Pickering emulsion stabilized by Chinese Yam polysaccharides PLGA for enhanced humoral and cellular immune responses

Zhang, Yue; Fan, Zexiao; Gu, Peng; Mao, Ningning; Song, Peng; Song, Zuchen; Liu, Zhenguang; Yang, Yang; Wang, Deyun

doi:10.1016/j.colsurfb.2022.112746

Cited by 5 publications

(7 citation statements)

References 45 publications

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“…The immune cells further trigger robust humoral and cellular immune responses. ,− PLGA-stabilized Pickering emulsions can encapsulate the antigen and the adjuvant, providing an attractive and effective platform for drug and vaccine delivery . Our previous study found that the Chinese yam polysaccharide PLGA-stabilized Pickering emulsion (CYP-PPAS) has controlled release properties while promoting dendritic cell uptake and serving as a safe adjuvant to enhance the immune response. , Here, we compared the adjuvant activity of the cationic nanoparticle-stabilized PEI-CYP-PPAS Pickering emulsion with the CYP-PPAS Pickering emulsion and the Alum adjuvant on chickens immunized with the H9N2 influenza vaccine.…”

Section: Discussionmentioning

confidence: 99%

“…20 Our previous study found that the Chinese yam polysaccharide PLGA-stabilized Pickering emulsion (CYP-PPAS) has controlled release properties while promoting dendritic cell uptake and serving as a safe adjuvant to enhance the immune response. 31,33 Here, we compared the adjuvant activity of the cationic nanoparticlestabilized PEI-CYP-PPAS Pickering emulsion with the CYP-PPAS Pickering emulsion and the Alum adjuvant on chickens immunized with the H9N2 influenza vaccine.…”

Section: ■ Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Our previous research revealed that Chinese yam polysaccharide PLGA-stabilized Pickering emulsions (CYP-PPAS) provide controlled drug and antigen release properties while promoting cellular uptake and enhancing the immune response. 31 Herein, we designed a cationic nanoparticlestabilized vaccine delivery system of polyethyleneiminemodified CYP-PLGA nanoparticle-stabilized Pickering emulsion (PEI-CYP-PPAS). Meanwhile, we applied this cationic Pickering emulsion of PEI-CYP-PPAS, CYP-PPAS, and aluminum adjuvant as adjuvants for the development of H9N2 Avian influenza vaccines to compare their adjuvant activities on immune response after vaccination with chickens.…”

Section: ■ Introductionmentioning

confidence: 99%

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Cationic Nanoparticle-Stabilized Vaccine Delivery System for the H9N2 Vaccine to Promote Immune Response in Chickens

Zhang

Zhu

et al. 2023

Mol. Pharmaceutics

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Chinese yam polysaccharides (CYPs) have received wide attention for their immunomodulatory activity. Our previous studies had discovered that the Chinese yam polysaccharide PLGA-stabilized Pickering emulsion (CYP-PPAS) can serve as an efficient adjuvant to trigger powerful humoral and cellular immunity. Recently, positively charged nano-adjuvants are easily taken up by antigen-presenting cells, potentially resulting in lysosomal escape, the promotion of antigen cross-presentation, and the induction of CD8 T-cell response. However, reports on the practical application of cationic Pickering emulsions as adjuvants are very limited. Considering the economic damage and public-health risks caused by the H9N2 influenza virus, it is urgent to develop an effective adjuvant for boosting humoral and cellular immunity against influenza virus infection. Here, we applied polyethyleneimine-modified Chinese yam polysaccharide PLGA nanoparticles as particle stabilizers and squalene as the oil core to fabricate a positively charged nanoparticle-stabilized Pickering emulsion adjuvant system (PEI-CYP-PPAS). The cationic Pickering emulsion of PEI-CYP-PPAS was utilized as an adjuvant for the H9N2 Avian influenza vaccine, and the adjuvant activity was compared with the Pickering emulsion of CYP-PPAS and the commercial adjuvant (aluminum adjuvant). The PEI-CYP-PPAS, with a size of about 1164.66 nm and a ζ potential of 33.23 mV, could increase the H9N2 antigen loading efficiency by 83.99%. After vaccination with Pickering emulsions based on H9N2 vaccines, PEI-CYP-PPAS generated higher HI titers and stronger IgG antibodies than CYP-PPAS and Alum and increased the immune organ index of the spleen and bursa of Fabricius without immune organ injury. Moreover, treatment with PEI-CYP-PPAS/H9N2 induced CD4+ and CD8+ T-cell activation, a high lymphocyte proliferation index, and increased cytokine expression of IL-4, IL-6, and IFN-γ. Thus, compared with the CYP-PPAS and aluminum adjuvant, the cationic nanoparticle-stabilized vaccine delivery system of PEI-CYP-PPAS was an effective adjuvant for H9N2 vaccination to elicit powerful humoral and cellular immune responses.

