Phytochemical screening and in-vitro antibacterial and DPPH free radical scavenging activities of methanol extract of root of Combretum album Pers.

Abstract

The objective of the study was to investigate in-vitro antibacterial and antioxidant activities of methanolic extract of Combretum album Pers. root. In brief antibacterial efficacies of methanol extract and its petroleum ether fraction (MePET), chloroform fraction (MeCH), ethyl acetate fraction (MeEA) and water fraction (MeAQ) were determined by agar well diffusion assay along with Minimum Inhibitory Concentrations (MICs). In-vitro antioxidant efficacies were evaluated by DPPH radical scavenging method. Preliminary phytochemical assay, Fourier Transform Infrared Spectroscopy and Gas Chromatography-Mass Spectroscopy analyses were employed to detect the plausible active ingredients. Methanol extract showed broad spectrum antibacterial activity having highest inhibition zone against Staphylococcus aureus MTCC 2940 (21.67±0.58 mm) and MICs ranged from100 µg/ml to 250 µg/ml. The scavenging activity of methanol extract of root of C. album was concentration dependant and IC50 value was 136.08 µg/ml. The lowest MIC (5 µg/ml) was noted with MeCH against B. subtilis (MTCC 441). MeCH showed highest antioxidant activity with an IC50 value of 12.98 ?g/mL and MePET, MeEA, MeAQ and Ascorbic acid presented antioxidant potential with IC50 values of 16.10, 15.07, 17.44 and 13.40??g/mL respectively. Preliminary phytochemical tests and FT-IR analysis revealed presence of various phytochemicals and functional groups like hydroxyl, carboxylic acids, amidines, amines, aromatics and esters. Three compounds were elucidated from bioactive TLC fraction of MeCH in GC-MS analysis supported by presence of various functional groups in MeCH as detected in FT-IR analysis. It is concluded that methanolic extract of C. album root is a potential antibacterial and antioxidant agent where presence of N-[5-[4-pyridinyl)-1H-1,2,4-triazol-3-yl]-benzamide (compound 1), pyruvic acid (compound 2) and methyloacetone (compound 3) are responsible as possible active ingredients.

Separation of Bio-active Compounds and in vitro Antibacterial Potency of Callistemon linearis Floral Extract with Mechanism of Action

“…7,8 Medicinal plant is a safest option in this scenario because compounds isolated from them is less harmful to human and other animals. 9 Callistemon linearis, the narrow-leaved bottlebrush is a stiff shrub upto 3-meter height having linear leaves on woody stems. In the month of spring fiery red bottlebrush flowers are bloomed out.…”

Repellent and oviposition-deterrent efficacies of Cuscuta chinensis on filarial vector Culex quinquefasciatus

“…For pest specificity, easily biodegradability, and typically lack of toxicity to higher animals [10] , efficacy, and ease of availability, mosquito control through botanicals is a very desirable area of study. Not only mosquito, plant products have multidirectional activities against helminthes [11,12] , bacteria [13,14,15] , mollusca [16] etc. Replacement of host plant may also reduce Mansonia population, vector of Brugia [17] .…”

The secretory scales and medicinal properties of combretum erythrophyllum.

“…These natural products (secondary metabolites such as phenolics, flavonoids, stilbenes, tannins and alkaloids) are known to display high levels of antioxidant activity. indicum, have been duly noted for their antioxidant activity Burman and Chandra, 2021;Mashi et al, 2021;Sharma et al, 2021;Forid et al, 2021).…”

“…The antioxidant potential of the roots of Combretum album Pers. was recently evaluated, with substantial antioxidant properties quantified from the methanolic extracts (highest antioxidant activity-IC50 12.98 μg/mL) (Burman and Chandra, 2021). Combretum leprosum Mart.…”