Phytotoxic effect and molecular mechanism induced by graphene towards alfalfa (Medicago sativa L.) by integrating transcriptomic and metabolomics analysis

Zhao, Chen; Guo, Zhipeng; Niu, Junpeng; Xu, Nan; Sui, Xin; Kareem, Hafiz Abdul; Hassan, Mahmood Ul; Yan, Mingke; Zhang, Quan; Wang, Zhaolan; Mi, Fugui; Cui, Jian; Wang, Quanzhen

doi:10.1016/j.chemosphere.2021.133368

Cited by 15 publications

(4 citation statements)

References 66 publications

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“…This is in accordance with some of the previous reports where graphene was shown to affect photosynthesis in a dose‐dependent manner by reducing chlorophyll biosynthesis (Hu, Lu, et al, 2014 ; Wang et al, 2019 ; Xiao et al, 2022 ; Zhang et al, 2016 ; Zhao et al, 2022 ). Graphene‐based materials are known to cause damage to the structure of chloroplasts and upregulate metabolites such as glyconic acid and aconitic acid that negatively affects chlorophyll biosynthesis (Chen, Guo, et al, 2022 ; Hu, Lu, et al, 2014 ; Zhang et al, 2016 ). Further analysis must be carried out for confirming the possible mechanisms by which higher graphene concentrations can affect chlorophyll biosynthesis in soybean.…”

Section: Discussionmentioning

confidence: 99%

Effect of graphene on soybean root colonization by Bradyrhizobium strains

Sethu Madhavan,

Montanez Hernandez,

et al. 2023

Plant Direct

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Legume crops such as soybean obtain a large portion of their nitrogen nutrition through symbiotic nitrogen fixation by diazotrophic rhizobia bacteria in root nodules. However, nodule occupancy by low‐capacity nitrogen‐fixing rhizobia can lead to lower‐than‐optimal levels of nitrogen fixation. Seed/root coating with engineered materials such as graphene‐carrying biomolecules that may promote specific attraction/attachment of desirable bacterial strains is a potential strategy that can help overcome this rhizobia competition problem. As a first step towards this goal, we assessed the impact of graphene on soybean and Bradyrhizobium using a set of growth, biochemical, and physiological assays. Three different concentrations of graphene were tested for toxicity in soybean (50, 250, and 1,000 mg/l) and Bradyrhizobia (25, 50, and 100 mg/l). Higher graphene concentrations (250 mg/l and 1,000 mg/l) promoted seed germination but slightly delayed plant development. Spectrometric and microscopy assays for hydrogen peroxide and superoxide anion suggested that specific concentrations of graphene led to higher levels of reactive oxygen species in the roots. In agreement, these roots also showed higher activities of antioxidant enzymes, catalase, and ascorbate peroxidase. Conversely, no toxic effects were detected on Bradyrhizobia treated with graphene, and neither did they have higher levels of reactive oxygen species. Graphene treatments at 250 mg/l and 1,000 mg/l significantly reduced the number of nodules, but rhizobia infection and the overall nitrogenase activity were not affected. Our results show that graphene can be used as a potential vehicle for seed/root treatment.

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Section: Discussionmentioning

confidence: 99%

Effect of graphene on soybean root colonization by Bradyrhizobium strains

Sethu Madhavan,

Montanez Hernandez,

et al. 2023

Plant Direct

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“…Disruption to photosynthesis and chlorophyll bleaching by rohitukine exposure in A. thaliana plants could be the reason for lesser concentrations of sugars in treated samples. There are several studies that have shown the negative impacts of extracts and pure compounds isolated from different plant species on recipient plants in the form of decreased levels of carbohydrates [ 92 , 93 , 94 ]. The concentration of myo-inositol was found to be slightly decreased upon rohitukine exposure.…”

Section: Discussionmentioning

confidence: 99%

Exogenously Applied Rohitukine Inhibits Photosynthetic Processes, Growth and Induces Antioxidant Defense System in Arabidopsis thaliana

