Phytochemical screening, free radical scavenging, antioxidant activity and phenolic content of Dodonaea viscosa

Abstract

The purpose of this study was to evaluate the antioxidant potential of Dodonaea viscosa Jacq. Methanolic extract of the plant was dissolved in distilled water and partitioned with n-hexane, chloroform, ethyl acetate and nbutanol sequentially. Phytochemical screening showed presence of phenolics, flavonoides and cardiac glycosides in large amount in chloroform, ethyl acetate and n-butanol fraction. The antioxidant potential of all these fractions and remaining aqueous fraction was evaluated by four methods: 1,1-Diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, total antioxidant activity, Ferric Reducing Antioxidant Power (FRAP) assay and ferric thiocyanate assay along with determination of their total phenolics. The results revealed that ethyl acetate soluble fraction exhibited highest percent inhibition of DPPH radical as compared to other fractions. It showed 81.14 ± 1.38% inhibition of DPPH radical at a concentration of 60 μg/ml. The IC50 of this fraction was found to be 33.95 ± 0.58 μg/ml, relative to butylated hydroxytoluene (BHT), having IC50 of 12.54 ± 0.89 μg/mL. It also showed highest FRAP value (380.53 ± 0.74 μM of trolox equivalents) as well as highest total phenolic contents (208.58 ± 1.83 GAE μg/g) and highest value of inhibition of lipid peroxidation (58.11 ± 1.49% at concentration of 500 μg/ml) as compared to the other studied fractions. The chloroform fraction showed highest total antioxidant activity i.e.1.078 ± 0.59 (eq. to BHT)

Berberis Baluchistanica: Assessment of Natural Antioxidants to Reprieve From Oxidative Stress

“…Here, ferrous chloride reacts with peroxide and produces ferric ions. The ferric ions then combine with ammonium thiocyanate and ferric thiocyanate is produced which shows reddish pigment 9 . This study revealed that polar fractions displayed good percentage inhibition of lipid peroxidation (table 3) as compared to medium and less polar fractions of this plant.…”

“…Phytochemical screening of all the five crude extracts i.e. nhexane soluble fraction, chloroform soluble fraction, ethyl acetate soluble fraction, n-butanol soluble fraction and remaining aqueous fractions was carried out to identify the phytochemical constituents (alkaloids, terpenoids, saponins, tannins, sugars, phenolics, flavonoids, cardiac glycosides) using the standard procedures [7][8][9][10] .…”

“…Several studies have shown that medicinal plants contain different nutrients and non-nutrients molecules and some molecules show antioxidant and antimicrobial activities can be useful for protection of human body against both oxidation and micro-organisms 6,7 . So in continuation of our previous studies on medicinal plants [8][9][10] , here we have screened the phytoconstituents of Berberis baluchistanica Ahrendt to explore its possible use as a natural source of antioxidants. This plant belongs to family Berberdaceae and some species of this genus e.g.…”

Strong Radical Scavenging Macrofungi from the Dry Zone Forest Reserves in Sri Lanka

“…The degree of discolouration correlates with the scavenging potential of the antioxidant compounds in the extracts in terms of hydrogen donating ability. The decrease in absorption is used as a measure of the extent of DPPH free radical scavenging activity [35,36]. Radical scavenging capacity was found to be inversely related to the EC 50 values of the investigated species [37,38].…”

Antiplasmodial Compounds from Leaves of Dodonaea angustifolia

“…Furthermore, there was no significant difference in mean body weight in all groups of mice including those groups of mice treated with the standard drug. The active fractions yielded compounds 1 (10 mg, 0.01%), 2 (40 mg, 0.04%), and 3 (30 mg, 0.03%), which were identified by comparison of the physical and spectroscopic data as the known compounds pinocembrin ( 1 ) [15], santin ( 2 ) [16] and 2 - hydroxy-15,16-epoxyceloda-3,13(16),14-trien-18-oic acid ( 3 ) [17], respectively (Fig. 3 ).…”