Phytochrome B interacts with SWC6 and ARP6 to regulate H2A.Z deposition and photomorphogensis in <i>Arabidopsis</i>

Wei, Xuxu; Wang, Wanting; Xu, Peng; Wang, Wenxiu; Guo, Tongtong; Kou, Shuang; Liu, Minqing; Niu, Yake; Yang, Huey‐Lang; Mao, Zhilei

doi:10.1111/jipb.13111

Cited by 28 publications

(14 citation statements)

References 90 publications

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“…Interestingly, chromatin-remodeling mutants are also hyposensitive to red and far-red light, indicating that the SWR1 complex is also important for phytochrome signaling. Further studies have shown that phytochrome B interacts with SWC6 and ARP6 ( Mao et al, 2021 ; Wei et al, 2021 ).…”

Section: Signal Transduction Through Cryptochrome-interacting Proteinsmentioning

confidence: 99%

Signaling Mechanisms by Arabidopsis Cryptochromes

Ponnu

Hoecker

2022

Front. Plant Sci.

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Cryptochromes (CRYs) are blue light photoreceptors that regulate growth, development, and metabolism in plants. In Arabidopsis thaliana (Arabidopsis), CRY1 and CRY2 possess partially redundant and overlapping functions. Upon exposure to blue light, the monomeric inactive CRYs undergo phosphorylation and oligomerization, which are crucial to CRY function. Both the N- and C-terminal domains of CRYs participate in light-induced interaction with multiple signaling proteins. These include the COP1/SPA E3 ubiquitin ligase, several transcription factors, hormone signaling intermediates and proteins involved in chromatin-remodeling and RNA N6 adenosine methylation. In this review, we discuss the mechanisms of Arabidopsis CRY signaling in photomorphogenesis and the recent breakthroughs in Arabidopsis CRY research.

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Section: Signal Transduction Through Cryptochrome-interacting Proteinsmentioning

confidence: 99%

Signaling Mechanisms by Arabidopsis Cryptochromes

Ponnu

Hoecker

2022

Front. Plant Sci.

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“…After the imbibed seeds were keep at 4°C for 3 days, they were grown on Murashige-Skoog (MS) nutrient medium plus 2% sucrose with 0.8% agar at 22°C under white light. The experiments involving red and far-red light illuminations were described previously ( Wei et al, 2021 ). Light spectra and intensity were measured with a Hand Held spectroradiometer (ASD) and a Li250 quantum photometer (Li-Cor) ( Wei et al, 2021 ).…”

Section: Methodsmentioning

confidence: 99%

“…The experiments involving red and far-red light illuminations were described previously ( Wei et al, 2021 ). Light spectra and intensity were measured with a Hand Held spectroradiometer (ASD) and a Li250 quantum photometer (Li-Cor) ( Wei et al, 2021 ).…”

Section: Methodsmentioning

confidence: 99%

“…Pull-down assays were performed as described previously with minor modifications ( Wang et al, 2018 ). pCold-TF-phyA-N (His-TF-phyA-N), pCold-TF-phyA-C (His-TF-phyA-C), pCold-TF-phyB-N (His-TF-phyB-N), and pCold-TF-phyB-C (His-TF-phyB-C) constructs were made previously ( Wei et al, 2021 ). The DNA fragment of BIN2 was cloned into pGEX-4T-1 (GE Healthcare Life Sciences, United States).…”

Section: Methodsmentioning

confidence: 99%

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Phytochromes A and B Mediate Light Stabilization of BIN2 to Regulate Brassinosteroid Signaling and Photomorphogenesis in Arabidopsis

et al. 2022

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Phytochromes A and B (phyA and phyB) are the far-red and red lights photoreceptors mediating many light responses in Arabidopsis thaliana. Brassinosteroid (BR) is a pivotal phytohormone regulating a variety of plant developmental processes including photomorphogenesis. It is known that phyB interacts with BES1 to inhibit its DNA-binding activity and repress BR signaling. Here, we show that far-red and red lights modulate BR signaling through phyA and phyB regulation of the stability of BIN2, a glycogen synthase kinase 3 (GSK3)-like kinase that phosphorylates BES1/BZR1 to inhibit BR signaling. The BIN2 gain-of-function mutant bin2-1 displays an enhanced photomorphogenic phenotype in both far-red and red lights. phyA-enhanced accumulation of BIN2 promotes the phosphorylation of BES1 in far-red light. BIN2 acts genetically downstream from PHYA to regulate photomorphogenesis under far-red light. Both phyA and phyB interact directly with BIN2, which may promote the interaction of BIN2 with BES1 and induce the phosphorylation of BES1. Our results suggest that far-red and red lights inhibit BR signaling through phyA and phyB stabilization of BIN2 and promotion of BES1 phosphorylation, which defines a new layer of the regulatory mechanism that allows plants to coordinate light and BR signaling pathways to optimize photomorphogenesis.

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“…ACT2 was used as an internal control for quantitative reverse transcription PCR (qRT‐PCR). qRT‐PCR analysis was described previously (Wei et al ., 2021). The primers used are listed in Supporting Information Table S1.…”

Section: Methodsmentioning

confidence: 99%

Arabidopsis cryptochrome 1 undergoes COP1 and LRBs‐dependent degradation in response to high blue light

et al. 2021

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Arabidopsis cryptochrome 1 (CRY1) is an important blue light photoreceptor that promotes photomorphogenesis under blue light. The blue light photoreceptors CRY2 and phototropin 1, and the red/far-red light photoreceptors phytochromes B and A undergo degradation in response to blue and red light, respectively. This study investigated whether and how CRY1 might undergo degradation in response to high-intensity blue light (HBL).We demonstrated that CRY1 is ubiquitinated and degraded through the 26S proteasome pathway in response to HBL. We found that the E3 ubiquitin ligase constitutive photomorphogenic 1 (COP1) is involved in mediating HBL-induced ubiquitination and degradation of CRY1. We also found that the E3 ubiquitin ligases LRBs physically interact with CRY1 and are also involved in mediating CRY1 ubiquitination and degradation in response to HBL.We further demonstrated that blue-light inhibitor of cryptochromes 1 interacts with CRY1 in a blue-light-dependent manner to inhibit CRY1 dimerization/oligomerization, leading to the repression of HBL-induced degradation of CRY1.Our findings indicate that the regulation of CRY1 stability in HBL is coordinated by COP1 and LRBs, which provides a mechanism by which CRY1 attenuates its own signaling and optimizes photomorphogenesis under HBL.

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Phytochrome B interacts with SWC6 and ARP6 to regulate H2A.Z deposition and photomorphogensis in Arabidopsis

Cited by 28 publications

References 90 publications

Signaling Mechanisms by Arabidopsis Cryptochromes

Signaling Mechanisms by Arabidopsis Cryptochromes

Phytochromes A and B Mediate Light Stabilization of BIN2 to Regulate Brassinosteroid Signaling and Photomorphogenesis in Arabidopsis

Arabidopsis cryptochrome 1 undergoes COP1 and LRBs‐dependent degradation in response to high blue light

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