Phytogenic feed additive (PFA) standardized in labdane diterpens have a protective effect in Salmo salar against Piscirickettsia salmonis

Romero, Alex; Pérez, Tatiana; Santibáñez, Natacha; Vega, Matías; Miranda, Paula de Almeida Barbosa

doi:10.1016/j.aquaculture.2020.736170

Cited by 5 publications

(5 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results are similar to those previously reported for this compound in rainbow trout [32]. On the other hand, the same effects have been observed in other nutritional supplements for salmonids based on natural products [35,36].…”

Section: Discussionsupporting

confidence: 92%

See 1 more Smart Citation

[Cu(NN1)2]ClO4, a Copper (I) Complex as an Antimicrobial Agent for the Treatment of Piscirickettsiosis in Atlantic Salmon

Parra,

Aldabaldetrecu,

Arce

et al. 2024

IJMS

View full text Add to dashboard Cite

Piscirickettsia salmonis is the pathogen that most affects the salmon industry in Chile. Large quantities of antibiotics have been used to control it. In search of alternatives, we have developed [Cu(NN1)2]ClO4 where NN1 = 6-((quinolin-2-ylmethylene)amino)-2H-chromen-2-one. The antibacterial capacity of [Cu(NN1)2]ClO4 was determined. Subsequently, the effect of the administration of [Cu(NN1)2]ClO4 on the growth of S. salar, modulation of the immune system and the intestinal microbiota was studied. Finally, the ability to protect against a challenge with P. salmonis was evaluated. The results obtained showed that the compound has an MIC between 15 and 33.9 μg/mL in four isolates. On the other hand, the compound did not affect the growth of the fish; however, an increase in the transcript levels of IFN-γ, IL-12, IL-1β, CD4, lysozyme and perforin was observed in fish treated with 40 μg/g of fish. Furthermore, modulation of the intestinal microbiota was observed, increasing the genera of beneficial bacteria such as Lactobacillus and Bacillus as well as potential pathogens such as Vibrio and Piscirickettsia. Finally, the treatment increased survival in fish challenged with P. salmonis by more than 60%. These results demonstrate that the compound is capable of protecting fish against P. salmonis, probably by modulating the immune system and the composition of the intestinal microbiota.

show abstract

Section: Discussionsupporting

confidence: 92%

“…The immunomodulatory effect of the compound could be the mechanism of action through which the compound is capable of generating protection against P. salmonis. Similar mechanisms of action were reported for treatments based on natural compounds against P. salmonis [35,36].…”

Section: Discussionsupporting

confidence: 72%

[Cu(NN1)2]ClO4, a Copper (I) Complex as an Antimicrobial Agent for the Treatment of Piscirickettsiosis in Atlantic Salmon

Parra,

Aldabaldetrecu,

Arce

et al. 2024

IJMS

View full text Add to dashboard Cite

show abstract

“…Interestingly, the recent identification of the QS molecule N‐acetyl‐homoserine lactone (C2‐HSL) produced by P. salmonis (Ruiz et al., 2021) could represent a potential target of action for the Andrographolide‐based PFA. In addition, PFA has shown a protective effect on SHK‐1 cells infected with P. salmonis , associated with an increase of il‐12 and ifn‐I mRNAs and reduced bacterial cytotoxicity (Romero et al., 2021). Thus, the bacteriostatic properties of PFA could improve the cellular immune response and positively regulate key immune gene expression either directly or indirectly (Zhang et al., 2020).…”

Section: Discussionmentioning

confidence: 99%

“…Andrographolide is the major labdane diterpenoid isolated from A. paniculate , which has been reported to exhibit antibacterial (Majumdar et al., 2020; Mary & Banu, 2017; Singha et al., 2003), anti‐inflammatory, and anticancer activities both in vitro and in vivo (Dai et al., 2019; Zhang et al., 2020). Our previous work reported the protective effect of PFA on SHK‐1 Salmo salar macrophage cells and fish infected with P. salmonis (Romero et al., 2021), but the direct effect of PFA on the bacteria was not evaluated. Therefore, the aim of this study was to assess the possible antibacterial effect of a Phytogenic Fed Additive (PFA) extract from A. paniculata on both planktonic growth and the biofilm formation of two P. salmonis strains.…”

Section: Introductionmentioning

confidence: 99%

In vitro effects of phytogenic feed additive on Piscirickettsia salmonis growth and biofilm formation

