Phytochemicals: Extraction, Isolation, and Identification of Bioactive Compounds from Plant Extracts

Abstract

There are concerns about using synthetic phenolic antioxidants such as butylated hydroxytoluene (BHT) and butylated hydroxyanisole (BHA) as food additives because of the reported negative effects on human health. Thus, a replacement of these synthetics by antioxidant extractions from various foods has been proposed. More than 8000 different phenolic compounds have been characterized; fruits and vegetables are the prime sources of natural antioxidants. In order to extract, measure, and identify bioactive compounds from a wide variety of fruits and vegetables, researchers use multiple techniques and methods. This review includes a brief description of a wide range of different assays. The antioxidant, antimicrobial, and anticancer properties of phenolic natural products from fruits and vegetables are also discussed.

RETRACTED: Dose‐dependent chemopreventive effects of citronellol in DMBA‐induced breast cancer among rats

“…Many phytochemicals possess anti-cancer properties that is, potential to negate the production of reactive oxygen species (ROS). The antioxidant potential of phytochemicals is considered beneficial in cancer prevention through scavenging of free radicals (Altemimi, Lakhssassi, Baharlouei, Watson, & Lightfoot, 2017).…”

Analysis of alkaloids (indole alkaloids, isoquinoline alkaloids, tropane alkaloids)

“…C-NMR is used to identify the types of carbon are present in the compound, whereas H-NMR is used for searching the hydrogen nature of any compound. 15.5.16 Mass spectrometry (Altemimi et al, 2017;Kumar, 2017;Rammohan et al, 2016) Mass spectrometry (MS) is the most popular and powerful technique for qualitative as well as quantitative analysis of indole, isoquinoline, and tropane derivatives. The application of this technique is structure elucidation as well as identify the physical and chemical characteristics of indole, isoquinoline, and tropane derivatives.…”

Phytochemical evaluation and GC-MS analysis of <i>Hyptis verticillata</i> cultivated in Calabar Cross River State, Nigeria

“…The relative percentage of each phytocomponent was calculated by comparing its average peak area to the total area. The name, molecular weight and structure of the components of the test materials were ascertained in the absence of pure standards (Uraku, 2016, Altemimi et al, 2017.…”