PHYTOCHEMICAL SCREENING, TOTAL PHENOLIC AND TOTAL FLAVONOID CONTENT, AND ANTIOXIDANT ACTIVITY OF DIFFERENT PARTS OF Melastoma malabathricum

Danladi, Suleiman; Wan-Azemin, Amirah; Sani, Yahaya Najib; Mohd, Khamsah Suryati; Rao, U.S. Mahadeva; Mansor, Sharif Mahsufi; Dharmaraj, Saravanan

doi:10.11113/jt.v77.5988

Cited by 13 publications

(10 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It is one of the most abundant and diversified groups of pantropical families, having 4200 to 4500 species in 166 genera and approximately 1000 species predominantly cultivated in tropical Asia [4]. In Malaysia, particularly, the genus Melastoma consists of 22 species, 2 subspecies, and 3 varieties, which can be distinguished by the color of their flower petals, such as Melastoma malabathricum (dark-purple magenta), Melastoma decemfidum (white), and Melastoma hirta [4][5][6][7]. Studies have reported that members of this family have been used by practitioners of traditional medicines to treat diarrhea, pox scars, inflamed wounds, gastric ulcers, and diabetes [8][9][10].…”

Section: Introductionmentioning

confidence: 99%

“…Studies have reported that members of this family have been used by practitioners of traditional medicines to treat diarrhea, pox scars, inflamed wounds, gastric ulcers, and diabetes [8][9][10]. The phytochemistry of these plants has revealed numerous secondary metabolites or bioactive compounds, such as flavonoids and phenolic compounds [5]. Moreover, previous reports have shown the isolation of several flavonoids from M. decemfidum yields naringenin, kaempferol, kaempferol-3-O-D-glucoside, and kaempferol-3-O-[2 6 -di-O-p-trans-coumaroyl], which are responsible for their antioxidant activities [11].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Optimization of the Antioxidant Activities of Mixtures of Melastomataceae Leaves Species (M. malabathricum Linn Smith, M. decemfidum, and M. hirta) Using a Simplex Centroid Design and Their Anti-Collagenase and Elastase Properties

et al. 2020

View full text Add to dashboard Cite

In this study, a simplex-centroid mixture design (SCMD) approach was used to select the optimal proportions of three different Melastomataceae leaves species (M. malabathricum, M. decemfidum, and M. hirta) extracts to determine the optimum antioxidant activities of total phenolic compound (TPC) and ABTS (2, 2′-azino-bis (ethylbenzthiazoline-6-sulfonic acid)) radical-scavenging activities. Twelve experimental designs were set up, consisting of points that were equally weighted mixtures of 0 to 1 components, which were pure blends (1, 0, …, 0), binary blends (1/2, 1/2, 0, …, 0), tertiary blends (1/3, 1/3, 1/3, 0, …, 0), and a control point. The in vitro anti-elastase and collagenase activities were evaluated in order to determine the anti-aging efficacy of the optimized mixture extracts. A high-performance liquid chromatography (HPLC) analysis was employed to identify the flavonoid content (rutin and quercetin) present in the optimized mixture extracts. The results showed that the best proportions of the optimum phenolic compounds and ABTS activity corresponded to 30%, 40%, and 30% of M. malabathricum, M. hirta, and M. decemfidum leaf extracts, respectively. The in vitro anti-collagenase and elastase activity evaluation of the optimized mixture extracts showed 70% inhibition against both elastase and collagenase enzymes. The HPLC analysis revealed two flavonoids (rutin and quercetin) at retention time 7.770 and 8.769 min, respectively, in the mixture extracts. This study suggests the potential exploitation of mixtures of Melastomataceae leaves (M. malabathricum, M. decemfidum, and M. hirta) as cosmetic ingredients for antioxidant and anti-wrinkles applications.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Optimization of the Antioxidant Activities of Mixtures of Melastomataceae Leaves Species (M. malabathricum Linn Smith, M. decemfidum, and M. hirta) Using a Simplex Centroid Design and Their Anti-Collagenase and Elastase Properties

et al. 2020

View full text Add to dashboard Cite

show abstract

“…A B Figure 1. Color of Senggani fruit extract with drying treatment, A: sun-drying treatment; B: oven drying treatment Phytochemical testing was utilized to determine quickly and simply whether a plant contains certain bioctive compounds or not (Danladi, et al, 2015). This test was performed employing chemical reactions.…”

Section: Resultsmentioning

confidence: 99%

The Effect of Simplisia Drying Method on Antioxidant Activity of Senggani Fruit Extract (Melastoma Malabathricum L.) by DPPH (2,2-diphenyl-1-picrylhydrazyl)

