Phytochemical screening, FTIR and GCMS analysis of Cucurbita pepo seeds cultivated in Kiambu county, Kenya

Mwangi, John Wamumwe; Kiragu, Denis; Chaka, Bakari

doi:10.1016/j.heliyon.2024.e30237

Heliyon

2024

DOI: 10.1016/j.heliyon.2024.e30237

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Phytochemical screening, FTIR and GCMS analysis of Cucurbita pepo seeds cultivated in Kiambu county, Kenya

John Wamumwe Mwangi,

Denis Kiragu,

Bakari Chaka

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2024

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Cited by 2 publications

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Insecticidal Activity of Fatty Acids From the Fruit Extract of Momordica cymbalaria Fenzl Ex Naudin, Has Insecticidal Activities on Mosquito Larvae

Monika,

Lavanya,

Sethuraman

et al. 2024

Chemistry & Biodiversity

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The fruit and other parts of Momordica cymbalaria are known to have medicinal properties. The study investigates the chemical composition and functional groups of Momordica cymbalaria fruits to assess the insecticidal potential of its bioactive metabolites. In‐silico approach was used to understand the mechanism behind the insecticidal nature of these metabolites. Ethyl acetate extract has 18 compounds while 20 compounds were identified in methanol using GC‐MS analysis. The extracts contain several functional groups, including alkanes, esters, carboxylic acids, amides, and aromatic groups. The insecticidal bioassay using methanol and ethyl acetate extracts of Momordica cymbalaria on Aedes aegypti and Culex quinquefasciatus mosquito larvae shows the LC50 value for Aedes aegypti was 65.7 ppm and for Culex quinquefasciatus was 96.9 ppm for ethyl acetate and methanol extract is 282.36 ppm, 388.09 ppm. Molecular docking of secondary metabolites was performed with five insect proteins, namely OBP, CYP450, PPO, aaNAT and SCP. Results show that Methyl 6‐cis, 9‐cis, 11‐trans‐octadecatrienoate has high binding affinity with SCP‐2 besides showing binding with other insect proteins. The data suggest that Methyl 6‐cis, 9‐cis, 11‐trans‐octadecatrienoate has multiple insecticidal actions and may serve as a green insecticide.

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Insecticidal Activity of Fatty Acids From the Fruit Extract of Momordica cymbalaria Fenzl Ex Naudin, Has Insecticidal Activities on Mosquito Larvae

Monika,

Lavanya,

Sethuraman

et al. 2024

Chemistry & Biodiversity

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Phytoconstituents and In Vitro Free Radical Scavenging Potential of n-Hexane and Aqueous Fractions of Cucurbita maxima and Leptadenia hastata

Dahiru,

Danga,

Oni

et al. 2024

sciphar

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The present study explored the phytoconstituents and radical scavenging activity of the respective n-hexane and aqueous fractions of Cucurbita maxima (CMHF and CMAF) and Leptadenia hastata (LHHF and LHAF) for potential application in oxidative stress-related ailments. The phytoconstituents were qualitatively determined and characterized using Fourier-transform Infrared (FTIR), while the antioxidant activity was determined in vitro. Alkaloids were present in only the aqueous fractions of C. maxima and L. hastata, while saponins, steroids, and flavonoids were detected in all the fractions. The FTIR revealed the presence of functional groups, including alcohols, sulfonates, alkenes, alkanes, amines, and aromatics in both plant fractions. The LHHF (35.53 ±2.11 ascorbic acid (AA) equivalent µg/mL) exhibited a significantly (p<0.05) higher total reducing power (TRP) than all the other fractions. The CMHF (69.11 ±2.56 AAE µg/mL) demonstrated a significantly (p<0.05) higher total antioxidant capacity (TAC) than all the other fractions. For the ferric thiocyanate (FTC) assay, the highest inhibition was exhibited by LHHF (79.78 ± 3.24%), significantly (p<0.05) higher than AA (26.46 ± 2.12%), CMHF (69.77 ± 3.16%), and CMAF (43.80 ± 2.12%). In the thiobarbituric acid assay, the lowest MDA concentration was exhibited by the CMHF (0.07 ±0.01 nmol/mL), significantly (p<0.05) lower than all the other fractions and ascorbic acid. Conclusively, the n-hexane fraction of both plants presents potential sources of novel antioxidant compounds with significant free radical scavenging and anti-lipid peroxidation activities, applicable in ailments linked to oxidative stress.

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Phytochemical screening, FTIR and GCMS analysis of Cucurbita pepo seeds cultivated in Kiambu county, Kenya

Cited by 2 publications

References 38 publications

Insecticidal Activity of Fatty Acids From the Fruit Extract of Momordica cymbalaria Fenzl Ex Naudin, Has Insecticidal Activities on Mosquito Larvae

Insecticidal Activity of Fatty Acids From the Fruit Extract of Momordica cymbalaria Fenzl Ex Naudin, Has Insecticidal Activities on Mosquito Larvae

Phytoconstituents and In Vitro Free Radical Scavenging Potential of n-Hexane and Aqueous Fractions of Cucurbita maxima and Leptadenia hastata

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