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Section: Discussionmentioning

confidence: 99%

Section: ■ Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Cationic Nanoparticle-Stabilized Vaccine Delivery System for the H9N2 Vaccine to Promote Immune Response in Chickens

Zhang

Zhu

et al. 2023

Mol. Pharmaceutics

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“…[ 47 ] Yam polysaccharide, the active ingredient of yam, induces humoral immunity and Th1/Th2 immune responses, enhanced activation of CD4+ and CD8+ T lymphocyte subsets, and promoted activation of cytotoxic T lymphocyte responses. [ 48 ] After SARS-CoV infection, dysregulation of responses to numerous proinflammatory cytokines (e.g., IL-6, IL-8, IL-1β, and tumor necrosis factor-α [TNF-α]) and chemokines (e.g., CCL-2, CCL-3, CCL-5, IP-10) due to leading to a storm of inflammatory cytokines that exacerbate dyspnea and increase mortality. [ 49 , 50 ] The development of acute respiratory distress syndrome is inextricably linked to increased endothelial and epithelial permeability of the lung, in addition to excessive inflammation.…”

Section: Discussionmentioning

confidence: 99%

Efficacy and safety of Qi and Blood Tonic Chinese Medicines in the treatment of COVID-19: A protocol for systematic review and meta-analysis

Yang

Zhang

et al. 2022

Medicine

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Background: Coronavirus disease in 2019 (COVID-19) is a sudden public event affecting all human beings, with the rapid transmission, extensive groups affected, many complications, and high mortality. Traditional Chinese Medicine has a long history of preventing and treating infectious diseases, and numerous studies have shown that Traditional Chinese Medicine, especially herbal medicine, has a positive effect on the prevention, treatment, and post-healing recovery of this COVID-19, and herbal medicines to supplement qi and blood often occupy a certain proportion of it. However, there is no relevant meta-analysis to date. Therefore, this study aims to evaluate the efficacy and safety of qi and blood tonic herbal medicines in the treatment of COVID-19 through Systematic Review and meta-analysis to provide a reference basis for widespread clinical application. Methods: We will search from the following databases for the period from the time of database construction to March 1st, 2023. The English databases include: PubMed, MEDLINE, EMBASE, Cochrane library, WOS, Google Scholar, and CENTRAL; The Chinese databases include: China National Knowledge Infrastructure, China Biomedical Literature Database, Technology Journal Database, and Wanfang. Randomized controlled trials in English or Chinese that include Chinese herbal medicines for tonifying Qi and Blood in the treatment of patients with COVID-19 will be included. Data were independently screened and collected by 2 investigators. The risk of bias for each trial was assessed using the Cochrane Risk of Bias Tool 2.0. RevMan 5.3 software was used for the meta-analysis of the data. Primary outcome indicators included cure, mortality, and exacerbation rates (change in disease severity category, patient admission to ICU, etc.). Secondary outcome indicators included recovery rate or duration of major symptoms (e.g., fever, cough, fatigue, and weakness, etc.), rate or duration of nucleic acid conversion for severe acute respiratory syndrome coronavirus-2, improvement or recovery of chest CT performance, length of hospital stay, and other adverse events. Results: This protocol adheres to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses-P guidelines to ensure clarity and completeness of reporting in all phases of the systematic review. Conclusion: This study will provide evidence regarding the efficacy and safety of Qi and Blood Tonic Chinese Medicines for the treatment of COVID-19. PROSPERO registration number: CRD42022361822 (https://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42022361822).

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Prospects of yam (Dioscorea) polysaccharides: Structural features, bioactivities and applications

Zhang,

Wang,

Zhang

et al. 2024

Food Chemistry

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Pickering emulsion stabilized by Chinese Yam polysaccharides PLGA for enhanced humoral and cellular immune responses

Cited by 5 publications

References 45 publications

Cationic Nanoparticle-Stabilized Vaccine Delivery System for the H9N2 Vaccine to Promote Immune Response in Chickens

Cationic Nanoparticle-Stabilized Vaccine Delivery System for the H9N2 Vaccine to Promote Immune Response in Chickens

Efficacy and safety of Qi and Blood Tonic Chinese Medicines in the treatment of COVID-19: A protocol for systematic review and meta-analysis

Prospects of yam (Dioscorea) polysaccharides: Structural features, bioactivities and applications

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