Phytoplasmas associated with grapevine yellows disease in Iran: first report of a ‘<i>Candidatus</i> Phytoplasma trifolii’‐related strain and further finding of a ‘<i>Ca</i>. P. solani’‐related strain

Shahryari, Fatemeh; Allahverdipour, Tohid; Rabiei, Zahra

doi:10.5197/j.2044-0588.2019.040.017

Cited by 4 publications

(3 citation statements)

References 5 publications

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“…"Ca. P. trifolii" is also prevalent pathogen for various hosts in Asia, Europe and, American continents, including tomato in Jordan (Anfoka et al, 2003), pepper in Mexico (Reveles-Torres et al, 2018), grapevine, tomato, soybean, safflower in Iran (Shahryari et al, 2019;Ghayeb Zamharir and Aldaghi, 2018;Salehi et al, 2008;Davoodi et al, 2019), cabbage, sesame, periwinkle, eggplant, pepper in Turkey (Oksal et al, 2017;Ulubaş Serçe and Yılmaz, 2019;Özdemir, 2017;Sertkaya et al, 2007), in American Elm (Ulmus americana L.) in U.S.A (Flower et al, 2018), willow in China (Zhang et al, 2012), grapevine in Italy (Zambon et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Stolbur and Clover Proliferation Phytoplasma Infections in Tomato from Bingöl province, Turkey

Güller¹,

Usta²

2020

Türk Tarım Ve Doğa Bilimleri Dergisi

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Phytoplasma-type symptoms were noted in tomato (Lycopersicum esculentum L.) in Bingöl province of Turkey. The remarkable symptoms include witch's broom, rosetting, purple curled and crispy leaves, small leaves in the upper branches and excessively elongated calyx. Genomic DNA isolation was performed to identify possible pathogens from the leaves of 11 plants with and without symptom. In the Nested-PCR test performed using universal primer sets, DNA bands of approximately 1200 bp size were obtained in 4 of 11 samples. Randomly chosen two DNA bands primed R16F2n/R16R2 were cloned into an appropriate plasmid vector to further characterizations. The recombinant plasmid DNAs purified were sequenced in both directions. Molecular assays of the 16S rRNA sequence confirmed the existence of the "Canditatus Phytoplasma solani" (16SrXII-A group) (similarity coefficient 1.00) (Accession no: MT279680) and the "Canditatus Phytoplasma trifolii" (16SrVI-A group) (similarity coefficient 1.00) (Accession no: MT279852) in the infected tomato samples. The isolates associated with tomato-phytoplasma were named as 'Bingöl D11' and 'Bingöl D90' isolates, respectively. The phylogenetic dendrogram created also confirmed where both pathogens belong. This current paper is documented in the first record of "Ca. P. solani" (16SrXII-A) and "Ca. P. trifolii" (16SrVI-A) in naturally diseased tomato in Bingöl of Turkey.

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Section: Discussionmentioning

confidence: 99%

Stolbur and Clover Proliferation Phytoplasma Infections in Tomato from Bingöl province, Turkey

Güller¹,

Usta²

2020

Türk Tarım Ve Doğa Bilimleri Dergisi

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“…P. trifolii' has extensively been reported from diverse hosts such as weeds, agricultural and industrial crops including tomato, pepper, grapevine, soybean, safflower, cabbage, maize, sesame, periwinkle, eggplant, American Elm (Ulmus americana), Norfolk Island pine (Araucaria heterophylla), willow, rapeseed (Brassica napus), Suaeda aegyptiaca, Erigeron canadensis, and Sorghum halepense. Current literature screens have shown that infective pathogen has a wider distribution in Asian, European and American countries such as primarily Turkey and Iran, followed by Jordan, Mexico, USA, China, India, and Italy (Reveles-Torres et al, 2018;Shahryari et al, 2019;Ghayeb Zamharir and Aldaghi, 2018;Salehi et al, 2008;Davoodi et al, 2019;Oksal et al, 2017;Ulubaş Serçe and Yılmaz, 2019;Özdemir, 2017;Sertkaya et al, 2007;Flower et al, 2018;Zhang et al, 2012;Zambon et al, 2018;Seyahooei et al, 2017;Amiri Mazraie et al, 2018;Gupta et al, 2010;.…”

Section: Discussionmentioning

confidence: 99%

Detection and Molecular Characterization of ‘Candidatus Phytoplasma Trifolii’, a Member of the Clover Proliferation Grup, Infecting Tomato Plants from Iğdır Province in Turkey

Usta

Güller

2020

Turkish JAF Sci.Tech.

