Phytochemical study and evaluation of cytotoxicity, mutagenicity, cell cycle kinetics and gene expression of Bauhinia holophylla (Bong.) Steud. in HepG2 cells in vitro

Ribeiro, Diego Luís; Cilião, Heloísa Lizotti; Specian, Ana Flávia Leal; Serpeloni, Juliana Mara; Oliveira, Marcelo Tempesta de; Varanda, Eliana Aparecida; Vilegas, Wagner; Saldanha, Luiz Leonardo; Martínez‐López, Wilner; Dokkedal, Anne Lígia; Cólus, I.M.S.

doi:10.1007/s10616-017-0173-5

Cited by 9 publications

(7 citation statements)

References 46 publications

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“…The anti-inflammatory activities were evaluated as the decrease in the production of the pro-inflammatory cytokines TNF-α and interleukin-6 (IL-6) and the increase of the level of the anti-inflammatory cytokine IL-1 [ 97 ]. Anti-ulcer activity has also been described in models of acute gastric lesion induced in rats or mice, and aqueous extracts promoted an increase in the amount of gastric mucus [ 99 ]. The potential gastroprotective activity is possibly mediated by flavonols.…”

Section: Biological Activitymentioning

confidence: 99%

“Cow’s Hoof” (Bauhinia L., Leguminosae): A Review on Pharmacological Properties of Austral South American Species

Fortunato

Nores

2022

Plants

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The genus Bauhinia s.l. (Leguminosae), known as cow’s hoof, unha de boi or pata de vaca, has been used in traditional medicine worldwide. The aim of the present review is to summarize the studies published on the biological activity of the main native medicinal species reported in austral South America. Of the 14 species present in the region, 10 are consumed as leaf infusions to regulate glucose and lipid metabolism, as well as used for their anti-inflammatory and analgesic effects and to treat various diseases. Pharmacological properties have been recorded in seven species. Antioxidant, anticoagulant, antihypertensive, diuretic, antimicrobial and antitumor properties have been reported in B. forficata. Together with B. holophylla, they are important for their antidiabetic properties, since several studies indicate their effectiveness as a hypoglycemic agent. B. bauhinioides is distinguished for its anti-inflammatory and antithrombotic activities and S. microstachya for its analgesic properties. Anti-ulcer and wound healing activities recorded in B. holophylla and B. ungulata, respectively, are of particular interest. Most of the species possess antitumor activity. The antioxidant capacity of flavonoids and other bioactive compounds make these plants good candidates to assist or treat various alterations related with oxidative stress, such as diabetic complications. Thus, these species constitute promising targets for new bioactive substance research and phytotherapy.

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Section: Biological Activitymentioning

confidence: 99%

“Cow’s Hoof” (Bauhinia L., Leguminosae): A Review on Pharmacological Properties of Austral South American Species

Fortunato

Nores

2022

Plants

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“…Flavonoids from B. holophylla showed a significant antiviral effect against the Dengue virus, which also belongs to the Flaviviridae family [14]. Ribeiro et al [15] and Marena et al [16] reported the absence of cytotoxic and mutagenic effects, as well as antimicrobial effects of hydroalcoholic leaf extract, suggesting that B. holophylla has potential as an herbal medicine.…”

Section: Introductionmentioning

confidence: 99%

Antiviral Activity of Flavonoids from Bauhinia holophylla Leaves against Zika virus

Thomasi,

Teixeira,

Lopes

et al. 2024

Microbiology Research

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Zika virus (ZIKV) is involved in the etiology of serious nervous system pathologies. Currently, there are no specific and effective vaccines or antiviral drugs to prevent the diseases caused by ZIKV. This study aimed to assess the activity of flavonoids present in crude hydroethanolic extract (CHE) and fractions obtained from B. holophylla leaves against ZIKV. O-glycosylated flavonoids were characterized by high-performance liquid chromatography coupled with high-resolution mass spectrometry (LC-HRMS/MS). The cytotoxic concentration and the effective concentration for 50% of the cells (CC50 and EC50, respectively) were determined, and the selectivity index (SI) was calculated. Molecular networks were constructed based on the chemical composition of the samples and global antiviral activity data using the Global Natural Products Social Molecular Networking (GNPS) platform. Protein–ligand docking was performed in the NS2B-NS3 protease, NS3 helicase, and NS5 methyltransferase of the ZIKV. CHE showed greater antiviral activity at a multiplicity of infection (MOI) of 1.0, with an EC50 of 11.93 µg/mL, SI = 13.38, and reduced cytopathic effects. Molecular networks indicated that O-glycosylated flavonoids are responsible for the activity against ZIKV, being quercetin-O-deoxyhexoside more selective and effective. Molecular docking confirmed the inhibitory activity of quercetin-O-deoxyhexoside, which showed an affinity for the tested targets, especially for NS2B-NS3 protease. The results showed that B. holophylla has flavonoids with potential for future therapeutic applications against ZIKV.

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“…Antiviral effects were also described by Santos et al [ 28 ] against the dengue virus. Recently, Ribeiro et al [ 29 ] and Marena et al [ 30 ] reported the absence of cytotoxic and mutagenic effects, and even protective activity against known carcinogens, as well as antimicrobial effects of hydroalcoholic leaf extract, suggesting that B. holophylla has potential as a herbal medicine.…”

Section: Introductionmentioning

confidence: 99%

Flavonoid-Rich Fractions of Bauhinia holophylla Leaves Inhibit Candida albicans Biofilm Formation and Hyphae Growth

Fonseca

Teixeira

Ferreira

et al. 2022

Plants

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This study evaluated the effect of the extract and fractions of Bauhinia holophylla on Candida albicans planktonic growth, biofilm formation, mature biofilm, and hyphae growth. Three C. albicans strains (SC5314, ATCC 18804, and ATCC 10231) were tested. The crude extract and the fractions were obtained by exhaustive percolation and liquid–liquid partition, respectively. Phytochemical analyses of B. holophylla extract and fractions were performed using high-performance liquid chromatography coupled with a diode-array detector and mass spectrometry (HPLC-DAD-MS). A microdilution assay was used to evaluate the effect of the B. holophylla extract and fractions on C. albicans planktonic growth, and crystal violet staining was used to measure the total biomass of the biofilm. Hyphae growth was analyzed using light microscopy. Thirteen flavonoids were identified, with a predominance of the flavonol-3-O-glycoside type based on quercetin, myricetin, and kaempferol. Flavonoid-rich fractions of B. holophylla leaves displayed antifungal activity and inhibited both biofilm formation and hyphae growth in all the tested strains, but were not effective on C. albicans planktonic growth and mature biofilm. This study indicates that flavonoid-rich fractions from B. holophylla leaves interfere with the virulence of Candida species and support the use of Bauhinia spp. in folk medicine to treat infections.

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Phytochemical study and evaluation of cytotoxicity, mutagenicity, cell cycle kinetics and gene expression of Bauhinia holophylla (Bong.) Steud. in HepG2 cells in vitro

Cited by 9 publications

References 46 publications

“Cow’s Hoof” (Bauhinia L., Leguminosae): A Review on Pharmacological Properties of Austral South American Species

“Cow’s Hoof” (Bauhinia L., Leguminosae): A Review on Pharmacological Properties of Austral South American Species

Antiviral Activity of Flavonoids from Bauhinia holophylla Leaves against Zika virus

Flavonoid-Rich Fractions of Bauhinia holophylla Leaves Inhibit Candida albicans Biofilm Formation and Hyphae Growth

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