Phytochemical Screening, Total Flavonoid and Total Phenolic Content and Antioxidant Activity of Different Parts of Caesalpinia bonduc (L.) Roxb

Sembiring, Elin Novia; Elya, Berna; Sauriasari, Rani

doi:10.5530/pj.2018.1.22

Cited by 180 publications

(147 citation statements)

References 15 publications

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“…The ethanol was chosen in this study because its considered safer, and environmentally friendly compared to other solvents. Moreover, several studies have demonstrated higher antioxidant activity as well as total phenolic content when extracted with ethanol in Caesalpinia bonduc, 19 seagrass T. testidunum, 10 palm kernel-by product 14 and in black tea and mate tea. 20 Another significant influence in obtaining the phenolic content and antioxidant activity is the proportion of water in ethanol.…”

Section: Discussionmentioning

confidence: 99%

Optimization of Extraction Condition to Obtain Antioxidant Activity and Total Phenolic Content of Seagrass Thalassia hemprichii (Ehrenb.) Asch from Indonesia

Nopi¹,

Anwar²,

Nurhayati³

2018

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Background: Thalassia hemprichii (Ehrenb.) Asch is one of the commonly found seagrasses spread over the coast of Indonesia and has not been utilized. Previous studies have reported its potency as an antioxidant, however, studies on optimal extraction conditions using ethanol as a solvent to obtain higher antioxidant activity are still limited. Objective: The purpose of this study is to optimize the extraction condition for obtaining the highest antioxidant activity and total phenolic content of the seagrass T. hemprichii. In an addition, percentage yield of extract was also evaluated. Methods: In this study, factorial design with independent factors of ethanol concentrations (50%, 70%, and 100%), the use of HCl 1 N (Yes/No), and temperature (30 0 C, 60 0 C) was developed. The analysis of variance (ANOVA) was used to determine the significance of the above factors towards antioxidant activity and total phenolic content. The antioxidant activity was evaluated with 2,2-diphenyl-1-picrylhydrazyl (DPPH) method and the total phenolic content was determined by Folin-Ciocalteu method. Re sults: The highest antioxidant activity (IC 50 =83.48 μg/mL) was shown in sample R1 (extracted with 50% ethanol, added with HCl 1 N, and 60 0 C). The sample was also showed total phenolic content 41.03 mg GAE per gram and 22% yield extract. Among the factors examined, the used of HCl 1 N showed the most significant effect to the antioxidant activity and the total phenolic content, with the p-value of 0.048 and 0.034 respectively (p<0.05). Conclusion: This extraction condition can be applied to obtain higher antioxidant activity and total phenolic content from the plant.

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Section: Discussionmentioning

confidence: 99%

Optimization of Extraction Condition to Obtain Antioxidant Activity and Total Phenolic Content of Seagrass Thalassia hemprichii (Ehrenb.) Asch from Indonesia

Nopi¹,

Anwar²,

Nurhayati³

2018

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“…Total flavonoid content was measured using the aluminum chloride colorimetric assay, adapted from a previous protocol. 20 Aqueous FD and SD extracts at 20-500 mg/mL and the product or placebo product or different dilutions of standard quercetin solution (4.7-75.0 mg/ mL) (Aldrich, Germany) were added in 100 µL of 10% AlCl 3 (BDH PROLABO, Germany). Then, 100 µL of sodium acetate (1.0 mol/L) (Ajax Finechem Pty Ltd, Germany) was added to 2.8 mL of deionized water.…”

Section: Total Flavonoid Contentmentioning

confidence: 99%

Potential of Hard Candy Containing Spray-Dried Vernonia cinerea Extract with Total Phenolic Compounds, Total Flavonoids and Nicotine Replacement as an Anti-Smoking Aid