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The tomato plant exhibiting leaf rolling, witches’ broom, distorted and elongated ﬂower’s sepals in Iğdır province, Turkey, was observed. Total DNA extraction was performed from the symtomatic fresh tomato sample. All DNAs were subjected to Direct and Nested polymerase chain reaction (PCR) with universal primer sets that amplified the 16S rRNA of phytoplasmas. PCR products were purified from agarose gel and cloned into the pGEM T-Easy cloning vector. Recombinant plasmids were introduced into the prokaryotic cloning bacteria by electroporation. Plasmid isolation was performed by selecting one of the positive clones randomly and sequencing was performed by Next Generation Sequencing (NGS). Sequencing results revealed that the 16S rRNA gene associated with phytoplasma was 1251 nucleotides in size, and this sequence was denominated as ‘Iğdır 10’ isolates and recorded in the GenBank under the MT344968 accession number. The virtual restriction fragment length polymorphism (V-RFLP) and phylogenetic analysis of the 16S rDNA sequence confirmed that the cause of disease in infected tomato plants was 'Candidatus Phytoplasma trifolii' ('Ca. P. trifolii') (16SrVI-A, Clover proliferation group), with a 1.00 similarity coefficient. This present study is the first report of ‘Ca. P. trifolii’ and its nucleotide sequence analysis in naturally infected tomato in Iğdır province.

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“…To the best of our knowledge, this is the first report of 16SrV phytoplasmas in Georgia and in the Caucasus region. Considering the neighboring countries, 16SrV phytoplasmas were previously found in association with GY in Turkey and Iran (Ertunc et al 2015;Shahryari et al 2019). It is reasonable to hypothesize that, in Georgia, such 16SrV phytoplasmas could be the ones associated with flavescence dorée.…”

Section: Detection Of Grapevine Yellows (Gy) Phytoplasmasmentioning

confidence: 92%

Journal of Plant Protection Research

Megrelishvili,

Khidesheli,

Elbakidze

et al. 2022

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During field surveys conducted from July to October 2018-2020 in the eastern part of Georgia (Caucasus region), 145 out of 8000 (1.8%) and 147 out of 6600 (2.2%) grapevine plants, respectively, from mother stock/collection fields and commercial vineyards, were found exhibiting typical or suspicious grapevine yellows (GY) symptoms. Most of the symptomatic grapevine plants of Georgian cultivars showed mild symptoms with no berry alterations. Leaf samples from symptomatic plants were analyzed by serological (DAS--ELISA) and molecular (two previously published protocols of TaqMan triplex real-time PCR, here named Set I and Set II) tools for detecting GY-associated phytoplasmas. The presence of GY phytoplasmas was not detected in any examined grapevines by a serological method. GY phytoplasmas were identified in 22.41% and 6.9% symptomatic plants from mother stock and collection vineyards and in 48.3 and 19.0% symptomatic plants from commercial vineyards by Set I and Set II PCRs, respectively. As expected from previous studies reporting the wide presence of bois noir (BN) in Georgian vineyards, 'Candidatus Phytoplasma solani'(CaPsol) was detected in most phytoplasma-infected plants (47.6%), with the highest infection rate in Chardonnay. Phytoplasmas belonging to taxonomic group 16SrV were detected in 45.6% of the phytoplasma-infected grapevines. To the best of our knowledge, this is the first report of 16SrV phytoplasmas in Georgia and in the Caucasus region. Further molecular typing of 16SrV phytoplasma strains is necessary to determine if such strains are associated with flavescence dorée (FD). The knowledge of typical GY symptoms and the utilization of accurate diagnostic tools are crucial for preventing pathogen spread and producing healthy planting material. Based on the results obtained in this study, the presence of BN and 16SrV phytoplasmas should be monitored in the next years using triplex real-time PCR.

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Phytoplasmas associated with grapevine yellows disease in Iran: first report of a ‘Candidatus Phytoplasma trifolii’‐related strain and further finding of a ‘Ca. P. solani’‐related strain

Cited by 4 publications

References 5 publications

Stolbur and Clover Proliferation Phytoplasma Infections in Tomato from Bingöl province, Turkey

Stolbur and Clover Proliferation Phytoplasma Infections in Tomato from Bingöl province, Turkey

Detection and Molecular Characterization of ‘Candidatus Phytoplasma Trifolii’, a Member of the Clover Proliferation Grup, Infecting Tomato Plants from Iğdır Province in Turkey

Journal of Plant Protection Research

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