Phytochemical screening and cytotoxic evaluation of Bauhinia scandens leaf extracts using HeLa and T47D cell lines

Lianah, Lianah; Khasanah, Rita Ariyana Nur; Pranatami, Dwimei Ayudewandari; Krisantini, Krisantini

doi:10.13057/biodiv/d220247

Cited by 4 publications

(6 citation statements)

References 30 publications

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“…Bauhinia scandens L. belonging to the Leguminosae family is known as 'chain tree' and distinctively dispersed in Indonesia including Semarang, Central Java. Its morphologic characteristics have been described previously in our publication (Lianah, 2016;Lianah et al, 2021). In summary, Bauhinia scandens is liana with ornamental value; the stem forms into a 'monkey ladder' when older; the fruits are pea shaped with 1 to 2 seeds.…”

Section: Introductionsupporting

confidence: 53%

“…exhibited antioxidant activities (Amarowicz, 2007;Brunetti et al, 2013;Graßmann, 2005;Pietta, 2000). The previous studies, Lianah et al (2021) have reported the presence of phytochemicals with high concentration in the ethanolic extracts of Bauhinia scandens leaves, namely total phenols (17.57±0.098% w/w) and saponins (19.42±0.091% w/w), followed by tannins (1.24±0.035% w/w), alkaloids (1.31±0.001% w/w), and steroids (0.08±0.007% w/w). Nevertheless, a screening of phytochemicals in Bauhinia scandens bark has not yet been done.…”

Section: Fig 2 Linear Regression Analysis For % Inhibition Of Ethanol...mentioning

confidence: 93%

“…In summary, Bauhinia scandens is liana with ornamental value; the stem forms into a 'monkey ladder' when older; the fruits are pea shaped with 1 to 2 seeds. Apart from Jeromini et al (2020) who studied optimal growing medium for growing Bauhinia scandens seedlings and Jeromini et al (2021) who reported the methods to overcome seed dormancy, very limited studies have been conducted on propagation and culture of this species.According to previous phytochemical screening, the ethanolic extract of its leaves contained some phytochemicals such as phenols, flavonoids, saponins, tannins, alkaloids and steroids which are widely known to have strong relation with medicinal anti-oxidant properties (Lianah et al, 2021). In addition, according to preliminary cytotoxic activity evaluation against HeLa and T47D cell lines, the ethanolic extract of the Bauhinia scandens leaves had potential to be anti-cancer agent (Lianah et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

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Antioxidant Activity of Ethanolic Extract of Bauhinia scandens

Lianah¹,

Khasanah²,

Sari³

et al. 2022

EEC

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Recently, much attention has been focused on the exploration of the ethnomedicine containing the antioxidant property for limiting the harmful effects of excessive reactive oxygen species (ROS) generation in the body. Bauhinia scandens has been reported to exert antioxidant property however which part of the plant has not been fully elucidated yet. The study aimed to investigate the antioxidant activity of Bauhinia scandens especially in the bark and leaf parts. The antioxidant activity of this plant was measured using 1,1-diphenyl- 2-picrylhydrazyl (DPPH) assay method. The inhibitory concentration 50 (IC50) of each sample was calculated to show the antioxidant activity. The IC50 value of Bauhinia scandens barks and leaves ethanolic extract were 34.77 Âµg/ml and 113.50 Âµg/ml respectively and standard ascorbic acid (as positive control) IC50 was 79.57 Âµg/ml. To our knowledge, this is the first study to show Bauhinia scandens barks ethanolic extract exerts higher antioxidant activity compared to ascorbic acid. This activity may relate to the specific Phytochemicals in Bauhinia scandens.

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Section: Introductionsupporting

confidence: 53%

Section: Fig 2 Linear Regression Analysis For % Inhibition Of Ethanol...mentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

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Antioxidant Activity of Ethanolic Extract of Bauhinia scandens

Lianah¹,

Khasanah²,

Sari³

et al. 2022

EEC

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“…Indian tribal and non-tribal people of different regions use the plant pod and seeds as tonic and to overcome the problem of libido. [6] DISCUSSION: Medicinal plants play an important role in human health and have potential to treat various diseases 32 . Available scientific research on B. purpurea L. is suggested a huge biological potential of this plant leaf, flower and bark but there is very limited data available on pod (fruit) 5 .…”