Kanthain¹,

Singhatong²,

Natakankitkul³

et al. 2020

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Backgound: Vernonia cinerea (VC) is a natural plant claimed to reduce cigarette smoking. Some pilot anti-smoking products with nicotine replacement, such as lozenges or gum, have been presented, but with some adverse effects. Thus, application of VC as a new-anti-smoking product is very challenging. Objectives: The aims of this study were to compare the active compounds; total phenolic compounds, total flavonoids and nicotine, and study antioxidant activity on scavenging 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and 1,1-diphenyl-2-picryl hydrzayl (DPPH) radicals of extracts prepared by spray drying (SD) and freeze drying (FD) techniques for pilot hard candy. Methods: Raw VC materials of mixed parts, i.e., the stem, flowers and leaves, were made to form extracts by FD and SD techniques. Then, extract from the SD technique was manufactured industrially into hard candy containing glucose syrup and refined glucose. Total phenolic compounds, total flavonoids, nicotine, scavenging activity of extracts, VC hard candy and placebo candy were evaluated by folin-ciocalteau reagent, aluminum chloride colorimetric assay, high-performance liquid chromatography, ABTS cation decolorization and DPPH protocols. Results: Total phenolic compounds were significantly different between extracts, but total flavonoids and nicotine were slightly higher in SD extract. Antioxidant activity of both extracts on ABTS radicals was not significantly different, but the half-maximal inhibitory concentration (IC50) on DPPH radicals was significantly higher in SD extract when compared to the FD extract. Finally, total phenolic compounds, total flavonoids and nicotine, as well as scavenging activity could be detected in hard candy. Conclusion: VC can be used as an anti-smoking aid with nicotine replacement and anti-oxidant compounds in pilot hard candy.

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“…All determinations were carried out in triplicates. The total phenolic content was expressed as the number of equivalents of gallic acid (GAE) [29,31].…”

Section: Total Phenolic Contentmentioning

confidence: 99%

“…Blank was methanol. The absorption of the standard quercetin solution (20-100 μg/mL) in methanol was measured under the same conditions [29,31].…”

Section: Total Flavonoid Contentmentioning

confidence: 99%

Evaluation of possible antioxidant activities of barberry solid formulation, a selected formulation from Traditional Persian Medicine (TPM) via various procedures

2019

Biointerface Res Appl Chem

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The purpose of this study is to reformulate a solid traditional Ghors-e- zereshk (barberry traditional tablet) from Traditional Persian Medicine (TPM) and to assess the antioxidant effect and determining the total phenol and flavonoids contents of water and hydroalcoholic extract of mentioed formulation. The antioxidant effects of water and hydroalcoholic extracts of barberry solid formulation were measured by three methods: DPPH radical scavenging, nitric oxide free radical scavenging and Ferric Reducing Antioxidant Power Assay (FRAP) reducing method. The total phenol and flavonoid content were measured by Folin-Ciocalteu and aluminum chloride colorimetric methods. For standardization ashes’ test also used. The hydro-alcoholic extract has more phenolic compounds (31.46±.13 mg GAE∕g of dry matter) than the water extract and the water extract has more flavonoid compounds (6.38±. 25 mg Quercetin ∕g of dry matter) than the hydro-alcoholic extract. The result of DPPH radical scavenging shows that the hydro-alcoholic extract has more effect than the water extract. FRAP value indicates that the hydro-alcoholic extract has more reductive activity than the water extract. The study of NO radical scavenging represents water extract 74.61±2.95 %, hydro-alcoholic extract 61.72±1.79 % thus, water and hydro-alcoholic extracts are powerful antioxidants and have hepatoprotective effects. Other relative biological effects, for example, the protective effects through oxidative stress can also be considered.

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Phytochemical Screening, Total Flavonoid and Total Phenolic Content and Antioxidant Activity of Different Parts of Caesalpinia bonduc (L.) Roxb

Cited by 180 publications

References 15 publications

Optimization of Extraction Condition to Obtain Antioxidant Activity and Total Phenolic Content of Seagrass Thalassia hemprichii (Ehrenb.) Asch from Indonesia

Optimization of Extraction Condition to Obtain Antioxidant Activity and Total Phenolic Content of Seagrass Thalassia hemprichii (Ehrenb.) Asch from Indonesia

Potential of Hard Candy Containing Spray-Dried Vernonia cinerea Extract with Total Phenolic Compounds, Total Flavonoids and Nicotine Replacement as an Anti-Smoking Aid

Evaluation of possible antioxidant activities of barberry solid formulation, a selected formulation from Traditional Persian Medicine (TPM) via various procedures

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