Picophytoplankton identification by flow cytometry and high-throughput sequencing in a clean reservoir

Ning, Man; Li, Huimin; Xu, Zheng; Chen, Lei; He, Yiliang

doi:10.1016/j.ecoenv.2021.112216

Cited by 13 publications

(3 citation statements)

References 58 publications

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“…fluorescence, size) which were also used to identify different groups of phytoplankton (see e.g. (Ning et al, 2021; Read et al, 2014; Thyssen et al, 2022) and supplementary material fig. S1).…”

Section: Methodsmentioning

confidence: 99%

Picophytoplankton dominate phytoplankton communities in the Elbe estuary in terms of cell counts

Martens,

Biederbick,

Schaum

2024

Preprint

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Summary(1)Picophytoplankton are important primary producers, but not always adequately recognized, e.g. due to methodological limitations.(2)In this study, we combined flow cytometry and metabarcoding to investigate seasonal and spatial patterns of picophytoplankton abundance and community composition in the Elbe estuary.(3)Picophytoplankton (mostly picoeukaryotes such asMychonastesandMinidiscus)contributed on average 70 % (SD = 14 %) to the total phytoplankton counts. In the summer picocyanobacteria (e.g.Synechococcus) played a more significant role. The contributions of picophytoplankton to the total phytoplankton were particularly high from summer to winter as well as in the mid estuary. However, at salinities of around 10 PSU in the mixing area the proportion of picophytoplankton was comparably low (average 49 %, SD = 13 %).(4)Our results indicate that picophytoplankton prevail in the Elbe estuary year-round with respect to cell counts. Picophytoplankton could occupy important niche positions to maintain primary production under extreme conditions where larger phytoplankton might struggle (e.g. at high or low temperature and high turbidity), and also benefit from high nutrient availability here. However, we did not find evidence that they played a particularly significant role at the salinity interface. Our study highlights the importance of including picophytoplankton when assessing estuarine phytoplankton as has been suggested for other ecosystems such as oceans.

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Section: Methodsmentioning

confidence: 99%

Picophytoplankton dominate phytoplankton communities in the Elbe estuary in terms of cell counts

Martens,

Biederbick,

Schaum

2024

Preprint

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“…Compared with PCY, PPEs have much more complex community structures and functions, and they are abundant in eutrophic lake ecosystems, such as Lake Chaohu and Lake Taihu ( 4 ). Flow cytometric (FCM) sorting and high-throughput sequencing have been used to investigate picophytoplankton community structures in recent years ( 5 , 6 ). Early relevant studies were focused on marine ecosystems ( 2 , 3 , 7 ), but most water samples could not be immediately analyzed unless the sampling ship was equipped with FCM ( 8 ).…”

Section: Introductionmentioning

confidence: 99%

Optimization of Preservation Methods Provides Insights into Photosynthetic Picoeukaryotes in Lakes

et al. 2022

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“…Nano-and picophytoplankton (under 10µm) are often most commonly observed by flow-cytometry which can detect cells on gross characteristics, commonly size and pigment, although they represent thousands of species. Studies combining flow-cytometry detection of picoand nanophytoplankton with high throughput sequencing revealed distinct phenology and habitats [21]. Aureococcus anophagefferens is pelagophyte, single-celled picophytoplankton of 2µm in size recorded to grow to bloom level in the east coast of USA and South Africa, in what is known as a brown tide event due to toxins that kill shellfish and the poor light conditions resulting from their growth [22].…”

Section: Introduction Outlinementioning

confidence: 99%

Composition and Patterns of Taxa Assemblages in the Western Channel Assessed by 18S Sequencing, Microscopy and Flow Cytometry

Stern¹,

Picard²,

Clarke³

et al. 2022

Preprint

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Plankton monitoring by microscopy offers long-term ecological perspective of plankton com-munities but different detection approaches are biased uniquely. Genetic identification of marine plankton has become standard but is still not used in routine monitoring. This study assessed the diversity of plankton taxa using 18S high throughput sequencing from 2011-2012 from small-volume (~200ml) samples from the Water and Microplankton Sampler (WaMS) deployed on the Continuous Plankton Recorder platform (CPR). The 18S-HTS survey revealed a bias towards heterotrophic taxa, and phototrophs under 10µm within the photosynthetic community. In comparison with phytoplankton microscopic counts from the CPR survey and Western Channel Observatory station L4, only 8-12 taxonomic families were common to all three surveys, with a bias towards larger diatoms and dinoflagellate taxa in microscopy surveys. The WaMS survey detected a contrasting but complementary taxa set to that of microscopic surveys. Additional Quantitative PCR was carried out on the picoeukaryotic pelagophyte, Aureococcus anophagefferens, and the nanoeukaryotic potential harmful algae, Pseudo-nitzschia delicatissima, from 2011-2013. This confirmed the persistence presence of A. anophagefferens in the Western Channel and an elevated abundance of both species in 2011. Species specific seasonality were distinct from those of aggregrate phytoplankton groups.

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Picophytoplankton identification by flow cytometry and high-throughput sequencing in a clean reservoir

Cited by 13 publications

References 58 publications

Picophytoplankton dominate phytoplankton communities in the Elbe estuary in terms of cell counts

Picophytoplankton dominate phytoplankton communities in the Elbe estuary in terms of cell counts

Optimization of Preservation Methods Provides Insights into Photosynthetic Picoeukaryotes in Lakes

Composition and Patterns of Taxa Assemblages in the Western Channel Assessed by 18S Sequencing, Microscopy and Flow Cytometry

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