Santibáñez,

Vega,

Pérez

et al. 2024

Journal of Fish Diseases

Self Cite

View full text Add to dashboard Cite

Piscirickettsiosis is the main cause of mortality in salmonids of commercial importance in Chile, which is caused by Piscirickettsia salmonis, a Gram‐negative, γ‐proteobacteria that can produce biofilm as one of its virulence factors. The Chilean salmon industry uses large amounts of antibiotics to control piscirickettsiosis outbreaks, which has raised concern about its environmental impact and the potential to induce antibiotic resistance. Thus, the use of phytogenic feed additives (PFA) with antibacterial activity emerges as an interesting alternative to antimicrobials. Our study describes the antimicrobial action of an Andrographis paniculate‐extracted PFA on P. salmonis planktonic growth and biofilm formation. We observed complete inhibition of planktonic and biofilm growth with 500 and 400 μg/mL of PFA for P. salmonis LF‐89 and EM‐90‐like strains, respectively. Furthermore, 500 μg/mL of PFA was bactericidal for both evaluated bacterial strains. Sub‐inhibitory doses of PFA increase the transcript levels of stress (groEL), biofilm (pslD), and efflux pump (acrB) genes for both P. salmonis strains in planktonic and sessile conditions. In conclusion, our results demonstrate the antibacterial effect of PFA against P. salmonis in vitro, highlighting the potential of PFA as an alternative to control Piscirickettsiosis.

show abstract

“…Since SBM inclusion information from this diet was unavailable, samples of fish before the trial (pre-feeding trial, PF), were analyzed for any abnormal clinical signs to ensure intestinal health. Additionally, fish were screened using qPCR standard procedures to ensure they were pathogen-free, including Piscirickettsia salmonis, Piscine orthoreovirus (PRV) and infectious pancreatic necrosis virus (IPNV), as described in (Romero et al, 2021) (Supplementary Table 2). After that, the fish were randomly distributed into four treatment groups in duplicate (80 fish in each group, 40 fish per tank), and fed with the diets for 28 days as described in Urań et al (2008).…”

Section: Experimental Design and Sampling Proceduresmentioning

confidence: 99%

Aloe vera reduces gut inflammation induced by soybean meal in Atlantic salmon (Salmo salar)

et al. 2022

View full text Add to dashboard Cite

Plant-based protein sources, such as soybean, are widely used in fish nutrition due to their market availability, wide distribution and acceptable nutritional quality. However, in some fish species, soybean meal-based diets cause gut inflammation, decreasing both nutrient absorption and growth rates. A suitable alternative to avoid these problems could be the application of additives with anti-inflammatory activity to the diet. In this study, an Aloe vera (Aloe barbadensis Miller, AV) extract was analyzed as a dietary additive to reduce the gut inflammation in Atlantic salmon (Salmo salar) fed with soybean meal (SBM) diet. Fish were distributed in four duplicated groups and fed 28 days with fish meal control diet (FM), AV inclusion diet (AV), FM diet supplemented with AV (FM+AV), SBM diet to induce enteritis and SBM+AV. The fish gut response to these treatments was analyzed in distal intestine by histopathological scores, tissue morphometric measurements and immune gene expression parameters. The score results in fish fed with SBM-based diet clearly showed enteritis, meanwhile fish fed with AV supplemented diet significantly reduced the intestinal SBM signs of damage. These findings were associated to reduction of goblet cells number, lamina propria thickness and sub-epithelial mucosa size, with a significant decrease on pro-inflammatory cytokine il-1β to basal levels, similar to those present in fish fed FM diets. In conclusion, the administration of AV in salmon diet showed a protective intestinal activity against the detrimental effects of SBM, opening the possibility to improve its use as a feed additive in aquafeeds.

show abstract

Phytogenic feed additive (PFA) standardized in labdane diterpens have a protective effect in Salmo salar against Piscirickettsia salmonis

Cited by 5 publications

References 46 publications

[Cu(NN1)2]ClO4, a Copper (I) Complex as an Antimicrobial Agent for the Treatment of Piscirickettsiosis in Atlantic Salmon

[Cu(NN1)2]ClO4, a Copper (I) Complex as an Antimicrobial Agent for the Treatment of Piscirickettsiosis in Atlantic Salmon

In vitro effects of phytogenic feed additive on Piscirickettsia salmonis growth and biofilm formation

Aloe vera reduces gut inflammation induced by soybean meal in Atlantic salmon (Salmo salar)

Contact Info

Product

Resources

About