Phytochemical Screening and in Vitro Antioxidant Activity of Whole Plant Extracts of Sesuvium Portulacastrum L

Chintalapani, Sathvika; Swathi, M.; Narasu, M. Lakshmi

doi:10.22159/ajpcr.2018.v11i1.22558

Cited by 9 publications

(9 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Nayak et al (2018) reported presence of saponins, terpenoids, tannins, alkaloids and steroids in the methanol leaf extracts of Suaeda maritima, besides their presence in other mangroves such as Xylocarpus granatum, Avicennia marina, Salvador apersica and Avicennia officinalis (Mulla and Chavan 2016). Similar to present study, ethanolic extract of Sesuviumportula castrum possesses phytochemicals like alkaloids, carbohydrates, cardiac glycosides, flavonoids, phenols, saponins, sterols, terpenoids, quinones, diterpenes, and resins contributed to its therapeutic value (Chintalapani et al 2018).…”

Section: Discussionsupporting

confidence: 87%

Purification and characterization of bioactive compounds extracted from Suaeda maritima leaf and its impact on pathogenicity of Pseudomonas aeruginosa in Catla catla fingerlings

et al. 2021

View full text Add to dashboard Cite

Incidence of various dreadful microbial infections and the development of antibiotic resistance by infection causative microbes are the main reasons for reducing aquaculture productivity. Hence, there is an immense need for the discovery of alternative and efficient treatment for quick recovery of diseased fishes. In the present study, Suaeda maritima leaf extracts (hexane, diethyl ether, ethanol, and water) were screened for in vitro and in vivo antibacterial and antioxidant activities. Out of all the four extracts, ethanolic extract showed highest antibacterial activity against S. aureus (4.9 ± 1.3 mm), B. subtilis (1.6 ± 0.3 mm), K. pneumoniae (4.2 ± 1.8 mm), and P. aeruginosa (4.1 ± 1.2 mm). Similarly, antioxidant activity was also higher for ethanolic extract (500 µg/mL) based on DPPH radical scavenging ability (71.6 ± 1.4%) and reducing potential (149 μg/mL) assays. Further, ethanolic extract was purified consecutively via column chromatography and preparative TLC where an active fraction was selected based on highest antibacterial (10.1 ± 1.4 mm) and antioxidant properties (82.3 ± 2.8%). Active fraction was loaded onto mass spectroscopy and identified the presence of four active constituents such as 1,2,9,10-tetramethoxy-6-methyl-5,6,6a,7-tetrahydro-4H-dibenzo[de,g]quinolin-3-yl) methanol; 3',7-Dimethoxy-3-hydroxyflavone; Saponin and (19R)9acetyl19hydroxy10,14dimethyl20oxopentacyclo[11.8.0.0 < 2,10 > .0 < 4,9 > .0 < 14,19 >]henicos-17-yl-acetate. Besides, in vivo studies were conducted on Catla catla fingerlings infected with P. aeruginosa under laboratory conditions. The fingerlings were segregated into 5 groups, among which group 4 and 5 were treated with crude and purified extracts. Both the extracts were efficient in treating infected fingerlings and recorded 100% survival rate which is even better than group-3 treated with a synthetic antibiotic (77%). Hence, S. maritima leaf extract can be considered as a possible alternative medicine in aquaculture.

show abstract

Section: Discussionsupporting

confidence: 87%

Purification and characterization of bioactive compounds extracted from Suaeda maritima leaf and its impact on pathogenicity of Pseudomonas aeruginosa in Catla catla fingerlings

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Nayak et al (2018) reported presence of saponins, terpenoids, tannins, alkaloids and steroids in the methanol leaf extracts of Suaeda maritima, besides their presence in other mangroves such as Xylocarpus granatum, Avicennia marina, Salvador apersica and Avicennia o cinalis (Mulla and Chavan 2016). Similar to present study, ethanolic extract of Sesuviumportula castrum possesses phytochemicals like alkaloids, carbohydrates, cardiac glycosides, avonoids, phenols, saponins, sterols, terpenoids, quinones, diterpenes, and resins contributed to its therapeutic value (Chintalapani et al 2018).…”

Section: Discussionsupporting

confidence: 84%

Purification and Characterization of Bioactive Compounds Extracted from Suaeda Maritima Leaf and its Impact on Pathogenicity of Pseudomonas Aeruginosa in Catla Catla Fingerlings

