2015
DOI: 10.1016/j.expneurol.2015.02.021
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PI3Kγ deficiency enhances seizures severity and associated outcomes in a mouse model of convulsions induced by intrahippocampal injection of pilocarpine

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Cited by 11 publications
(4 citation statements)
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References 68 publications
(69 reference statements)
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“…A red X indicates inhibition. (VonDran et al, 2014;Lima et al, 2015;Sanna et al, 2017). Thus, we evaluated the protein levels of BDNF by using a Western blot analysis to test whether an increase in neuroprotection after cerebrolysin administration is correlated with increases in BDNF.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…A red X indicates inhibition. (VonDran et al, 2014;Lima et al, 2015;Sanna et al, 2017). Thus, we evaluated the protein levels of BDNF by using a Western blot analysis to test whether an increase in neuroprotection after cerebrolysin administration is correlated with increases in BDNF.…”
Section: Discussionmentioning
confidence: 99%
“…Several studies have demonstrated that neurotrophic factors, including NGF and BDNF, are elevated after status epilepticus, which promotes the survival of neurons post-seizure ( VonDran et al, 2014 ; Lima et al, 2015 ; Sanna et al, 2017 ). Thus, we evaluated the protein levels of BDNF by using a Western blot analysis to test whether an increase in neuroprotection after cerebrolysin administration is correlated with increases in BDNF.…”
Section: Discussionmentioning
confidence: 99%
“…Fluoro-Jade C (FJC) is an anionic fluorescein derivate used to label degenerating neurons 38 . Although the exact mechanism is not known, different studies have demonstrated that FJC is a reliable dye used to stain dying neurons 75 76 77 78 79 . The hippocampal slices were washed 3 times in PBS for 30 minutes and mounted on gelatinized slides.…”
Section: Methodsmentioning
confidence: 99%
“…After, blocking solution [BSA (4%), Triton X (0.5%) in TBS] was added to the slices for 2 h, and they were incubated overnight with anti-Iba-1 primary antibody (1:500; Wako Chemicals, Osaka, Japan). On the next day, the slices were incubated with the secondary antibody Alexa Fluor 594 anti-rabbit (1:1000; Invitrogen, Carlsbad, CA, USA) for 1 h, washed, mounted in gelatinized slides and coverslipped with Fluoromount media (Sigma-Aldrich, St. Louis, MO, USA) 77 .…”
Section: Methodsmentioning
confidence: 99%