Phytochemical Screening, Total Phenolic and Flavonoids Contents and Antioxidant Activities of Citrullus Colocynthis L. And Cannabis Sativa L.

Abstract

Citrullus colocynthis L. and Cannabis sativa L. are well known herbs for their curative value and farming applications. Thus, extraction was performed to examine yield, total phenolic, flavonoids content and antioxidant activities for farming exertions. Qualitative analyses were conducted to scrutinize alkaloids, glycosides, terpenoids, flavonoids, flavones, steroids, tannins, phenols, saponins and quantitative analysis for total phenolic and flavonoids content was assessed by Folin-Ciocalteu and aluminium chloride colourimetric method respectively, while antioxidant activities was assessed by using 1, 1-Diphenyl-1-picrylhydrazyl. Results showed that distilled water afforded the maximum extract yield followed by methanol and ethanol thus, coefficient of determination (R 2 ) of extract yield present a positive correlation compared to root mean square error (RMSE). Phytoconstituents except terpenoids from C. sativa and C. colocynthis leaves were reported while, in contrast, steroids, tannins and phenols were absent in C. colocynthis roots. The methanol derived maximum phenolic contents from C. sativa and C. colocynthis leaves were 36.42 and 37.69 mg gallic acid equivalent GAE/g respectively. However, total flavonoids registered from C. sativa leaves and C. colocynthis leaves and roots were 59.03, 50.58 and 43.32 mg quercetin equivalent QE/g respectively. Interestingly, C. colocynthis leaves produced the highest flavonoids 119.63 mg QE/g using ethyl acetate extract. DPPH inhibition (%) was high in acetone 55.57, hexane 45.98 and distilled water 35.5% from C. sativa, C. colocynthis leaves and roots respectively. Our findings suggest that studied plants contain phytochemicals, reasonable quantity of phenol and flavonoids content confer to the potential antioxidant activity responsible for insecticidal properties as safer alternatives of synthetic pesticides.

Insecticidal activity and biochemical composition of Citrullus colocynthis, Cannabis indica and Artemisia argyi extracts against cabbage aphid (Brevicoryne brassicae L.)

“…Previously, it was reported that ethanol yielded more than other solvents from leaves of Melastoma malabathricum 34 . Furthermore, a relatively high extract yield from C. colocynthis and C. sativa using ethanol as an extraction solvent was reported 35 .…”

Mycochemical Screening and Analysis, Antioxidant Activity, and Biochemical Composition of Fermentation Strain Snef1216 (Penicillium chrysogenum)

“…Presence of phenols was determined according to Ahmed et al [21]. 3 ml distilled water and 3-4 drops of 10% ferric chloride (FeCl 3 ) were poured into 1.5 ml of the fermentation solution.…”

Phytochemical screening, GC-MS profiling of invasive cocklebur (Xanthium strumarium)-insect-pathogen interaction and simulated volatile chemical signaling at Northeast China

“…After complete evaporation the sample was weighed and first yield was calculated; however the same procedure was adopted for thrice to make sure that the whole polar and non-polar materials were eluted finally [114] stored at 4°C in air tight sterile tubes [115]. Physical properties of each extract (color, stickiness and appearance) recorded visually (Table 1) [116].…”

“…Phytochemical analysis were assessed according to the standards protocol to find out presence of various chemical compounds such as alkaloids, saponins, terpenoids and steroids [117], glycosides, flavonoids and flavones [118,119] phenols and tannins using different solvents [115,116,120]…”