Piezo1 forms mechanosensitive ion channels in the human MCF-7 breast cancer cell line

Li, Chouyang; Rezania, Simin; Kammerer, Sarah; Sokołowski, A.; DeVaney, Trevor; Gorischek, Astrid; Jahn, Stephan; Hackl, Hubert; Gröschner, Klaus; Windpassinger, Christian; Malle, Ernst; Bauernhofer, Thomas; Schultze-Seemann, Wolfgang

doi:10.1038/srep08364

Cited by 141 publications

(132 citation statements)

References 49 publications

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“…The mRNA level of PIEZO1 was shown relatively low in a benign breast epithelial cell line, but highly expressed in the malign MCF‐7 cells and primary tumors. Increase of PIEZO1 correlated with poor overall survival in patients with breast cancer . Besides, PIEZO1 was among the highest expressed genes in thyroid cancers following Iodine‐131 exposure .…”

Section: Discussionmentioning

confidence: 93%

“…Additionally, recent studies addressed that when it comes to epithelial cell crowding and cytoskeletal contractions, PIEZO1 could be activated to maintain cell homeostasis of epithelial monolayers . Concerning the distinct role of PIEZO1 in epithelial cells, the functional role PIEZO1 have been investigated in epithelia derived cancers . By performing knockdown experiments, lung cancer cells initiated an undirected migration mode and acquired a more aggressive phenotype to increase cell motility .…”

Section: Introductionmentioning

confidence: 99%

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PIEZO1 functions as a potential oncogene by promoting cell proliferation and migration in gastric carcinogenesis

Zhang

Zhou

Huang

et al. 2018

Molecular Carcinogenesis

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Gastric cancer (GC) is one of the most common malignancies in Asian areas. PIEZO1 has been implied to regulate epithelial homeostasis to play an important role in physiological processes. It is also related to tumor initiation and progression. However, the role of PIEZO1 has not yet been explored in GC. The expression of PIEZO1 in GC cell lines and primary samples was determined by qRT-PCR and Western blot. The clinical correlation of PIEZO1 in GC was evaluated by immunohistochemistry on tissue microarray. The oncogenic role of PIEZO1 was demonstrated in gastric tumorigenesis through a series of functional assays, including cell proliferation, cell invasion, and flow cytometry analysis. Drug sensitivity was also assessed by PIEZO1 knockdown experiment. PIEZO1 exhibited an upregulation in most of the GC cell lines and primary samples compared with non-tumorous gastric epithelial tissues. Increase of PIEZO1 was associated with poor disease specific survival. PIEZO1 knockdown led to inhibitory effect by suppressing cell proliferation and invasion and inhibiting xenograft formation. Decreased PIEZO1 enhanced the sensitivity of Cisplatin or 5-FU treatment. Morphological alteration was also observed in siPIEZO1 treated cells. GTP-Rac1 showed accumulated activated form, while total-RhoA was decreased accompanied with PIEZO1 knockdown. In the present study, PIEZO1 is required for cell proliferation, migration and invasion to promote GC progression. PIEZO1 also maintains cellular morphology related to GC cell motility by regulating the activity of Rho GTPase family members. Our data not only suggested a novel prognostic marker, but also provided a useful clinical therapeutic target for GC.

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Section: Discussionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

PIEZO1 functions as a potential oncogene by promoting cell proliferation and migration in gastric carcinogenesis

Zhang

Zhou

Huang

et al. 2018

Molecular Carcinogenesis

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“…To test whether the Piezo1 channel plays a role in regulating hDP‐MSC migration, as recently reported in endothelial cell and various cancer cells, we used the wound healing assay to examine the effect of Yoda1 on cell migration (Figure ). Inclusion of 0.1‐1 μM Yoda1 in the culture medium resulted in a concentration‐dependent increase in cell migration, with 0.3 and 1 μM Yoda1 significantly accelerating cell migration at 24, 48, and 72 hours, and such stimulation was observed in hDP‐MSCs from all four donors, with some variations among the different donors (Figure A‐F).…”