et al. 2022

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The secondary metabolite rohitukine has been reported in only a few plant species, including Schumanniophyton magnificum, S. problematicum, Amoora rohituka, Dysoxylum acutangulum and D. gotadhora. It has several biological activities, such as anticancer, anti-inflammatory, antiadipogenic, immunomodulatory, gastroprotective, anti-implantation, antidyslipidemic, anti-arthritic and anti-fertility properties. However, the ecological and physiological roles of rohitukine in parent plants have yet to be explored. Here for the first time, we tried to decipher the physiological effect of rohitukine isolated from D. gotadhora on the model system Arabidopsis thaliana. Application of 0.25 mM and 0.5 mM rohitukine concentrations moderately affected the growth of A. thaliana, whereas a remarkable decrease in growth and the alteration of various morphological, physiological and biochemical mechanisms were observed in plants that received 1.0 mM of rohitukine as compared to the untreated control. A. thaliana showed considerable dose-dependent decreases in leaf area, fresh weight and dry weight when sprayed with 0.25 mM, 0.5 mM and 1.0 mM of rohitukine. Rohitukine exposure resulted in the disruption of photosynthesis, photosystem II (PSII) activity and degradation of chlorophyll content in A. thaliana. It also triggered oxidative stress in visualized tissues through antioxidant enzyme activity and the expression levels of key genes involved in the antioxidant system, such as superoxide dismutase (SOD), peroxidase (POD) and ascorbate peroxidase (APX). Rohitukine-induced changes in levels of metabolites (amino acids, sugars, organic acids, etc.) were also assessed. In light of these results, we discuss (i) the likely ecological importance of rohitukine in parent plants as well as (ii) the comparison of responses to rohitukine treatment in plants and mammals.

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“…The extended feeding time of the pine caterpillar weakened the flow of limonene to naringenin, which under the catalysis of flavanone 3-hydroxylase (F3H) resulted in the formation of dihydroflavonols, such as dihydroquercetin, dihydromyricetin, and dihydrokaempferol [49]. In alfalfa, graphene can induce the upregulated expression of dihydroquercetin and proline to participate in ROS clearance in the plant and protect it from oxidative damage [50]. These results are consistent with the changes in metabolites in sugarcane after Mythimna separate (Walker) feeding and in tea trees following caterpillar feeding [51,52].…”

Section: Flavonoid Biosynthesis Involved In Plant-induced Resistance ...mentioning

confidence: 99%

The Key Role of Plant Hormone Signaling Transduction and Flavonoid Biosynthesis Pathways in the Response of Chinese Pine (Pinus tabuliformis) to Feeding Stimulation by Pine Caterpillar (Dendrolimus tabulaeformis)

Zhao,

Sun,

Liu

et al. 2024

IJMS

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In nature, plants have developed a series of resistance mechanisms to face various external stresses. As understanding of the molecular mechanisms underlying plant resistance continues to deepen, exploring endogenous resistance in plants has become a hot topic in this field. Despite the multitude of studies on plant-induced resistance, how plants respond to stress under natural conditions remains relatively unclear. To address this gap, we investigated Chinese pine (Pinus tabuliformis) using pine caterpillar (Dendrolimus tabulaeformis) under natural conditions. Healthy Chinese pine trees, approximately 10 years old, were selected for studying induced resistance in Huangtuliangzi Forestry, Pingquan City, Chengde City, Hebei Province, China. Pine needles were collected at 2 h and 8 h after feeding stimulation (FS) via 10 pine caterpillars and leaf clipping control (LCC), to simulate mechanical damage caused by insect chewing for the quantification of plant hormones and transcriptome and metabolome assays. The results show that the different modes of treatments significantly influence the contents of JA and SA in time following treatment. Three types of differentially accumulated metabolites (DAMs) were found to be involved in the initial response, namely phenolic acids, lipids, and flavonoids. Weighted gene co-expression network analysis indicated that 722 differentially expressed genes (DEGs) are positively related to feeding stimulation and the specific enriched pathways are plant hormone signal transduction and flavonoid biosynthesis, among others. Two TIFY transcription factors (PtTIFY54 and PtTIFY22) and a MYB transcription factor (PtMYB26) were found to be involved in the interaction between plant hormones, mainly in the context of JA signal transduction and flavonoid biosynthesis. The results of this study provide an insight into how JA activates, serving as a reference for understanding the molecular mechanisms of resistance formation in conifers responding to mandibulate insects.

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Phytotoxic effect and molecular mechanism induced by graphene towards alfalfa (Medicago sativa L.) by integrating transcriptomic and metabolomics analysis

Cited by 15 publications

References 66 publications

Effect of graphene on soybean root colonization by Bradyrhizobium strains

Effect of graphene on soybean root colonization by Bradyrhizobium strains

Exogenously Applied Rohitukine Inhibits Photosynthetic Processes, Growth and Induces Antioxidant Defense System in Arabidopsis thaliana

The Key Role of Plant Hormone Signaling Transduction and Flavonoid Biosynthesis Pathways in the Response of Chinese Pine (Pinus tabuliformis) to Feeding Stimulation by Pine Caterpillar (Dendrolimus tabulaeformis)

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