Beulah

Divya

Sampath

et al. 2021

Preprint

View full text Add to dashboard Cite

Incidence of various dreadful microbial infections and the development of antibiotic resistance by infection causative microbes are the main reasons for reducing aquaculture productivity. Hence, there is an immense need for the discovery of alternative and efficient treatment for quick recovery of diseased fishes. In the present study, Suaeda maritima leaf extracts (hexane, diethyl ether, ethanol, and water) were screened for in vitro and in vivo antibacterial and antioxidant activities. Out of all the four extracts, ethanolic extract showed highest antibacterial activity against S.aureus (4.9±1.3 mm), B.subtilis (1.6±0.3 mm), K.pneumonia (4.2±1.8 mm), and P.aeruginosa (4.1±1.2 mm). Similarly, antioxidant activity was also higher for ethanolic extract (500 µg/ml) based on DPPH radical scavenging ability (71.6±1.4 %) and reducing potential (149 μg/mL) assays. Further, ethanolic extract was purified consecutively via column chromatography and preparative TLC where an active fraction was selected based on highest antibacterial (10.1±1.4 mm) and antioxidant properties (82.3±2.8 %). Active fraction was loaded onto mass spectroscopy and identified the presence of four active constituents such as 1,2,9,10-tetramethoxy-6-methyl-5,6,6a,7-tetrahydro-4H-dibenzo[de,g]quinolin-3-yl) methanol; 3',7-Dimethoxy-3-hydroxyflavone; Saponin and (19R)9acetyl19hydroxy10,14dimethyl20oxopentacyclo[11.8.0.0<2,10>.0<4,9>.0<14,19>]henicos-17-yl-acetate. Besides, in vivo studies were conducted on Catla catla fingerlings infected with P. aeruginosa under laboratory conditions. The fingerlings were segregated into 5 groups, among which group 4 and 5 were treated with crude and purified extracts. Both the extracts were efficient in treating infected fingerlings and recorded 100% survival rate which is even better than group-3 treated with a synthetic antibiotic (77%). Hence, S. maritima leaf extract can be considered as a possible alternative medicine in aquaculture

show abstract

“…Allow the mixture to stand for a minute. Formation of reddish-brown color in chloroform layer confirmed the presence of phytosterol [36,37].…”

Section: Salkowski Reactionmentioning

confidence: 99%

“…Folin-Ciocalteu reagent (FCR) assay was performed for the evaluation of TPC in the isolated plant extract [37,[44][45][46]. Take 150 µl of the extract and mixed in 240 µl of water and 150 µl of 0.25 N FCR.…”

Section: Determination Of Total Phenolic Content (Tpc)mentioning

confidence: 99%

Phytochemical Analysis and Evaluation of in Vitro Immunomodulatory Activity of Rhododendron Arboreum Leaves

Rawat

Bachheti

Kumar

2018

Asian J Pharm Clin Res

View full text Add to dashboard Cite

Objectives: The objectives of this study are to analyze phytoconstituents in Rhododendron arboreum leaves (qualitatively and quantitatively) and to estimate the immunomodulatory effects of extracts using different in vitro methods for intracellular and phagocytic killing potency of neutrophils, which are subsequent, involved in the process of phagocytosis by neutrophils.Methods: R. arboreum leaves extracts were separately extracted in 100% methanol, 50% methanol, and aqueous by a hot percolation technique using Soxhlet apparatus and concentrated with a vacuum rotary evaporator apparatus. The percentage yield of the extracts wares calculated. The extract was used to analyze phytoconstituents qualitatively, and total phenolic and flavonoid content were estimated. In another study, already purified methanolic extract of R. arboreum leaves, namely cytoplasmic male sterility (CMS-3) (kaempferol), RAM fr2, and transcranial magnetic stimulation (TMS-10) (ursolic acid) was used to evaluate immunomodulatory activities by nitroblue tetrazolium test, phagocytosis of killed Candida albicans.Results: Phytochemical screening of the extracts showed the presence of major classes of phytochemicals, i.e., phytosterols, flavonoids, phenols, alkaloids, carbohydrate, glycoside, sterols and steroids, terpenoids, and tannin while saponin was absent in all extract. The percent yield of leaves extract was 12.97%. Total phenolic content (TPC) expressed as gallic acid equivalents (GAE) ranged 1327.5±0.009 (in 100% methanolic extract), 1309.5±0.011 (in 50% methanolic), and 1468.5±0.006 mg GAE g−1 dry weight (in aqueous extract). Total flavonoid content expressed as rutin equivalents (RE) ranged 219±0.025 (in 100% methanolic extract), 184±0.02 (in 50% methanolic), and 262±0.58 mg (in aqueous extract) mg RE g−1 dry weight. R. arboreum purified extract CMS-3 (kaempferol), RAM fr2, and TMS-10 (ursolic acid) stimulated the neutrophils to phagocytic activity to the extent of 67.67±2.08%, 40.3±1.5%, and 79.67±0.57%, respectively. The above-said extracts stimulated the phagocytosis of killed C. albicans. The mean particle numbers for CMS- 3, RAM fr2, and TMS-10 were found to be 6.7, 6.3, and 7, respectively, at concentration 1 mg/ml.Conclusion: The phytochemical screening of all the extracts showed the presence of various phytochemicals that are biologically important. The total phenolic and flavonoid content of the plant are comparable to other medicinal plants. Even though the enormous progress on the phytochemistry and pharmacology of R. arboreum have been made, there still require more conclusive studies on the safety, efficacy, and in vivo toxicity of extracts and pure compounds to gain a better understanding.

show abstract

Phytochemical Screening and in Vitro Antioxidant Activity of Whole Plant Extracts of Sesuvium Portulacastrum L

Cited by 9 publications

References 11 publications

Purification and characterization of bioactive compounds extracted from Suaeda maritima leaf and its impact on pathogenicity of Pseudomonas aeruginosa in Catla catla fingerlings

Purification and characterization of bioactive compounds extracted from Suaeda maritima leaf and its impact on pathogenicity of Pseudomonas aeruginosa in Catla catla fingerlings

Purification and Characterization of Bioactive Compounds Extracted from Suaeda Maritima Leaf and its Impact on Pathogenicity of Pseudomonas Aeruginosa in Catla Catla Fingerlings

Phytochemical Analysis and Evaluation of in Vitro Immunomodulatory Activity of Rhododendron Arboreum Leaves

Contact Info

Product

Resources

About