Section: Resultsmentioning

confidence: 99%

“…Furthermore, recent studies have reported that mechanical or chemical activation of the Piezo1 channel induces ATP release from endothelial cells, urothelial cells, and red blood cells . There is also compelling evidence to support a critical role for the Piezo1 channel in regulating cell migration in endothelial cells, breast cancer cells, gastric cancer cells, and small lung cancer cells . Recent studies have also shown the expression of the Piezo1 channel in human bone marrow‐derived MSCs (BM‐MSCs) and rat dental pulp‐derived stem cells (DP‐MSCs) .…”

Section: Introductionmentioning

confidence: 99%

Chemical activation of the Piezo1 channel drives mesenchymal stem cell migration via inducing ATP release and activation of P2 receptor purinergic signaling

Mousawi

Peng

et al. 2020

Stem Cells

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In this study, we examined the Ca2+‐permeable Piezo1 channel, a newly identified mechanosensing ion channel, in human dental pulp‐derived mesenchymal stem cells (MSCs) and hypothesized that activation of the Piezo1 channel regulates MSC migration via inducing ATP release and activation of the P2 receptor purinergic signaling. The Piezo1 mRNA and protein were readily detected in hDP‐MSCs from multiple donors and, consistently, brief exposure to Yoda1, the Piezo1 channel‐specific activator, elevated intracellular Ca2+ concentration. Yoda1‐induced Ca2+ response was inhibited by ruthenium red or GsMTx4, two Piezo1 channel inhibitors, and also by Piezo1‐specific siRNA. Brief exposure to Yoda1 also induced ATP release. Persistent exposure to Yoda1 stimulated MSC migration, which was suppressed by Piezo1‐specific siRNA, and also prevented by apyrase, an ATP scavenger, or PPADS, a P2 generic antagonist. Furthermore, stimulation of MSC migration induced by Yoda1 as well as ATP was suppressed by PF431396, a PYK2 kinase inhibitor, or U0126, an inhibitor of the mitogen‐activated protein kinase MEK/ERK signaling pathway. Collectively, these results suggest that activation of the Piezo1 channel stimulates MSC migration via inducing ATP release and subsequent activation of the P2 receptor purinergic signaling and downstream PYK2 and MEK/ERK signaling pathways, thus revealing novel insights into the molecular and signaling mechanisms regulating MSC migration. Such findings provide useful information for evolving a full understanding of MSC migration and homing and developing strategies to improve MSC‐based translational applications.

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“…Highly metastatic breast cancer cells exhibit high Piezo1 expression, which has been correlated with worse outcomes in one patient population [63]. Metastatic breast cancer cells, invasive melanoma cells and gastric cancer cells exhibited reduced migration when Piezol was knocked down or pharmacologically inhibited [29,63,64]. In these cell types, higher Piezo1 activity has been proposed to underlie greater migration associated with the metastatic state.…”

Section: Effect Of Piezo1 Activity On the Cytoskeletonmentioning

confidence: 99%

How cells channel their stress: Interplay between Piezo1 and the cytoskeleton

Nourse

Pathak

2017

Seminars in Cell & Developmental Biology

191

160

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Cells constantly encounter mechanical stimuli in their environment, such as dynamic forces and mechanical features of the extracellular matrix. These mechanical cues are transduced into biochemical signals, and integrated with genetic and chemical signals to modulate diverse physiological processes. Cells also actively generate forces to internally transport cargo, to explore the physical properties of their environment and to spatially position themselves and other cells during development. Mechanical forces are therefore central to development, homeostasis, and repair. Several molecular and biophysical strategies are utilized by cells for detecting and generating mechanical forces. Here we discuss an important class of molecules involved in sensing and transducing mechanical forces – mechanically-activated ion channels. We focus primarily on the Piezo1 ion channel, and examine its relationship with the cellular cytoskeleton.

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Piezo1 forms mechanosensitive ion channels in the human MCF-7 breast cancer cell line

Cited by 141 publications

References 49 publications

PIEZO1 functions as a potential oncogene by promoting cell proliferation and migration in gastric carcinogenesis

PIEZO1 functions as a potential oncogene by promoting cell proliferation and migration in gastric carcinogenesis

Chemical activation of the Piezo1 channel drives mesenchymal stem cell migration via inducing ATP release and activation of P2 receptor purinergic signaling

How cells channel their stress: Interplay between Piezo1 and the cytoskeleton

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