Phytochemical Screening and Biological Activity of Ethanolic Extract of Rosa X Damascena Mill. Cultivated in the Western Region of Romania

Abstract

Rosa x damascena Mill. has pharmacological properties that are partially attributed to their abundance in phenolic compounds. We investigated the alcoholic extract from the plants petals. Microscopic examination of Rosa x damascena Mill. petals showed that both of the adaxial and abaxial epidermal cells contained pigments. The antimicrobial activity of the extract was determined by the disc diffusion method. The most sensitive antimicrobial effect was on the strain Pseudomonas aeruginosa. The SOD-like activity of Rosa damascena alcoholic extract has been tested both in vitro and in vivo. Three cell lines were used for determining the cytotoxicity of Rosa damascena alcoholic extract, a human cervical carcinoma line (HeLa), a human radical growth phase melanoma cell line (WM35) and a normal fibroblastic epithelial cell line (HFL1) by MTT method. The Rosa damascena extract presents SOD-like activity and is cytotoxic on HeLa and WM35 cell lines. Due to these results, Rosa x damascena extracts can be used for their bioactive potential. RezumatRosa x damascena Mill. are proprietăți farmacologice care sunt parțial atribuite abundenței lor în compuși fenolici. Am investigat extractul alcoolic din petalele acestei plante. Examinarea microscopică a petalelor a arătat că ambele celule epidermice adaxiale și abaxiale conțin pigmenți. Activitatea antimicrobiană a extractului a fost determinată prin metoda de difuzie a discului. Cel mai sensibil efect antimicrobian a fost asupra tulpinii Pseudomonas aeruginosa. Activitatea SOD a extractului alcoolic din Rosa damascena a fost testată atât in vitro, cât și in vivo. Au fost utilizate trei linii celulare pentru determinarea citotoxicității extractului alcoolic, o linie de carcinom cervical uman (HeLa), o linie celulară de melanom în fază de creștere radicală umană (WM35) și o linie celulară epitelială fibroblastică normală (HFL1) prin metoda MTT. Extractul de Rosa damascena prezintă activitate asemănătoare SOD și este citotoxic pe liniile celulare HeLa și WM35. Datorită acestor rezultate, extractele de Rosa x damascena pot fi utilizate pentru potențialul lor bioactiv.

In Vitro and Human Pilot Studies of Different Topical Formulations Containing Rosa Species for the Treatment of Psoriasis

“…Qualitative characterization of the extracts was performed by HPLC method. Identified compounds for Rosa damascena were presented and discussed in a previous article [ 17 ].…”

“…Qualitative, quantitative characterization, and antioxidant activity for the alcoholic extract of Rosa damascena can be consulted in our previous research article as phytochemical screening and biological activity shows that the extract contains is a wide range of natural sources which can be used for the discovery of natural pharmaceuticals and due to the high content of bioactive compounds (TPC = 321 ± 0.03 mg GAE/100 g DW and TFC = 32.4 ± 0.01 mg QE/100 g DW) and high values for antioxidant activity: DPPH (94.11 ± 0.01%), CUPRAC (555.316 ± 0.06 µmol Trolox/mL), FRAP (354.43 ± 0.03 µmol Trolox/mL) and ABTS (76.351 ± 0.02 µmol Trolox/mL), it can be used as a prophylactic agents or as an adjuvant treatment in the pathologic process of various disorders. [ 17 ].…”

The Effect of Extraction Method of Vernonia Amygdalina Delile. Leaves on Cardiotonic Effect

“…A standard phytochemical screening procedure was used to identify the phytochemical compounds present in the ethanolic extract of African leaves. Alkaloids were tested with Dragendorrf, Mayer and Bouchardat; flavonoids with Mg and HCl; saponins with distilled water and HCl; tannins with FeCl3; glycosides with the Molisch test; cardiac glycosides by performing the Keller-Kiliani test; and steroids and terpenoids by Liebermann's test [17][18][19][20][21].…”

“…The test solution's optical density (OD ) was measured at a wavelength of 540 nm with a UV-Vis spectrophotometer. Levels were measured by plotting the absorbance obtained with a calibration curve obtained from digoxin as standard [20].…”

Uhplc/MS Assessment of Polyphenolic Acids and Radical Scavenging Activity of Salvia Species From the South-West of Romania Flora

“…The quantification of flavonoids was made using the aluminium chloride colorimetric method when they form a yellow product, with the absorption read at 415 nm. All extracts were assessed in triplicate, with values of flavonoid contents (TFCs) expressed as mg of quercetin equivalents per gramme of dry weight (mg QE/g d.w.) calculated from the calibration curve of quercetin [12,20].…”