Section: Flowermentioning

confidence: 99%

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Background: Bauhinia purpurea L. is a flowering plant belongs to family Leguminosae, also known as purple orchid plant. All parts of this plant including leaves, stem bark, flower and pods are edible and used for various medicinal purposes. Detailed pharmacognostic profiling of various parts has been performed except plant Pod, thus pod was selected for this study. Objective: The purpose of this work was to enrich the research on B. purpurea L. Pod in respect of pharmacognostical, physicochemical, qualitative identification of secondary metabolites by HPTLC and ethno-botanical profiling. Material and Methods: Entire pod and powder were subject to macro and microscopic study, different physicochemical parameters including ash, extractive, moisture and pH determined through standard methods with analytical grade chemical and standard equipments. Quantitative estimation of phenolic content was performed by well-known Folin-Ciocalteu method and HPTLC developed through Vision cat software. Result: Fresh pod is green, elongated with suborbicular seeds. TS show different parenchymatous arrangement with yellow brown tannin content and Ca-oxalate crystals. Alkaloids, tannin, phenolic and flavonoids were reported in the hydro-methanolic extract through phytochemical screening. Consequently, total phenol and flavonoid were found to be 490.62mg/g and 235.28 mg/g, respectively. Moreover, in HPTLC, R f values of tested extract were reported between 0.2 to 0.72. Conclusion: Reported Ethano-botanical values of different parts of this plant were denoted the range of its therapeutic importance in indifferent tribal races. B. purpurea L. is a medicinal remedy generally known as Orchid tree. Based on our research pod of this plant has phyto-constituents including alkaloids, phenols and tannins responsible for therapeutic values. Subsequently, HPTLC of hydro-methanolic extract also proved the presence of different compounds which might be support the ethano-botanical utilization of this part of plant. Hence this study would be assist for the identification, standardization of plant pod collected from various geographical area and suppliers in the authentication.INTRODUCTION: Nature provides a great gift in the form of plants to the human being to have a healthy and disease free life.

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“…The chloroform layer formed was put into a 10 mL test tube, and chloroform to 10 mL was added. The resulting solution was tested using a spectrophotometer with a wavelength of 430 nm [22]. Determination of total flavonoids.…”

Section: Phytochemical Analysismentioning

confidence: 99%

Phytochemical Analysis and Cytotoxic Activities of Hantap Leaves (<i>Sterculia coccinea</i> Jack) Extract

et al. 2023

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Hantap (Sterculia coccinea Jack) has been used traditionally for various health issues, including cancer treatment. The therapeutic effects of natural ingredients are often attributed to their chemical constituents. This study aimed to analyze the phytochemical contents and cytotoxic activities of S. coccinea leaves on HeLa and MCF-7 cancer cell lines. The quantitative phytochemical analysis was carried out following standard laboratory procedures. Phytochemical compounds were identified using LC-MS/MS QTOF. The MTT assay PrestoBlue™ Cell Viability Reagent test method was used to test cytotoxic activity in the cell culture. Extraction was carried out by the maceration method using 96% ethanol as solvent. The quantitative analysis revealed that tannins were the major phytochemical constituent in the highest percentage of 72.16%, followed by alkaloids, flavonoids, and steroids, with values of 30.80, 28.66, and 2.85%, respectively. Saponins were present in the lowest percentage of 1.15%. The ethanolic extract exhibited moderate cytotoxicity on HeLa and MCF-7 cells with IC50 values of 591.00 and 578.10 µg/mL, respectively. Identification using LC-MS/MS showed the suspected compounds 5,7-dihydroxy-3-(4'-hydroxybenzyl)chromone as homoisoflavanones and kaempferide-3-O-α-L-rhamnosyl-7-O-α-L-rhamnoside from flavonol triglycosides. These results may contribute to the study on the use of leaves extract of S. coccinea for developing a chemoprevention agent.

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Phytochemical screening and cytotoxic evaluation of Bauhinia scandens leaf extracts using HeLa and T47D cell lines

Cited by 4 publications

References 30 publications

Antioxidant Activity of Ethanolic Extract of Bauhinia scandens

Antioxidant Activity of Ethanolic Extract of Bauhinia scandens

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Phytochemical Analysis and Cytotoxic Activities of Hantap Leaves (<i>Sterculia coccinea</i> Jack) Extract

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