2021

View full text Add to dashboard Cite

Senggani fruit (Melastoma Malabathricum L.) contains anthocyanin that functioning as an antioxidant. Anthocyanin are tremendously sensitive to thermal processes which trigger phytochemical or photo-oxidation reactions that can open anthocyanin rings. The objective of this study is to identify the effect of the simplicia drying method on the antioxidant activity of Senggani fruit extract. Senggani fruit extract was prepared by obtaining samples of ripe fruit, dry sorting, washing, wet sorting, and drying using two methods; sunlight and oven at 70oC. After the simplicia was dry, it was blended and sifted until smooth. The fine simplicia was macerated with 96% ethanol and evaporated to gain a crude extract. The crude extract was assessed with reagents for phytochemical screening. Furthermore, the crude extract was examined for antioxidant activity by the DPPH method. This study implies that the simplicia and crude extract of Senggani fruit from drying in sunlight and oven possess different organoleptic properties such as color, smell, and taste. In phytochemical testing with reagents, it was discovered that anthocyanin compounds were unveiled in drying utilizing sunlight while employing an oven at 70oC; no anthocyanins were found. The antioxidant testing of Senggani fruit extract revealed that the drying method employing sunlight had an IC50 value of 18.8 g/mL while the oven temperature of 70oC owned an IC50 value of 28.3 g/mL. Based on the study results, it can be identified that the simplicia drying method affects the antioxidant activity of the Senggani fruit extract. The drying method in the sun produces extracts with greater antioxidant activity while drying in an oven at 70oC results in a degradation process of anthocyanin compounds, thereby decreasing the antioxidant activity of the Senggani fruit extract.

show abstract

“…The qualitative screening of Melastomataceae leaf extracts has been widely reported [66][67][68]; however, to date, no available studies have reported on the biochemical properties of various parts of M. malabathricum L supplemented with FW compost and PK biochar. The results revealed the presence of flavonoids and phenols (Table 2).…”

Section: Discussionmentioning

confidence: 99%

Organic Amendments Effects on Nutrient Uptake, Secondary Metabolites, and Antioxidant Properties of Melastoma malabathricum L.

Rusli

Abdullah

Yaacob

et al. 2022

Plants

View full text Add to dashboard Cite

Amelioration of soil acidity can boost soil fertility, hence increasing nutrient uptake, secondary metabolite, and its antioxidant potential. In the present study, the effectiveness of food waste compost and palm kernel biochar was assessed as soil amendments for Melastoma malabathricum L. grown in acidic soil conditions. A six-month greenhouse study was conducted using completely randomized design (CRD) with three treatment groups, including control plants (T1), plants amended with palm kernel biochar (T2), and plants amended with food waste compost (T3). Data analysis revealed that Melastoma malabathricum L. amended with T3 recorded the highest total chlorophyll content (433.678 ± 13.224 µg g−1 DW), followed by T2 and T1. The increase in chlorophyll content was contributed by the increase in soil pH. This was shown by the positive significant correlations between soil pH and chlorophyll a (r2 = 0.96; p ≤ 0.01) and chlorophyll b (r2 = 0.778; p ≤ 0.01). In addition, the same treatment exhibited the highest total anthocyanin content (leaves; 36.1 × 10−2 ± 0.034 mg/g DW and root extract; 8.9 × 10−2 ± 0.020 mg/g DW), total phenolic content (stem extract; 4930.956 ± 16.025 mg GAE/g DE), and total flavonoid content (stem extract; 209.984 ± 0.572 mg QE/g DE). Moreover, this study also found that the highest antioxidant potential against 2,2-Diphenyl-1-picrylhydrazyl (DPPH) and 2,2-Azinobis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radicals was exhibited by samples supplemented with food waste compost (T3), followed by palm kernel biochar (T2). This indicates that the soil amendments have the capacity to enhance the secondary metabolites that protect plants, therefore ameliorating Melastoma malabathricum L.’s response towards acidic stress, and resulting in better antioxidant properties. Furthermore, this study also recorded better nutrient uptake in T3. With the significantly higher levels of macronutrient in the soil, the food waste compost could enhance the nutrient properties, secondary metabolites, and antioxidant capacity of Melastoma malabathricum L. grown in acidic soil conditions.

show abstract

PHYTOCHEMICAL SCREENING, TOTAL PHENOLIC AND TOTAL FLAVONOID CONTENT, AND ANTIOXIDANT ACTIVITY OF DIFFERENT PARTS OF Melastoma malabathricum

Cited by 13 publications

References 17 publications

Optimization of the Antioxidant Activities of Mixtures of Melastomataceae Leaves Species (M. malabathricum Linn Smith, M. decemfidum, and M. hirta) Using a Simplex Centroid Design and Their Anti-Collagenase and Elastase Properties

Optimization of the Antioxidant Activities of Mixtures of Melastomataceae Leaves Species (M. malabathricum Linn Smith, M. decemfidum, and M. hirta) Using a Simplex Centroid Design and Their Anti-Collagenase and Elastase Properties

The Effect of Simplisia Drying Method on Antioxidant Activity of Senggani Fruit Extract (Melastoma Malabathricum L.) by DPPH (2,2-diphenyl-1-picrylhydrazyl)

Organic Amendments Effects on Nutrient Uptake, Secondary Metabolites, and Antioxidant Properties of Melastoma malabathricum L.

Contact Info

Product

Resources

About