological changes observed in the gills after exposure to concentrations of 20 and 40 mg•L −1 of L. origanoides essential oil were: hyperplasia and fusion of the lamellar epithelium, capillary dilation, displacement of the lamellar epithelium, and lamellar aneurism and epithelial rupturing with haemorrhaging. Oedema, mucous and chloride cell proliferation, lamellar hypertrophy, congestion and necrosis were less frequently observed. It can be concluded that the essential oil of L. origanoides was dose-dependent in vitro effect against monogenoidean parasites of C. macropomum. Unfortunately, the low concentrations tolerated by the fish in the vivo assay (20 and 40 mg•L −1) was not effective. Statement of relevance: The manuscript represents original research on use of the essential oil of Lippia origanoides against ectoparasites of Colossoma macropomum, an important finfish of Amazon region. This manuscript includes treatment in vitro against monogenoideans, and in vivo against protozoans and monogenoideans. Besides, histopathological and hematological analysis of the fish exposed to different concentrations of L. origanoides, a medical plant from North, Central and South America, were performed.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antiparasitic, physiological and histological effects of the essential oil of Lippia origanoides (Verbenaceae) in native freshwater fish Colossoma macropomum

et al. 2017

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“…Moreover, the addition of 128 μg/mL of L. origanoides EO to the growth medium did cause a 10-fold decrease in the MIC of neomycin (2500–248 μg/mL) and amikacin (788–78 μg/mL). This demonstrated a synergistic effect between L. origanoides EO and aminoglycosides against the methicillin resistant Staphylococcus aureus (MRSA) strain [ 46 ].…”

Section: Antimicrobial Synergismmentioning

confidence: 99%

Antimicrobial Activity and Chemical Composition of Essential Oils from Verbenaceae Species Growing in South America

2018

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The Verbenaceae family includes 2600 species grouped into 100 genera with a pantropical distribution. Many of them are important elements of the floras of warm-temperature and tropical regions of America. This family is known in folk medicine, and its species are used as digestive, carminative, antipyretic, antitussive, antiseptic, and healing agents. This review aims to collect information about the essential oils from the most reported species of the Verbenaceae family growing in South America, focusing on their chemical composition, antimicrobial activity, and synergism with commercial antimicrobials. The information gathered comprises the last twenty years of research within the South American region and is summarized taking into consideration the most representative species in terms of their essential oils. These species belong to Aloysia, Lantana, Lippia, Phyla, and Stachytarpheta genera, and the main essential oils they contain are monoterpenes and sesquiterpenes, such as β-caryophyllene, thymol, citral, 1,8-cineole, carvone, and limonene. These compounds have been found to possess antimicrobial activities. The synergism of these essential oils with antibiotics is being studied by several research groups. It constitutes a resource of interest for the potential use of combinations of essential oils and antibiotics in infection treatments.

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“…The various therapeutic advantages of compounds sourced from nature have been reviewed [ 8 , 9 , 10 , 11 ]. In addition, about 80% of antibiotics that are recently approved for treating many life-threatening infections are sourced from natural products (NPs) [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Cheminformatic Profiling and Hit Prioritization of Natural Products with Activities against Methicillin-Resistant Staphylococcus aureus (MRSA)

2021

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Several natural products (NPs) have displayed varying in vitro activities against methicillin-resistant Staphylococcus aureus (MRSA). However, few of these compounds have not been developed into potential antimicrobial drug candidates. This may be due to the high cost and tedious and time-consuming process of conducting the necessary preclinical tests on these compounds. In this study, cheminformatic profiling was performed on 111 anti-MRSA NPs (AMNPs), using a few orally administered conventional drugs for MRSA (CDs) as reference, to identify compounds with prospects to become drug candidates. This was followed by prioritizing these hits and identifying the liabilities among the AMNPs for possible optimization. Cheminformatic profiling revealed that most of the AMNPs were within the required drug-like region of the investigated properties. For example, more than 76% of the AMNPs showed compliance with the Lipinski, Veber, and Egan predictive rules for oral absorption and permeability. About 34% of the AMNPs showed the prospect to penetrate the blood–brain barrier (BBB), an advantage over the CDs, which are generally non-permeant of BBB. The analysis of toxicity revealed that 59% of the AMNPs might have negligible or no toxicity risks. Structure–activity relationship (SAR) analysis revealed chemical groups that may be determinants of the reported bioactivity of the compounds. A hit prioritization strategy using a novel “desirability scoring function” was able to identify AMNPs with the desired drug-likeness. Hit optimization strategies implemented on AMNPs with poor desirability scores led to the design of two compounds with improved desirability scores.

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Phytochemical Prospection and Modulation of Antibiotic Activity In Vitro byLippia origanoidesH.B.K. in Methicillin ResistantStaphylococcus aureus

Cited by 27 publications

References 42 publications

Antiparasitic, physiological and histological effects of the essential oil of Lippia origanoides (Verbenaceae) in native freshwater fish Colossoma macropomum

Antiparasitic, physiological and histological effects of the essential oil of Lippia origanoides (Verbenaceae) in native freshwater fish Colossoma macropomum

Antimicrobial Activity and Chemical Composition of Essential Oils from Verbenaceae Species Growing in South America

Cheminformatic Profiling and Hit Prioritization of Natural Products with Activities against Methicillin-Resistant Staphylococcus aureus (MRSA)

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