Phytochemical screening and antioxidant activity of methanol extract of Dillenia excelsa leaf

Abstract

Abstract. Lisdiani, Susanto D, Manurung H. 2022. Phytochemical screening and antioxidant activity of methanol extract of Dillenia excelsa leaf. Biodiversitas 23: 3827-3835. The aim of this study was to determine the phytochemical compounds, total flavonoid, and phenolic content, and antioxidant activity of the methanol extract of Dillenia excelsa (Jack) Martelli ex Gilg (or tanikkara) leaves. Sample D. excelsa leaves were obtained from swamp forests in Melintang Village, Muara Wis sub-district, Kutai Kartanegara district, East Kalimantan province, Indonesia. This research includes sample preparation, extraction and maceration, extract concentration using a rotary evaporator, phytochemical test, total phenolic andflavonoid content test, and antioxidant activity test using 2,2-diphenyl-1-picrylhydrazil (DPPH) and Ferric reducing antioxidant power methods (FRAP). The results of this study indicate that the methanolic extract of D. excelsa leaves contains secondary metabolites, including alkaloids, flavonoids, phenolics, triterpenoids, and tannins. The total phenolic content was 181.25 g GAE/g extract, while the total flavonoid content was 36 g QE/g extract. The antioxidant activity of the methanol extract of D. excelsa leaf DPPH method had moderate activity strength with a value of IC50is 145 ppm, while the antioxidant activity of the methanol extract of D. excelsa leaf using the FRAP method has the highest antioxidant activity value at a concentration of 100 ppm with a value of 70.833%. The result indicated that the methanolic extract D. excelsa leaves had many metabolites and potentially an antioxidant.

Effectiveness test of Diploknema oligomera extracts to the decrease in glucose levels in alloxan-induced BALB/c male mice

“…Punti contains secondary metabolites, such as phenolic compounds, flavonoids, saponins, tannins, and terpenoids (Furqan and Putri 2020). These secondary metabolites can lower blood glucose (Lisdiani et al 2022) by increasing insulin secretion (Martin and Ramos 2021) and/or regenerating pancreatic β cells (Ghorbani et al 2019). In addition, these secondary metabolites also lower blood glucose by increasing insulin sensitivity through the stimulation of insulin receptors (Xu et al 2018) on insulin target cells, such as adipose cells (Kang and Chiang 2022), liver cells, and muscle cells (Hu et al 2014).…”

“…Matusin et al (2021) examined the methanol extract of Dillenia excelsa leaves. These leaves have antihyperglycemic properties because of the triterpenoid compound effects: flavonoids, tannins, and phenolics (Lisdiani et al 2022). Meanwhile, Furqan and Putri's (2020) found that the ethanol extract of punti contains flavonoids, terpenoids, phenolics, tannins, and saponins.…”

Phytochemical, GC-MS analysis and antioxidant activities of leaf methanolic extract of Lai (Durio kutejensis), the endemic plant of Kalimantan, Indonesia

“…East Kalimantan is one of the provinces in Indonesia located in a tropical rainforest with several potential endemic florae. Some of these endemic plants from Kalimantan have been proven to have medicinal properties, including tabat barito (Ficus deltoidea), dayak onions (Eleutherine bulbosa), pasak bumi (Eurycoma longifolia), kayu kuning (Arcangelisia flava), karamunting (Rhodomyrtus tomentosa) (Sutomo et al 2013;Manurung 2018), lai (Durio kutejensis), mekai (Albertisia papuana) (Priyanti 2012;Nurbani and Sumarmiyati 2015) and tanikkara (Dillenia exelsa) (Lisdiani et al 2022). The use of plants as a source of medicine has been inherited and is an important component of the health care system.…”