Phytochemicals: A Promising Alternative for the Prevention of Alzheimer’s Disease

Koul, Bhupendra; Farooq, Usma; Yadav, Dhananjay; Song, Mi Soon

doi:10.3390/life13040999

Cited by 18 publications

(1 citation statement)

References 246 publications

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“…They also have several synergistic benefits, such as improving cognitive and cholinergic functioning. A great amount of scientific research has demonstrated the anti-Alzheimer's disease benefits of polyphenols, including cell damage protection, neuroprotective effects, AChE inhibitory action, and amyloid aggregation [62,63].…”

Section: Phytochemical Estimation Of Khudari Fruit Pulpmentioning

confidence: 99%

Antioxidant, LC-MS Analysis, and Cholinesterase Inhibitory Potentials of Phoenix dactylifera Cultivar Khudari: An In Vitro Enzyme Kinetics and In Silico Study

Almalki,

Alqurashi,

Alturaiki

et al. 2023

Biomolecules

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We evaluated the therapeutic potentials of Khudari fruit pulp, a functional food and cultivar of Phoenix dactylifera, against neurological disorders. Our results demonstrate a good amount of phytochemicals (total phenolic content: 17.77 ± 8.21 µg GA/mg extract) with a high antioxidant potential of aqueous extract (DPPH assay IC50 = 235.84 ± 11.65 µg/mL) and FRAP value: 331.81 ± 4.56 µmol. Furthermore, the aqueous extract showed the marked inhibition of cell-free acetylcholinesterase (electric eel) with an IC50 value of 48.25 ± 2.04 µg/mL, and an enzyme inhibition kinetics study revealed that it exhibits mixed inhibition. Thereafter, we listed the 18 best-matched phytochemical compounds present in aqueous extract through LC/MS analysis. The computational study revealed that five out of eighteen predicted compounds can cross the BBB and exert considerable aqueous solubility. where 2-{5-[(1E)-3-methylbuta-1,3-dien-1-yl]-1H-indol-3-yl}ethanol (MDIE) indicates an acceptable LD50. value. A molecular docking study exhibited that the compounds occupied the key residues of acetylcholinesterase with ΔG range between −6.91 and −9.49 kcal/mol, where MDIE has ∆G: −8.67 kcal/mol, which was better than that of tacrine, ∆G: −8.25 kcal/mol. Molecular dynamics analyses of 100 ns supported the stability of the protein–ligand complexes analyzed through RMSD, RMSF, Rg, and SASA parameters. TRP_84 and GLY_442 are the most critical hydrophobic contacts for the complex, although GLU_199 is important for H-bonds. Prime/MM-GBSA showed that the protein–ligand complex formed a stable confirmation. These findings suggest that the aqueous extract of Khudari fruit pulp has significant antioxidant and acetylcholinesterase inhibition potentials, and its compound, MDIE, forms stably with confirmation with the target protein, though this fruit of Khudari dates can be a better functional food for the treatment of Alzheimer’s disease. Further investigations are needed to fully understand the therapeutic role of this plant-based compound via in vivo study.

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Section: Phytochemical Estimation Of Khudari Fruit Pulpmentioning

confidence: 99%

Antioxidant, LC-MS Analysis, and Cholinesterase Inhibitory Potentials of Phoenix dactylifera Cultivar Khudari: An In Vitro Enzyme Kinetics and In Silico Study

Almalki,

Alqurashi,

Alturaiki

et al. 2023

Biomolecules

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Enzyme Inhibitory Activities and RP‐HPLC Analysis of Geranium and Erodium Species

Özüpek,

Abaci Kaplan,

Nazlı Gok

et al. 2024

Chemistry & Biodiversity

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The genera Geranium and Erodium (Geraniaceae) have been documented to possess diverse ethnopharmacological uses, including diabetes mellitus. Relevant to their ethnopharmacological use, the current study aimed to evaluate the α‐glucosidase, α‐amylase, acetylcholinesterase (AChE), and butyrylcholinesterase (BChE) enzyme inhibitory activity of ethanol extracts from 46 samples belonging to thirty‐one species of Geranium (20) and Erodium (11) collected throughout Türkiye. The majority of the extracts displayed a marked α‐glucosidase and α‐amylase inhibitory activity. Besides, 23 extracts out of 46 exhibited a concentration‐dependent inhibitory effect over 50% towards AChE. The highest AChE inhibition was found in G. subcaulescens collected from Konya with an IC50 value of 4.73 ± 2.96 µg/mL. E. somanum, E. leucanthum, and E. sipthorpianum exhibited the most potent α‐glucosidase inhibitory activity, while E. birandianum and E. pelargoniiflorum were the most active extracts against AChE and BChE, respectively. Three extracts that had inhibitory activity over 50% against four of the enzymes were selected and proceeded to RP‐HPLC analysis. Geraniin and ellagic acid were identified as major compounds in the active extracts. Most of the species screened in the current study were examined for the first time against α‐glucosidase, α‐amylase, AChE, and BChE.

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Updates on Global Status of Transgenic and Genome-Edited Crops

Koul,

Pudhuvai,

Bhanot

et al. 2024

Genetic Engineering of Crop Plants for Food and Health Security

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Phytochemicals: A Promising Alternative for the Prevention of Alzheimer’s Disease

Cited by 18 publications

References 246 publications

Antioxidant, LC-MS Analysis, and Cholinesterase Inhibitory Potentials of Phoenix dactylifera Cultivar Khudari: An In Vitro Enzyme Kinetics and In Silico Study

Antioxidant, LC-MS Analysis, and Cholinesterase Inhibitory Potentials of Phoenix dactylifera Cultivar Khudari: An In Vitro Enzyme Kinetics and In Silico Study

Enzyme Inhibitory Activities and RP‐HPLC Analysis of Geranium and Erodium Species

Updates on Global Status of Transgenic and Genome-Edited Crops

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